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F8IAW5

- F8IAW5_SULAT

UniProt

F8IAW5 - F8IAW5_SULAT

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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Sulfobacillus acidophilus (strain TPY)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei179 – 1791UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei187 – 1871UDP-MurNAc-L-Ala-D-GluUniRule annotation
Binding sitei385 – 3851Meso-diaminopimelateUniRule annotation
Binding sitei461 – 4611Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
Binding sitei465 – 4651Meso-diaminopimelateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1167ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  5. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI1051632:GH78-3476-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotationImported
Ordered Locus Names:TPY_3479Imported
OrganismiSulfobacillus acidophilus (strain TPY)Imported
Taxonomic identifieri1051632 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVII. Incertae SedisSulfobacillus
ProteomesiUP000000291: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1532UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
Regioni409 – 4124Meso-diaminopimelate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi409 – 4124Meso-diaminopimelate recognition motifUniRule annotation

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

KOiK01928.
OMAiLFICIEG.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

F8IAW5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTINEFANIL SHAQVVGDGS VTVGGIQYDS RRVEKGDLFC AIPGFHTDGH
60 70 80 90 100
LYCQEAYQRG AVAFLVERAD TVPKGAPALI VPEARPAMAI VADAFYGHPS
110 120 130 140 150
GRLWMVGVTG TNGKTTTTHL IRAVLEGSDI PCGLTGTLHT LIGQETFKVV
160 170 180 190 200
RTTPEAPDLQ RILRHMADRG MRAAVMEVSS HALVLSRVDA VEYDVGVFTN
210 220 230 240 250
LTQDHLDFHH DIESYFRAKA LLFERLGTGR PKGPRAAVIN RDDPYSERLM
260 270 280 290 300
GMTGVPVLTY GIQQPSDIRA TQVNITSRGV QFVAEFPEGV SQPVEFGMPG
310 320 330 340 350
RFNVLNALAA MAVGYVYGLT PSEMAESLAR YPGVPGRFER IDEGQPFTVI
360 370 380 390 400
VDYAHTPDGL ENALKTAREF SLGKIGVVFG AGGDRDRGKR PLMGEVAGRL
410 420 430 440 450
ADWTVLTADN PRSEDPLDII HQIAEGVNRV GGQWQQELDR ERAIRLALNQ
460 470 480 490
AQPGDVVLIV GKGHETYQIY RDATIHFDDR EVARQILREL NKG
Length:493
Mass (Da):53,949
Last modified:September 21, 2011 - v1
Checksum:iCC7A207DFC9AB60E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002901 Genomic DNA. Translation: AEJ41631.1.
RefSeqiYP_004721374.1. NC_015757.1.

Genome annotation databases

EnsemblBacteriaiAEJ41631; AEJ41631; TPY_3479.
GeneIDi10962146.
KEGGisay:TPY_3479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002901 Genomic DNA. Translation: AEJ41631.1 .
RefSeqi YP_004721374.1. NC_015757.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEJ41631 ; AEJ41631 ; TPY_3479 .
GeneIDi 10962146.
KEGGi say:TPY_3479.

Phylogenomic databases

KOi K01928.
OMAi LFICIEG.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci SACI1051632:GH78-3476-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPi MF_00208. MurE.
InterProi IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view ]
Pfami PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view ]
SUPFAMi SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsi TIGR01085. murE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome analysis of Sulfobacillus acidophilus strain TPY, isolated from a hydrothermal vent in the Pacific ocean."
    Li B., Chen Y., Liu Q., Hu S., Chen X.
    J. Bacteriol. 193:5555-5556(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TPYImported.

Entry informationi

Entry nameiF8IAW5_SULAT
AccessioniPrimary (citable) accession number: F8IAW5
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3