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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Sulfobacillus acidophilus (strain TPY)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei179UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei187UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei385Meso-diaminopimelateUniRule annotation1
Binding sitei461Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei465Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 116ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotationSAAS annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotationImported
Ordered Locus Names:TPY_3479Imported
OrganismiSulfobacillus acidophilus (strain TPY)Imported
Taxonomic identifieri1051632 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVII. Incertae SedisSulfobacillus
Proteomesi
  • UP000000291 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi1051632.TPY_3479.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 75Mur_ligaseInterPro annotationAdd BLAST53
Domaini108 – 313Mur_ligase_MInterPro annotationAdd BLAST206
Domaini334 – 419Mur_ligase_CInterPro annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 153UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni409 – 412Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi409 – 412Meso-diaminopimelate recognition motifUniRule annotation4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4107EEN. Bacteria.
COG0769. LUCA.
KOiK01928.
OMAiGRFNLYN.
OrthoDBiPOG091H0082.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

F8IAW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTINEFANIL SHAQVVGDGS VTVGGIQYDS RRVEKGDLFC AIPGFHTDGH
60 70 80 90 100
LYCQEAYQRG AVAFLVERAD TVPKGAPALI VPEARPAMAI VADAFYGHPS
110 120 130 140 150
GRLWMVGVTG TNGKTTTTHL IRAVLEGSDI PCGLTGTLHT LIGQETFKVV
160 170 180 190 200
RTTPEAPDLQ RILRHMADRG MRAAVMEVSS HALVLSRVDA VEYDVGVFTN
210 220 230 240 250
LTQDHLDFHH DIESYFRAKA LLFERLGTGR PKGPRAAVIN RDDPYSERLM
260 270 280 290 300
GMTGVPVLTY GIQQPSDIRA TQVNITSRGV QFVAEFPEGV SQPVEFGMPG
310 320 330 340 350
RFNVLNALAA MAVGYVYGLT PSEMAESLAR YPGVPGRFER IDEGQPFTVI
360 370 380 390 400
VDYAHTPDGL ENALKTAREF SLGKIGVVFG AGGDRDRGKR PLMGEVAGRL
410 420 430 440 450
ADWTVLTADN PRSEDPLDII HQIAEGVNRV GGQWQQELDR ERAIRLALNQ
460 470 480 490
AQPGDVVLIV GKGHETYQIY RDATIHFDDR EVARQILREL NKG
Length:493
Mass (Da):53,949
Last modified:September 21, 2011 - v1
Checksum:iCC7A207DFC9AB60E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002901 Genomic DNA. Translation: AEJ41631.1.

Genome annotation databases

EnsemblBacteriaiAEJ41631; AEJ41631; TPY_3479.
KEGGisay:TPY_3479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002901 Genomic DNA. Translation: AEJ41631.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1051632.TPY_3479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEJ41631; AEJ41631; TPY_3479.
KEGGisay:TPY_3479.

Phylogenomic databases

eggNOGiENOG4107EEN. Bacteria.
COG0769. LUCA.
KOiK01928.
OMAiGRFNLYN.
OrthoDBiPOG091H0082.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF8IAW5_SULAT
AccessioniPrimary (citable) accession number: F8IAW5
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.