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F8IAW5 (F8IAW5_SULAT) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208

EC=6.3.2.13 HAMAP-Rule MF_00208
Alternative name(s):
Meso-A2pm-adding enzyme HAMAP-Rule MF_00208
Meso-diaminopimelate-adding enzyme HAMAP-Rule MF_00208
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase HAMAP-Rule MF_00208
UDP-MurNAc-tripeptide synthetase HAMAP-Rule MF_00208
UDP-N-acetylmuramyl-tripeptide synthetase HAMAP-Rule MF_00208
Gene names
Name:murE HAMAP-Rule MF_00208 EMBL AEJ41631.1
Ordered Locus Names:TPY_3479 EMBL AEJ41631.1
OrganismSulfobacillus acidophilus (strain TPY) [Complete proteome] [HAMAP] EMBL AEJ41631.1
Taxonomic identifier1051632 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVII. Incertae SedisSulfobacillus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208 RuleBase RU004135

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00208 RuleBase RU004135 SAAS SAAS005761.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily. HAMAP-Rule MF_00208

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding110 – 1167ATP By similarity HAMAP-Rule MF_00208
Region152 – 1532UDP-MurNAc-L-Ala-D-Glu binding By similarity HAMAP-Rule MF_00208
Region409 – 4124Meso-diaminopimelate binding By similarity HAMAP-Rule MF_00208
Motif409 – 4124Meso-diaminopimelate recognition motif By similarity HAMAP-Rule MF_00208

Sites

Binding site301UDP-MurNAc-L-Ala-D-Glu By similarity HAMAP-Rule MF_00208
Binding site1791UDP-MurNAc-L-Ala-D-Glu By similarity HAMAP-Rule MF_00208
Binding site1871UDP-MurNAc-L-Ala-D-Glu By similarity HAMAP-Rule MF_00208
Binding site3851Meso-diaminopimelate By similarity HAMAP-Rule MF_00208
Binding site4611Meso-diaminopimelate; via carbonyl oxygen By similarity HAMAP-Rule MF_00208
Binding site4651Meso-diaminopimelate By similarity HAMAP-Rule MF_00208

Amino acid modifications

Modified residue2191N6-carboxylysine By similarity HAMAP-Rule MF_00208

Sequences

Sequence LengthMass (Da)Tools
F8IAW5 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: CC7A207DFC9AB60E

FASTA49353,949
        10         20         30         40         50         60 
MTINEFANIL SHAQVVGDGS VTVGGIQYDS RRVEKGDLFC AIPGFHTDGH LYCQEAYQRG 

        70         80         90        100        110        120 
AVAFLVERAD TVPKGAPALI VPEARPAMAI VADAFYGHPS GRLWMVGVTG TNGKTTTTHL 

       130        140        150        160        170        180 
IRAVLEGSDI PCGLTGTLHT LIGQETFKVV RTTPEAPDLQ RILRHMADRG MRAAVMEVSS 

       190        200        210        220        230        240 
HALVLSRVDA VEYDVGVFTN LTQDHLDFHH DIESYFRAKA LLFERLGTGR PKGPRAAVIN 

       250        260        270        280        290        300 
RDDPYSERLM GMTGVPVLTY GIQQPSDIRA TQVNITSRGV QFVAEFPEGV SQPVEFGMPG 

       310        320        330        340        350        360 
RFNVLNALAA MAVGYVYGLT PSEMAESLAR YPGVPGRFER IDEGQPFTVI VDYAHTPDGL 

       370        380        390        400        410        420 
ENALKTAREF SLGKIGVVFG AGGDRDRGKR PLMGEVAGRL ADWTVLTADN PRSEDPLDII 

       430        440        450        460        470        480 
HQIAEGVNRV GGQWQQELDR ERAIRLALNQ AQPGDVVLIV GKGHETYQIY RDATIHFDDR 

       490 
EVARQILREL NKG 

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References

[1]"Complete Genome Analysis of Sulfobacillus acidophilus Strain TPY, Isolated from a Hydrothermal Vent in the Pacific Ocean."
Li B., Chen Y., Liu Q., Hu S., Chen X.
J. Bacteriol. 193:5555-5556(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TPY EMBL AEJ41631.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002901 Genomic DNA. Translation: AEJ41631.1.
RefSeqYP_004721374.1. NC_015757.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEJ41631; AEJ41631; TPY_3479.
GeneID10962146.
KEGGsay:TPY_3479.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01928.
OMALFICIEG.

Enzyme and pathway databases

BioCycSACI1051632:GH78-3476-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00208. MurE.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF8IAW5_SULAT
AccessionPrimary (citable) accession number: F8IAW5
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)