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F8M6H6 (F8M6H6_MYCA0) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Coenzyme F420:L-glutamate ligase HAMAP-Rule MF_01259

EC=6.3.2.31 HAMAP-Rule MF_01259
EC=6.3.2.34 HAMAP-Rule MF_01259
Alternative name(s):
Coenzyme F420-0:L-glutamate ligase HAMAP-Rule MF_01259
Coenzyme F420-1:gamma-L-glutamate ligase HAMAP-Rule MF_01259
Gene names
Name:fbiB HAMAP-Rule MF_01259 EMBL CCC28343.1
Ordered Locus Names:MAF_32730 EMBL CCC28343.1
OrganismMycobacterium africanum (strain GM041182) [Complete proteome] [HAMAP] EMBL CCC28343.1
Taxonomic identifier572418 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives By similarity. HAMAP-Rule MF_01259

Catalytic activity

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1. HAMAP-Rule MF_01259

GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n. HAMAP-Rule MF_01259

GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2. HAMAP-Rule MF_01259

Cofactor

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese By similarity. HAMAP-Rule MF_01259

Monovalent cation. The ion could be potassium By similarity. HAMAP-Rule MF_01259

Pathway

Cofactor biosynthesis; coenzyme F420 biosynthesis. HAMAP-Rule MF_01259

Sequence similarities

In the N-terminal section; belongs to the CofE family. HAMAP-Rule MF_01259

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding20 – 234GTP By similarity HAMAP-Rule MF_01259
Region1 – 244244F420-0:gamma-glutamyl ligase By similarity HAMAP-Rule MF_01259
Region245 – 448204Unknown By similarity HAMAP-Rule MF_01259

Sites

Metal binding1091Divalent metal cation 1 By similarity HAMAP-Rule MF_01259
Metal binding1501Divalent metal cation 1 By similarity HAMAP-Rule MF_01259
Metal binding1511Divalent metal cation 2 By similarity HAMAP-Rule MF_01259
Binding site501GTP By similarity HAMAP-Rule MF_01259
Binding site551GTP By similarity HAMAP-Rule MF_01259
Binding site1121GTP By similarity HAMAP-Rule MF_01259

Sequences

Sequence LengthMass (Da)Tools
F8M6H6 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 8C6DB75DE6E86851

FASTA44847,578
        10         20         30         40         50         60 
MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS KVVSKCEGRL 

        70         80         90        100        110        120 
VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG LVQAAAGVDG SNVGRSELAL 

       130        140        150        160        170        180 
LPVDPDASAA TLRAGLRERL GVTVAVVITD TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR 

       190        200        210        220        230        240 
DPYGNELVVT EVAVADEIAA AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL 

       250        260        270        280        290        300 
FWLGTAEALE LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL 

       310        320        330        340        350        360 
QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP MLVPDGAHSY 

       370        380        390        400        410        420 
PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS TIFAADLVRD ELDLPVDWEP 

       430        440 
LGAIAIGYAD EPSGLRDPVP AADLLILK 

« Hide

References

[1]"The genome of Mycobacterium africanum West African 2 reveals a lineage-specific locus and genome erosion common to the M. tuberculosis complex."
Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
PLoS Negl. Trop. Dis. 6:E1552-E1552(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: GM041182 EMBL CCC28343.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeqYP_004724914.1. NC_015758.1.

3D structure databases

ProteinModelPortalF8M6H6.
SMRF8M6H6. Positions 12-227, 260-444.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC28343; CCC28343; MAF_32730.
GeneID10957411.
KEGGmaf:MAF_32730.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK12234.

Enzyme and pathway databases

BioCycMAFR572418:GJCK-3297-MONOMER.
UniPathwayUPA00071.

Family and domain databases

Gene3D3.40.109.10. 1 hit.
HAMAPMF_01259. F420_ligase_FbiB.
InterProIPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMSSF55469. SSF55469. 1 hit.
TIGRFAMsTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF8M6H6_MYCA0
AccessionPrimary (citable) accession number: F8M6H6
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)