Coenzyme F420:L-glutamate ligase - Mycobacterium africanum (strain GM041182)

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F8M6H6 (F8M6H6_MYCA0) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 15. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Coenzyme F420:L-glutamate ligase HAMAP-Rule MF_01259
EC=6.3.2.31 HAMAP-Rule MF_01259
EC=6.3.2.34 HAMAP-Rule MF_01259

Alternative name(s):
Coenzyme F420-0:L-glutamate ligase HAMAP-Rule MF_01259
Coenzyme F420-1:gamma-L-glutamate ligase HAMAP-Rule MF_01259

Gene names

Name:	fbiB HAMAP-Rule MF_01259 EMBL CCC28343.1
Synonyms:	cofE
Ordered Locus Names:	MAF_32730

Organism

Mycobacterium africanum (strain GM041182) [Complete proteome] [HAMAP] EMBL CCC28343.1

Taxonomic identifier

572418 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives By similarity. HAMAP-Rule MF_01259
Catalytic activity	GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1. HAMAP-Rule MF_01259 GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n. HAMAP-Rule MF_01259 GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2. HAMAP-Rule MF_01259
Cofactor	Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese By similarity. HAMAP-Rule MF_01259 Monovalent cation. The ion could be potassium By similarity. HAMAP-Rule MF_01259
Pathway	Cofactor biosynthesis; coenzyme F420 biosynthesis. HAMAP-Rule MF_01259
Sequence similarities	In the N-terminal section; belongs to the CofE family. HAMAP-Rule MF_01259

Ontologies

Keywords
Ligand	GTP-binding HAMAP-Rule MF_01259 Magnesium HAMAP-Rule MF_01259 Manganese HAMAP-Rule MF_01259 Metal-binding HAMAP-Rule MF_01259 Nucleotide-binding Potassium HAMAP-Rule MF_01259
Molecular function	Ligase HAMAP-Rule MF_01259
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	F420-0 metabolic process Inferred from electronic annotation. Source: HAMAP coenzyme biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation-reduction process Inferred from electronic annotation. Source: GOC
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW coenzyme F420-0 gamma-glutamyl ligase activity Inferred from electronic annotation. Source: InterPro coenzyme F420-0:L-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP coenzyme F420-1:gamma-L-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

20 – 23

GTP By similarity HAMAP-Rule MF_01259

Region

1 – 244

244

F420-0:gamma-glutamyl ligase By similarity HAMAP-Rule MF_01259

Region

245 – 448

204

Unknown By similarity HAMAP-Rule MF_01259

Sites

Metal binding

109

Divalent metal cation 1 By similarity HAMAP-Rule MF_01259

Metal binding

150

Divalent metal cation 1 By similarity HAMAP-Rule MF_01259

Metal binding

151

Divalent metal cation 2 By similarity HAMAP-Rule MF_01259

Binding site

GTP By similarity HAMAP-Rule MF_01259

Binding site

GTP By similarity HAMAP-Rule MF_01259

Binding site

112

GTP By similarity HAMAP-Rule MF_01259

Sequences

Sequence

Length

Mass (Da)

Tools

F8M6H6 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 8C6DB75DE6E86851
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FASTA

448

47,578

        10         20         30         40         50         60 
MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS KVVSKCEGRL 

        70         80         90        100        110        120 
VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG LVQAAAGVDG SNVGRSELAL 

       130        140        150        160        170        180 
LPVDPDASAA TLRAGLRERL GVTVAVVITD TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR 

       190        200        210        220        230        240 
DPYGNELVVT EVAVADEIAA AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL 

       250        260        270        280        290        300 
FWLGTAEALE LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL 

       310        320        330        340        350        360 
QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP MLVPDGAHSY 

       370        380        390        400        410        420 
PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS TIFAADLVRD ELDLPVDWEP 

       430        440 
LGAIAIGYAD EPSGLRDPVP AADLLILK

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References

[1]

"The genome of Mycobacterium africanum West African 2 reveals a lineage-specific locus and features of genome erosion shared with other members of the tuberculosis complex."
Bentley S.D., Comas I., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., De Jong B.C.
Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GM041182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeq	YP_004724914.1. NC_015758.1.
3D structure databases
ProteinModelPortal	F8M6H6.
SMR	F8M6H6. Positions 12-227, 260-444.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC28343; CCC28343; MAF_32730.
GeneID	10957411.
KEGG	maf:MAF_32730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K12234.
Enzyme and pathway databases
BioCyc	MAFR572418:GJCK-3297-MONOMER.
UniPathway	UPA00071.
Family and domain databases
Gene3D	3.40.109.10. 1 hit.
HAMAP	MF_01259. F420_ligase_CofE_2.
InterPro	IPR002847. F420-0_gamma-glut_ligase-rel. IPR008225. F420-0_gamma-glutamyl_ligase. IPR019943. F420_FbiB_C. IPR023661. F420_ligase_CofE_2_bact. IPR000415. Nitroreductase-like. [Graphical view]
Pfam	PF01996. F420_ligase. 1 hit. PF00881. Nitroreductase. 1 hit. [Graphical view]
SUPFAM	SSF55469. Nitroreductase. 1 hit.
TIGRFAMs	TIGR01916. F420_cofE. 1 hit. TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F8M6H6_MYCA0

Accession

Primary (citable) accession number: F8M6H6

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information