Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

F8IAW5

- F8IAW5_SULAT

UniProt

F8IAW5 - F8IAW5_SULAT

Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Sulfobacillus acidophilus (strain TPY)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

    Catalytic activityi

    ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei179 – 1791UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei187 – 1871UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei385 – 3851Meso-diaminopimelateUniRule annotation
    Binding sitei461 – 4611Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
    Binding sitei465 – 4651Meso-diaminopimelateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1167ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    4. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    LigaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Cell cycle, Cell divisionUniRule annotationSAAS annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSACI1051632:GH78-3476-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
    Alternative name(s):
    Meso-A2pm-adding enzymeUniRule annotation
    Meso-diaminopimelate-adding enzymeUniRule annotation
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
    UDP-MurNAc-tripeptide synthetaseUniRule annotation
    UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
    Gene namesi
    Name:murEUniRule annotationImported
    Ordered Locus Names:TPY_3479Imported
    OrganismiSulfobacillus acidophilus (strain TPY)Imported
    Taxonomic identifieri1051632 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVII. Incertae SedisSulfobacillus
    ProteomesiUP000000291: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei219 – 2191N6-carboxylysineUniRule annotation

    Post-translational modificationi

    Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni152 – 1532UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
    Regioni409 – 4124Meso-diaminopimelate bindingUniRule annotation

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi409 – 4124Meso-diaminopimelate recognition motifUniRule annotation

    Sequence similaritiesi

    Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01928.
    OMAiLFICIEG.

    Family and domain databases

    Gene3Di3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPiMF_00208. MurE.
    InterProiIPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view]
    PfamiPF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view]
    SUPFAMiSSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsiTIGR01085. murE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F8IAW5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTINEFANIL SHAQVVGDGS VTVGGIQYDS RRVEKGDLFC AIPGFHTDGH    50
    LYCQEAYQRG AVAFLVERAD TVPKGAPALI VPEARPAMAI VADAFYGHPS 100
    GRLWMVGVTG TNGKTTTTHL IRAVLEGSDI PCGLTGTLHT LIGQETFKVV 150
    RTTPEAPDLQ RILRHMADRG MRAAVMEVSS HALVLSRVDA VEYDVGVFTN 200
    LTQDHLDFHH DIESYFRAKA LLFERLGTGR PKGPRAAVIN RDDPYSERLM 250
    GMTGVPVLTY GIQQPSDIRA TQVNITSRGV QFVAEFPEGV SQPVEFGMPG 300
    RFNVLNALAA MAVGYVYGLT PSEMAESLAR YPGVPGRFER IDEGQPFTVI 350
    VDYAHTPDGL ENALKTAREF SLGKIGVVFG AGGDRDRGKR PLMGEVAGRL 400
    ADWTVLTADN PRSEDPLDII HQIAEGVNRV GGQWQQELDR ERAIRLALNQ 450
    AQPGDVVLIV GKGHETYQIY RDATIHFDDR EVARQILREL NKG 493
    Length:493
    Mass (Da):53,949
    Last modified:September 21, 2011 - v1
    Checksum:iCC7A207DFC9AB60E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002901 Genomic DNA. Translation: AEJ41631.1.
    RefSeqiWP_013988345.1. NC_015757.1.
    YP_004721374.1. NC_015757.1.

    Genome annotation databases

    EnsemblBacteriaiAEJ41631; AEJ41631; TPY_3479.
    GeneIDi10962146.
    KEGGisay:TPY_3479.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002901 Genomic DNA. Translation: AEJ41631.1 .
    RefSeqi WP_013988345.1. NC_015757.1.
    YP_004721374.1. NC_015757.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEJ41631 ; AEJ41631 ; TPY_3479 .
    GeneIDi 10962146.
    KEGGi say:TPY_3479.

    Phylogenomic databases

    KOi K01928.
    OMAi LFICIEG.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci SACI1051632:GH78-3476-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPi MF_00208. MurE.
    InterProi IPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view ]
    Pfami PF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsi TIGR01085. murE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome analysis of Sulfobacillus acidophilus strain TPY, isolated from a hydrothermal vent in the Pacific ocean."
      Li B., Chen Y., Liu Q., Hu S., Chen X.
      J. Bacteriol. 193:5555-5556(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TPYImported.

    Entry informationi

    Entry nameiF8IAW5_SULAT
    AccessioniPrimary (citable) accession number: F8IAW5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3