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F8M6H6

- F8M6H6_MYCA0

UniProt

F8M6H6 - F8M6H6_MYCA0

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Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium africanum (strain GM041182)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation
Monovalent cation. The ion could be potassium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501GTPUniRule annotation
Binding sitei55 – 551GTPUniRule annotation
Metal bindingi109 – 1091Divalent metal cation 1UniRule annotation
Binding sitei112 – 1121GTPUniRule annotation
Metal bindingi150 – 1501Divalent metal cation 1UniRule annotation
Metal bindingi151 – 1511Divalent metal cation 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 234GTPUniRule annotation

GO - Molecular functioni

  1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
  2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
  3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
  2. F420-0 metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMAFR572418:GJCK-3297-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligaseUniRule annotation
Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
Gene namesi
Name:fbiBUniRule annotationImported
Ordered Locus Names:MAF_32730Imported
OrganismiMycobacterium africanum (strain GM041182)Imported
Taxonomic identifieri572418 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000000298: Chromosome

Structurei

3D structure databases

ProteinModelPortaliF8M6H6.
SMRiF8M6H6. Positions 12-227, 260-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 244244F420-0:gamma-glutamyl ligaseUniRule annotationAdd
BLAST
Regioni245 – 448204UnknownUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CofE family.UniRule annotation

Phylogenomic databases

KOiK12234.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.

Sequencei

Sequence statusi: Complete.

F8M6H6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS
60 70 80 90 100
KVVSKCEGRL VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG
110 120 130 140 150
LVQAAAGVDG SNVGRSELAL LPVDPDASAA TLRAGLRERL GVTVAVVITD
160 170 180 190 200
TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR DPYGNELVVT EVAVADEIAA
210 220 230 240 250
AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL FWLGTAEALE
260 270 280 290 300
LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL
310 320 330 340 350
QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP
360 370 380 390 400
MLVPDGAHSY PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS
410 420 430 440
TIFAADLVRD ELDLPVDWEP LGAIAIGYAD EPSGLRDPVP AADLLILK
Length:448
Mass (Da):47,578
Last modified:September 21, 2011 - v1
Checksum:i8C6DB75DE6E86851
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeqiYP_004724914.1. NC_015758.1.

Genome annotation databases

EnsemblBacteriaiCCC28343; CCC28343; MAF_32730.
GeneIDi10957411.
KEGGimaf:MAF_32730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1 .
RefSeqi YP_004724914.1. NC_015758.1.

3D structure databases

ProteinModelPortali F8M6H6.
SMRi F8M6H6. Positions 12-227, 260-444.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCC28343 ; CCC28343 ; MAF_32730 .
GeneIDi 10957411.
KEGGi maf:MAF_32730.

Phylogenomic databases

KOi K12234.

Enzyme and pathway databases

UniPathwayi UPA00071 .
BioCyci MAFR572418:GJCK-3297-MONOMER.

Family and domain databases

Gene3Di 3.40.109.10. 1 hit.
HAMAPi MF_01259. F420_ligase_FbiB.
InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view ]
Pfami PF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view ]
SUPFAMi SSF55469. SSF55469. 1 hit.
TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of Mycobacterium africanum West African 2 reveals a lineage-specific locus and genome erosion common to the M. tuberculosis complex."
    Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
    PLoS Negl. Trop. Dis. 6:E1552-E1552(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: GM041182Imported.

Entry informationi

Entry nameiF8M6H6_MYCA0
AccessioniPrimary (citable) accession number: F8M6H6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3