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F8KAY7

- F8KAY7_9PSED

UniProt

F8KAY7 - F8KAY7_9PSED

Protein
Submitted name:

Sec-alkylsulfatase

Gene

pisa1

Organism
Pseudomonas sp. DSM 6611
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 15 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi179 – 1791Zinc 1; via tele nitrogenImported
    Metal bindingi181 – 1811Zinc 1; via pros nitrogenImported
    Metal bindingi183 – 1831Zinc 2Imported
    Metal bindingi184 – 1841Zinc 2; via tele nitrogenImported
    Metal bindingi291 – 2911Zinc 1Imported
    Metal bindingi310 – 3101Zinc 1Imported
    Metal bindingi310 – 3101Zinc 2Imported
    Metal bindingi355 – 3551Zinc 2; via tele nitrogenImported

    GO - Molecular functioni

    1. hydrolase activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Metal-bindingImported, ZincImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Sec-alkylsulfataseImported
    Gene namesi
    Name:pisa1Imported
    OrganismiPseudomonas sp. DSM 6611Imported
    Taxonomic identifieri1007495 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8397929.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YHEX-ray2.70A/B/C/D/E/F1-660[»]
    4AV7X-ray3.00A/B/C/D/E/F1-660[»]
    4AXHX-ray2.70A/B1-660[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di3.30.1050.10. 1 hit.
    3.60.15.10. 2 hits.
    InterProiIPR029229. Alkyl_sulf_C.
    IPR029228. Alkyl_sulf_dimr.
    IPR001279. Beta-lactamas-like.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view]
    PfamiPF14864. Alkyl_sulf_C. 1 hit.
    PF14863. Alkyl_sulf_dimr. 1 hit.
    PF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF55718. SSF55718. 1 hit.
    SSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F8KAY7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRFIRASQR RTLLATLIAA TLAQPLLAAE SLDSKPASAI TAAKNAEVLK    50
    NLPFADREEF EAAKRGLIAP FSGQIKNAEG QVVWDMGAYQ FLNDKDAADT 100
    VNPSLWHQAQ LNNIAGLFEV MPKLYQVRGL DPANMTIIEG DSGLVLIDTL 150
    TTAETARAAL DLYFQHRPKK PIVAVVYSHS HIDHFGGARG IIDEADVKAG 200
    KVKVFAPSGF MEHAVSENIL AGTAMARRGQ YQSGVMVPRG AQAQVDSGLF 250
    KTTATNATNT LVAPNVLIEK PYERHTVDGV ELEFQLTLGS EAPSDMNIYL 300
    PQFKVLNTAD NAPPAMHNLL TPRGAEVRDA KAWAGYIDAS LEKYGDRTDV 350
    LIQQHNWPVW GGDKVRTYLA DQRDMYAFLN NRALNLMNKG LTLHEIAAEV 400
    SKLPGELDRK WYLRSYYGAL STNLRAVYQR YLGFYDGNPA NLDPFPPVEA 450
    GKRYVEAMGG ADAVLKQMRA AIDKGDYRWA VQLGNHLVFA DPANKDARAL 500
    QADAMEQLGY QTENALWRNM YMTGAMELRH GVPTYDSRGK SEMGRALTPD 550
    MFFDLLAIRL DTDKAVGHDM TLNWVFEDLK QDIALTLRNG VLTQRVGSLN 600
    PKADVTVKLT KPTLDQIAAR KLDLPTAIKQ GTVKLDGDGK KLGEFFGLLD 650
    SFSPKFNIVE PLE 663
    Length:663
    Mass (Da):73,142
    Last modified:September 21, 2011 - v1
    Checksum:i682966688172CBA1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR850678 Genomic DNA. Translation: CCA63329.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR850678 Genomic DNA. Translation: CCA63329.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YHE X-ray 2.70 A/B/C/D/E/F 1-660 [» ]
    4AV7 X-ray 3.00 A/B/C/D/E/F 1-660 [» ]
    4AXH X-ray 2.70 A/B 1-660 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8397929.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.1050.10. 1 hit.
    3.60.15.10. 2 hits.
    InterProi IPR029229. Alkyl_sulf_C.
    IPR029228. Alkyl_sulf_dimr.
    IPR001279. Beta-lactamas-like.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view ]
    Pfami PF14864. Alkyl_sulf_C. 1 hit.
    PF14863. Alkyl_sulf_dimr. 1 hit.
    PF00753. Lactamase_B. 1 hit.
    [Graphical view ]
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55718. SSF55718. 1 hit.
    SSF56281. SSF56281. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A stereoselective inverting sec-alkylsulfatase for the deracemisation of sec-alcohols."
      Schober M., Gadler P., Knaus T., Kayer H., Birner-Gruenberger R., Guelly C., Macheroux P., Wagner U., Faber K.
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 6611Imported.
    2. "Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates."
      Knaus T., Schober M., Kepplinger B., Faccinelli M., Pitzer J., Faber K., Macheroux P., Wagner U.
      FEBS J. 279:4374-4384(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 1-660 IN COMPLEX WITH ZINC.

    Entry informationi

    Entry nameiF8KAY7_9PSED
    AccessioniPrimary (citable) accession number: F8KAY7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 15 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3