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F8I1A4 (F8I1A4_WEIKK) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228

EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228
Ordered Locus Names:WKK_03905 EMBL AEJ23655.1
OrganismWeissella koreensis (strain KACC 15510) [Complete proteome] [HAMAP] EMBL AEJ23655.1
Taxonomic identifier1045854 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeWeissella

Protein attributes

Sequence length674 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228 SAAS SAAS023457

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228 SAAS SAAS023457

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228 SAAS SAAS014758.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. HAMAP-Rule MF_01228

Contains 1 tRNA-binding domain. HAMAP-Rule MF_01228

Contains tRNA-binding domain. SAAS SAAS014758

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain571 – 674104tRNA-binding By similarity HAMAP-Rule MF_01228
Motif14 – 2411"HIGH" region By similarity HAMAP-Rule MF_01228
Motif309 – 3135"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1611Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
F8I1A4 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: A205B4441EA55BF4

FASTA67476,105
        10         20         30         40         50         60 
MTDKKTFYIT TPIYYPSGKL HIGNTYTTVL ADAAARYHRL LNEDVYYLTG TDEHGLKIEQ 

        70         80         90        100        110        120 
KADKMGVTPQ AYVDSMADDI KKLWASLDIT NDGFIRTTDE IHEKAVTKIF NQLLEQGDIY 

       130        140        150        160        170        180 
KGEYEGWYSV DDEEYFTESQ LAEAYRDESG KVIGGKAPSG HEVELVKEES YFFAMSKYAD 

       190        200        210        220        230        240 
WLLEYYQAHP DFIQPVTRMN EMINNFIKPG LEDLAVTRTS FKWGVPVESD PKHVIYVWID 

       250        260        270        280        290        300 
ALSNYITALG YDSEDDSKFK KYWPANVQLL GKEIVRFHTI YWPIMLHALG LELPENVIGH 

       310        320        330        340        350        360 
GWLTMKDGKM SKSKGNVVYP DVLADRYGVD AVRYYLLRAM PFGNDGIFTP EDFVGKLNSD 

       370        380        390        400        410        420 
LANDLGNLLN RTVAMINKYE DGVIPTLETG ATEFDENLIE ITDEVIAKYN ENMQILHVSD 

       430        440        450        460        470        480 
ALAEVWKLIS RANKYIDETQ PWVLAKNEEN QSILASVMAH LAASLRVIAI LLQPALSRTP 

       490        500        510        520        530        540 
AEIFKQLGLS KSNLAIDQLK FSDLPTGGKV ISKGTPIFPR IDVEKEVTYI RDSMMGGGKE 

       550        560        570        580        590        600 
TASVKKAKNA ESKVQNVDQK EIKPNFIEFD DFEKVSLKVA EIKNVSEIEN SKKLLKFELD 

       610        620        630        640        650        660 
DGTAKGRTIL SGIKKWYPEY QQLIGKKVAF VANLKPRTMM KKYVSEGMLL SAEKDGNVTL 

       670 
SILPDEIEAG SELG 

« Hide

References

[1]"Complete Genome Sequence of Weissella koreensis KACC 15510, Isolated from Kimchi."
Lee S.H., Jung J.Y., Lee S.H., Jeon C.O.
J. Bacteriol. 193:5534-5534(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KACC 15510 EMBL AEJ23655.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002899 Genomic DNA. Translation: AEJ23655.1.
RefSeqYP_004726334.1. NC_015759.1.

3D structure databases

ProteinModelPortalF8I1A4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEJ23655; AEJ23655; WKK_03905.
GeneID10954076.
KEGGwko:WKK_03905.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01874.
OMAQVCVVAN.

Enzyme and pathway databases

BioCycWKOR1045854:GH3T-801-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF8I1A4_WEIKK
AccessionPrimary (citable) accession number: F8I1A4
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)