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Protein

Quinolinate synthase A

Gene

nadA

Organism
Mycobacterium africanum (strain GM041182)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationSAAS annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotationSAAS annotation
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per subunit.

Pathway: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521IminoaspartateUniRule annotation
Binding sitei69 – 691Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei140 – 1401IminoaspartateUniRule annotation
Binding sitei157 – 1571IminoaspartateUniRule annotation
Binding sitei227 – 2271IminoaspartateUniRule annotation
Binding sitei255 – 2551IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMAFR572418:GJCK-1622-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotationImported
Ordered Locus Names:MAF_16060Imported
OrganismiMycobacterium africanum (strain GM041182)Imported
Taxonomic identifieri572418 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000000298 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF8M859.
SMRiF8M859. Positions 32-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

KOiK03517.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

F8M859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLNRTDTL VDELTADITN TPLGYGGVDG DERWAAEIRR LAHLRGATVL
60 70 80 90 100
AHNYQLPAIQ DVADHVGDSL ALSRVAAEAP EDTIVFCGVH FMAETAKILS
110 120 130 140 150
PHKTVLIPDQ RAGCSLADSI TPDELRAWKD EHPGAVVVSY VNTTAAVKAL
160 170 180 190 200
TDICCTSSNA VDVVASIDPD REVLFCPDQF LGAHVRRVTG RKNLHVWAGE
210 220 230 240 250
CHVHAGINGD ELADQARAHP DAELFVHPEC GCATSALYLA GEGAFPAERV
260 270 280 290 300
KILSTGGMLE AAHTTRARQV LVATEVGMLH QLRRAAPEVD FRAVNDRASC
310 320 330 340
KYMKMITPAA LLRCLVEGAD EVHVDPGIAA SGRRSVQRMI EIGHPGGGE
Length:349
Mass (Da):37,408
Last modified:September 21, 2011 - v1
Checksum:i3053D1945CBE5288
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR878060 Genomic DNA. Translation: CCC26678.1.
RefSeqiWP_003407931.1. NC_015758.1.
YP_004723292.1. NC_015758.1.

Genome annotation databases

EnsemblBacteriaiCCC26678; CCC26678; MAF_16060.
KEGGimaf:MAF_16060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR878060 Genomic DNA. Translation: CCC26678.1.
RefSeqiWP_003407931.1. NC_015758.1.
YP_004723292.1. NC_015758.1.

3D structure databases

ProteinModelPortaliF8M859.
SMRiF8M859. Positions 32-342.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC26678; CCC26678; MAF_16060.
KEGGimaf:MAF_16060.

Phylogenomic databases

KOiK03517.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciMAFR572418:GJCK-1622-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Genome of Mycobacterium Africanum West African 2 Reveals a Lineage-Specific Locus and Genome Erosion Common to the M. tuberculosis Complex."
    Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
    PLoS Negl. Trop. Dis. 6:e1552-e1552(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: GM041182Imported.

Entry informationi

Entry nameiF8M859_MYCA0
AccessioniPrimary (citable) accession number: F8M859
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: June 24, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.