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Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium africanum (strain GM041182)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K(+)UniRule annotationNote: Monovalent cation. The ion could be potassium.UniRule annotation
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501GTPUniRule annotation
Binding sitei55 – 551GTPUniRule annotation
Metal bindingi109 – 1091Divalent metal cation 1UniRule annotation
Binding sitei112 – 1121GTPUniRule annotation
Metal bindingi150 – 1501Divalent metal cation 1UniRule annotation
Metal bindingi151 – 1511Divalent metal cation 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 234GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMAFR572418:GJCK-3297-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligaseUniRule annotation
Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
Gene namesi
Name:fbiBUniRule annotationImported
Ordered Locus Names:MAF_32730Imported
OrganismiMycobacterium africanum (strain GM041182)Imported
Taxonomic identifieri572418 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000000298 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliF8M6H6.
SMRiF8M6H6. Positions 12-227, 260-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 244244F420-0:gamma-glutamyl ligaseUniRule annotationAdd
BLAST
Regioni245 – 448204UnknownUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CofE family.UniRule annotation

Phylogenomic databases

KOiK12234.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.

Sequencei

Sequence statusi: Complete.

F8M6H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS
60 70 80 90 100
KVVSKCEGRL VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG
110 120 130 140 150
LVQAAAGVDG SNVGRSELAL LPVDPDASAA TLRAGLRERL GVTVAVVITD
160 170 180 190 200
TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR DPYGNELVVT EVAVADEIAA
210 220 230 240 250
AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL FWLGTAEALE
260 270 280 290 300
LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL
310 320 330 340 350
QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP
360 370 380 390 400
MLVPDGAHSY PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS
410 420 430 440
TIFAADLVRD ELDLPVDWEP LGAIAIGYAD EPSGLRDPVP AADLLILK
Length:448
Mass (Da):47,578
Last modified:September 21, 2011 - v1
Checksum:i8C6DB75DE6E86851
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeqiWP_003900663.1. NC_015758.1.
YP_004724914.1. NC_015758.1.

Genome annotation databases

EnsemblBacteriaiCCC28343; CCC28343; MAF_32730.
KEGGimaf:MAF_32730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR878060 Genomic DNA. Translation: CCC28343.1.
RefSeqiWP_003900663.1. NC_015758.1.
YP_004724914.1. NC_015758.1.

3D structure databases

ProteinModelPortaliF8M6H6.
SMRiF8M6H6. Positions 12-227, 260-444.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC28343; CCC28343; MAF_32730.
KEGGimaf:MAF_32730.

Phylogenomic databases

KOiK12234.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMAFR572418:GJCK-3297-MONOMER.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Genome of Mycobacterium Africanum West African 2 Reveals a Lineage-Specific Locus and Genome Erosion Common to the M. tuberculosis Complex."
    Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
    PLoS Negl. Trop. Dis. 6:e1552-e1552(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: GM041182Imported.

Entry informationi

Entry nameiF8M6H6_MYCA0
AccessioniPrimary (citable) accession number: F8M6H6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: May 27, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.