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F8KAY7

- F8KAY7_9PSED

UniProt

F8KAY7 - F8KAY7_9PSED

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Protein
Submitted name:

Sec-alkylsulfatase

Gene

pisa1

Organism
Pseudomonas sp. DSM 6611
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Zinc 1; via tele nitrogenImported
Metal bindingi181 – 1811Zinc 1; via pros nitrogenImported
Metal bindingi183 – 1831Zinc 2Imported
Metal bindingi184 – 1841Zinc 2; via tele nitrogenImported
Metal bindingi291 – 2911Zinc 1Imported
Metal bindingi310 – 3101Zinc 1Imported
Metal bindingi310 – 3101Zinc 2Imported
Metal bindingi355 – 3551Zinc 2; via tele nitrogenImported

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Metal-bindingImported, ZincImported

Names & Taxonomyi

Protein namesi
Submitted name:
Sec-alkylsulfataseImported
Gene namesi
Name:pisa1Imported
OrganismiPseudomonas sp. DSM 6611Imported
Taxonomic identifieri1007495 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Interactioni

Protein-protein interaction databases

MINTiMINT-8397929.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YHEX-ray2.70A/B/C/D/E/F1-660[»]
4AV7X-ray3.00A/B/C/D/E/F1-660[»]
4AXHX-ray2.70A/B1-660[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.60.15.10. 2 hits.
InterProiIPR029229. Alkyl_sulf_C.
IPR029228. Alkyl_sulf_dimr.
IPR001279. Beta-lactamas-like.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamiPF14864. Alkyl_sulf_C. 1 hit.
PF14863. Alkyl_sulf_dimr. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF55718. SSF55718. 1 hit.
SSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

F8KAY7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRFIRASQR RTLLATLIAA TLAQPLLAAE SLDSKPASAI TAAKNAEVLK
60 70 80 90 100
NLPFADREEF EAAKRGLIAP FSGQIKNAEG QVVWDMGAYQ FLNDKDAADT
110 120 130 140 150
VNPSLWHQAQ LNNIAGLFEV MPKLYQVRGL DPANMTIIEG DSGLVLIDTL
160 170 180 190 200
TTAETARAAL DLYFQHRPKK PIVAVVYSHS HIDHFGGARG IIDEADVKAG
210 220 230 240 250
KVKVFAPSGF MEHAVSENIL AGTAMARRGQ YQSGVMVPRG AQAQVDSGLF
260 270 280 290 300
KTTATNATNT LVAPNVLIEK PYERHTVDGV ELEFQLTLGS EAPSDMNIYL
310 320 330 340 350
PQFKVLNTAD NAPPAMHNLL TPRGAEVRDA KAWAGYIDAS LEKYGDRTDV
360 370 380 390 400
LIQQHNWPVW GGDKVRTYLA DQRDMYAFLN NRALNLMNKG LTLHEIAAEV
410 420 430 440 450
SKLPGELDRK WYLRSYYGAL STNLRAVYQR YLGFYDGNPA NLDPFPPVEA
460 470 480 490 500
GKRYVEAMGG ADAVLKQMRA AIDKGDYRWA VQLGNHLVFA DPANKDARAL
510 520 530 540 550
QADAMEQLGY QTENALWRNM YMTGAMELRH GVPTYDSRGK SEMGRALTPD
560 570 580 590 600
MFFDLLAIRL DTDKAVGHDM TLNWVFEDLK QDIALTLRNG VLTQRVGSLN
610 620 630 640 650
PKADVTVKLT KPTLDQIAAR KLDLPTAIKQ GTVKLDGDGK KLGEFFGLLD
660
SFSPKFNIVE PLE
Length:663
Mass (Da):73,142
Last modified:September 21, 2011 - v1
Checksum:i682966688172CBA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR850678 Genomic DNA. Translation: CCA63329.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR850678 Genomic DNA. Translation: CCA63329.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YHE X-ray 2.70 A/B/C/D/E/F 1-660 [» ]
4AV7 X-ray 3.00 A/B/C/D/E/F 1-660 [» ]
4AXH X-ray 2.70 A/B 1-660 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8397929.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.1050.10. 1 hit.
3.60.15.10. 2 hits.
InterProi IPR029229. Alkyl_sulf_C.
IPR029228. Alkyl_sulf_dimr.
IPR001279. Beta-lactamas-like.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view ]
Pfami PF14864. Alkyl_sulf_C. 1 hit.
PF14863. Alkyl_sulf_dimr. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view ]
SMARTi SM00849. Lactamase_B. 1 hit.
[Graphical view ]
SUPFAMi SSF55718. SSF55718. 1 hit.
SSF56281. SSF56281. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A stereoselective inverting sec-alkylsulfatase for the deracemisation of sec-alcohols."
    Schober M., Gadler P., Knaus T., Kayer H., Birner-Gruenberger R., Guelly C., Macheroux P., Wagner U., Faber K.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 6611Imported.
  2. "Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates."
    Knaus T., Schober M., Kepplinger B., Faccinelli M., Pitzer J., Faber K., Macheroux P., Wagner U.
    FEBS J. 279:4374-4384(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 1-660 IN COMPLEX WITH ZINC.

Entry informationi

Entry nameiF8KAY7_9PSED
AccessioniPrimary (citable) accession number: F8KAY7
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3