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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Weissella koreensis (strain KACC 15510)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:WKK_03905Imported
OrganismiWeissella koreensis (strain KACC 15510)Imported
Taxonomic identifieri1045854 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeWeissella
Proteomesi
  • UP000008930 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi1045854.WKK_03905.

Structurei

3D structure databases

ProteinModelPortaliF8I1A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini571 – 674TRNA-bindingInterPro annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 24"HIGH" regionUniRule annotationAdd BLAST11
Motifi309 – 313"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
KOiK01874.
OMAiIYPEMLV.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PfamiView protein in Pfam
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.

Sequencei

Sequence statusi: Complete.

F8I1A4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKKTFYIT TPIYYPSGKL HIGNTYTTVL ADAAARYHRL LNEDVYYLTG
60 70 80 90 100
TDEHGLKIEQ KADKMGVTPQ AYVDSMADDI KKLWASLDIT NDGFIRTTDE
110 120 130 140 150
IHEKAVTKIF NQLLEQGDIY KGEYEGWYSV DDEEYFTESQ LAEAYRDESG
160 170 180 190 200
KVIGGKAPSG HEVELVKEES YFFAMSKYAD WLLEYYQAHP DFIQPVTRMN
210 220 230 240 250
EMINNFIKPG LEDLAVTRTS FKWGVPVESD PKHVIYVWID ALSNYITALG
260 270 280 290 300
YDSEDDSKFK KYWPANVQLL GKEIVRFHTI YWPIMLHALG LELPENVIGH
310 320 330 340 350
GWLTMKDGKM SKSKGNVVYP DVLADRYGVD AVRYYLLRAM PFGNDGIFTP
360 370 380 390 400
EDFVGKLNSD LANDLGNLLN RTVAMINKYE DGVIPTLETG ATEFDENLIE
410 420 430 440 450
ITDEVIAKYN ENMQILHVSD ALAEVWKLIS RANKYIDETQ PWVLAKNEEN
460 470 480 490 500
QSILASVMAH LAASLRVIAI LLQPALSRTP AEIFKQLGLS KSNLAIDQLK
510 520 530 540 550
FSDLPTGGKV ISKGTPIFPR IDVEKEVTYI RDSMMGGGKE TASVKKAKNA
560 570 580 590 600
ESKVQNVDQK EIKPNFIEFD DFEKVSLKVA EIKNVSEIEN SKKLLKFELD
610 620 630 640 650
DGTAKGRTIL SGIKKWYPEY QQLIGKKVAF VANLKPRTMM KKYVSEGMLL
660 670
SAEKDGNVTL SILPDEIEAG SELG
Length:674
Mass (Da):76,105
Last modified:September 21, 2011 - v1
Checksum:iA205B4441EA55BF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002899 Genomic DNA. Translation: AEJ23655.1.
RefSeqiWP_013989533.1. NC_015759.1.

Genome annotation databases

EnsemblBacteriaiAEJ23655; AEJ23655; WKK_03905.
KEGGiwko:WKK_03905.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002899 Genomic DNA. Translation: AEJ23655.1.
RefSeqiWP_013989533.1. NC_015759.1.

3D structure databases

ProteinModelPortaliF8I1A4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1045854.WKK_03905.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEJ23655; AEJ23655; WKK_03905.
KEGGiwko:WKK_03905.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
KOiK01874.
OMAiIYPEMLV.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PfamiView protein in Pfam
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF8I1A4_WEIKK
AccessioniPrimary (citable) accession number: F8I1A4
Entry historyiIntegrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: February 15, 2017
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.