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F8M6H6

- F8M6H6_MYCA0

UniProt

F8M6H6 - F8M6H6_MYCA0

Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium africanum (strain GM041182)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 24 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

    Catalytic activityi

    GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
    GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
    GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation
    Monovalent cation. The ion could be potassium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501GTPUniRule annotation
    Binding sitei55 – 551GTPUniRule annotation
    Metal bindingi109 – 1091Divalent metal cation 1UniRule annotation
    Binding sitei112 – 1121GTPUniRule annotation
    Metal bindingi150 – 1501Divalent metal cation 1UniRule annotation
    Metal bindingi151 – 1511Divalent metal cation 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 234GTPUniRule annotation

    GO - Molecular functioni

    1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
    2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
    3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
    2. F420-0 metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotation

    Keywords - Ligandi

    GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

    Enzyme and pathway databases

    BioCyciMAFR572418:GJCK-3297-MONOMER.
    UniPathwayiUPA00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
    Alternative name(s):
    Coenzyme F420-0:L-glutamate ligaseUniRule annotation
    Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
    Gene namesi
    Name:fbiBUniRule annotationImported
    Ordered Locus Names:MAF_32730Imported
    OrganismiMycobacterium africanum (strain GM041182)Imported
    Taxonomic identifieri572418 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000000298: Chromosome

    Structurei

    3D structure databases

    ProteinModelPortaliF8M6H6.
    SMRiF8M6H6. Positions 12-227, 260-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 244244F420-0:gamma-glutamyl ligaseUniRule annotationAdd
    BLAST
    Regioni245 – 448204UnknownUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the CofE family.UniRule annotation

    Phylogenomic databases

    KOiK12234.

    Family and domain databases

    Gene3Di3.40.109.10. 1 hit.
    HAMAPiMF_01259. F420_ligase_FbiB.
    InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR019943. F420_FbiB_C.
    IPR023661. F420_gamma-glut_ligase_bact.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF01996. F420_ligase. 1 hit.
    PF00881. Nitroreductase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55469. SSF55469. 1 hit.
    TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
    TIGR03553. F420_FbiB_CTERM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F8M6H6-1 [UniParc]FASTAAdd to Basket

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    MTGPEHGSAS TIEILPVIGL PEFRPGDDLS AAVAAAAPWL RDGDVVVVTS    50
    KVVSKCEGRL VPAPEDPEQR DRLRRKLIED EAVRVLARKD RTLITENRLG 100
    LVQAAAGVDG SNVGRSELAL LPVDPDASAA TLRAGLRERL GVTVAVVITD 150
    TMGRAWRNGQ TDAAVGAAGL AVLRNYAGVR DPYGNELVVT EVAVADEIAA 200
    AADLVKGKLT ATPVAVVRGF GVSDDGSTAR QLLRPGANDL FWLGTAEALE 250
    LGRQQAQLLR RSVRRFSTDP VPGDLVEAAV AEALTAPAPH HTRPTRFVWL 300
    QTPAIRARLL DRMKDKWRSD LTSDGLPADA IERRVARGQI LYDAPEVVIP 350
    MLVPDGAHSY PDAARTDAEH TMFTVAVGAA VQALLVALAV RGLGSCWIGS 400
    TIFAADLVRD ELDLPVDWEP LGAIAIGYAD EPSGLRDPVP AADLLILK 448
    Length:448
    Mass (Da):47,578
    Last modified:September 21, 2011 - v1
    Checksum:i8C6DB75DE6E86851
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR878060 Genomic DNA. Translation: CCC28343.1.
    RefSeqiYP_004724914.1. NC_015758.1.

    Genome annotation databases

    EnsemblBacteriaiCCC28343; CCC28343; MAF_32730.
    GeneIDi10957411.
    KEGGimaf:MAF_32730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR878060 Genomic DNA. Translation: CCC28343.1 .
    RefSeqi YP_004724914.1. NC_015758.1.

    3D structure databases

    ProteinModelPortali F8M6H6.
    SMRi F8M6H6. Positions 12-227, 260-444.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CCC28343 ; CCC28343 ; MAF_32730 .
    GeneIDi 10957411.
    KEGGi maf:MAF_32730.

    Phylogenomic databases

    KOi K12234.

    Enzyme and pathway databases

    UniPathwayi UPA00071 .
    BioCyci MAFR572418:GJCK-3297-MONOMER.

    Family and domain databases

    Gene3Di 3.40.109.10. 1 hit.
    HAMAPi MF_01259. F420_ligase_FbiB.
    InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR019943. F420_FbiB_C.
    IPR023661. F420_gamma-glut_ligase_bact.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view ]
    Pfami PF01996. F420_ligase. 1 hit.
    PF00881. Nitroreductase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55469. SSF55469. 1 hit.
    TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
    TIGR03553. F420_FbiB_CTERM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Mycobacterium africanum West African 2 reveals a lineage-specific locus and genome erosion common to the M. tuberculosis complex."
      Bentley S.D., Comas I., Bryant J.M., Walker D., Smith N.H., Harris S.R., Thurston S., Gagneux S., Wood J., Antonio M., Quail M.A., Gehre F., Adegbola R.A., Parkhill J., de Jong B.C.
      PLoS Negl. Trop. Dis. 6:E1552-E1552(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: GM041182Imported.

    Entry informationi

    Entry nameiF8M6H6_MYCA0
    AccessioniPrimary (citable) accession number: F8M6H6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 24 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3