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F8M6H6

- F8M6H6_MYCA0

UniProt

F8M6H6 - F8M6H6_MYCA0

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Protein

Coenzyme F420:L-glutamate ligase

Gene
fbiB, MAF_32730
Organism
Mycobacterium africanum (strain GM041182)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives By similarity.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese By similarity.UniRule annotation
Monovalent cation. The ion could be potassium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501GTP By similarityUniRule annotation
Binding sitei55 – 551GTP By similarityUniRule annotation
Metal bindingi109 – 1091Divalent metal cation 1 By similarityUniRule annotation
Binding sitei112 – 1121GTP By similarityUniRule annotation
Metal bindingi150 – 1501Divalent metal cation 1 By similarityUniRule annotation
Metal bindingi151 – 1511Divalent metal cation 2 By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 234GTP By similarityUniRule annotation

GO - Molecular functioni

  1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
  2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
  3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
  2. F420-0 metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMAFR572418:GJCK-3297-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligase
Coenzyme F420-1:gamma-L-glutamate ligase
Gene namesi
Name:fbiBUniRule annotationImported
Ordered Locus Names:MAF_32730Imported
OrganismiMycobacterium africanum (strain GM041182)Imported
Taxonomic identifieri572418 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000000298: Chromosome

Structurei

3D structure databases

ProteinModelPortaliF8M6H6.
SMRiF8M6H6. Positions 12-227, 260-444.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 244244F420-0:gamma-glutamyl ligase By similarityUniRule annotationAdd
BLAST
Regioni245 – 448204Unknown By similarityUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CofE family.UniRule annotation

Phylogenomic databases

KOiK12234.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.

Sequencei

Sequence statusi: Complete.

F8M6H6-1 [UniParc]FASTAAdd to Basket

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MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS    50
KVVSKCEGRL VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG 100
LVQAAAGVDG SNVGRSELAL LPVDPDASAA TLRAGLRERL GVTVAVVITD 150
TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR DPYGNELVVT EVAVADEIAA 200
AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL FWLGTAEALE 250
LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL 300
QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP 350
MLVPDGAHSY PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS 400
TIFAADLVRD ELDLPVDWEP LGAIAIGYAD EPSGLRDPVP AADLLILK 448
Length:448
Mass (Da):47,578
Last modified:September 21, 2011 - v1
Checksum:i8C6DB75DE6E86851
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeqiYP_004724914.1. NC_015758.1.

Genome annotation databases

EnsemblBacteriaiCCC28343; CCC28343; MAF_32730.
GeneIDi10957411.
KEGGimaf:MAF_32730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1 .
RefSeqi YP_004724914.1. NC_015758.1.

3D structure databases

ProteinModelPortali F8M6H6.
SMRi F8M6H6. Positions 12-227, 260-444.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCC28343 ; CCC28343 ; MAF_32730 .
GeneIDi 10957411.
KEGGi maf:MAF_32730.

Phylogenomic databases

KOi K12234.

Enzyme and pathway databases

UniPathwayi UPA00071 .
BioCyci MAFR572418:GJCK-3297-MONOMER.

Family and domain databases

Gene3Di 3.40.109.10. 1 hit.
HAMAPi MF_01259. F420_ligase_FbiB.
InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view ]
Pfami PF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view ]
SUPFAMi SSF55469. SSF55469. 1 hit.
TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Mycobacterium africanum West African 2 reveals a lineage-specific locus and genome erosion common to the M. tuberculosis complex."
    Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
    PLoS Negl. Trop. Dis. 6:E1552-E1552(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: GM041182Imported.

Entry informationi

Entry nameiF8M6H6_MYCA0
AccessioniPrimary (citable) accession number: F8M6H6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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