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F8JU34 (F8JU34_STREN) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable DNA ligase HAMAP-Rule MF_00407
EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:SCAT_0666 EMBL CCB73404.1
OrganismStreptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057) [Complete proteome] [HAMAP]
Taxonomic identifier1003195 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407 RuleBase RU000617

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407 RuleBase RU004196

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2101N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2081ATP By similarity HAMAP-Rule MF_00407
Binding site2151ATP By similarity HAMAP-Rule MF_00407
Binding site2301ATP By similarity HAMAP-Rule MF_00407
Binding site2591ATP By similarity HAMAP-Rule MF_00407
Binding site2991ATP By similarity HAMAP-Rule MF_00407
Binding site3771ATP By similarity HAMAP-Rule MF_00407
Binding site3831ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
F8JU34 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: CF33E3A1A3BF01F3

FASTA51755,999
        10         20         30         40         50         60 
MRFVEVARTS REVAATSARS RKISLLSGLF RDAEPSEAPV VITYLAGRLP QRRIGVGWST 

        70         80         90        100        110        120 
LSEPVPAAEE PRLAVLEVHL ALGRIASVVG QGAQHERRRL VHELLAAATE EEQRFLVALI 

       130        140        150        160        170        180 
AGELRQGALD ALAVEGLAAA VGVGAGDVRR AVMLRGSLGA VAEALLARGA EALTDFRLEV 

       190        200        210        220        230        240 
GRPVLPMLAH TARDVAEALD RLGPCAVEEK LDGIRVQVHR DGDLVRVYTR SLDEITDRLP 

       250        260        270        280        290        300 
EVVAEARALP GRQAVLDGEV IALGEDGRPR AFQETAGRVG SRLDVASAQA ALPVFPVFFD 

       310        320        330        340        350        360 
LLYVDGRDLL ELSTADRHAE LAALVPVPLR VRRIVAEDPG SPADRAAAEE FTEATLARGH 

       370        380        390        400        410        420 
EGVVLKALDA PYSAGRRGAS WLKVKPVHTL DLVVLAAEWG HGRRTGKLSN LHLGARRRNG 

       430        440        450        460        470        480 
TFAMLGKTFK GLTDAMLEWQ TERLQELTVH DERWGVVVRP ELVVEVAFDG VQRSPRYPEG 

       490        500        510 
VTLRFARVLR YREDKTPDQA DTVETVLGLR APAAPAP

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References

[1]"Complete genome sequence of Streptomyces cattleya NRRL 8057, a producer of antibiotics and fluorometabolites."
Barbe V., Bouzon M., Mangenot S., Badet B., Poulain J., Segurens B., Vallenet D., Marliere P., Weissenbach J.
J. Bacteriol. 193:5055-5056(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FQ859185 Genomic DNA. Translation: CCB73404.1.
RefSeqYP_004910211.1. NC_016111.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCB73404; CCB73404; SCAT_0666.
GeneID11356814.
KEGGsct:SCAT_0666.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01971.

Enzyme and pathway databases

BioCycSCAT1003195:GJCM-668-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF8JU34_STREN
AccessionPrimary (citable) accession number: F8JU34
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info