Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine--tRNA ligase

Gene

metG

Organism
Weissella koreensis (strain KACC 15510)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi161 – 1611ZincUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciWKOR1045854:GH3T-801-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.10UniRule annotationSAAS annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:WKK_03905Imported
OrganismiWeissella koreensis (strain KACC 15510)Imported
Taxonomic identifieri1045854 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeWeissella
ProteomesiUP000008930: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF8I1A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini571 – 674104tRNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi14 – 2411"HIGH" regionUniRule annotationAdd
BLAST
Motifi309 – 3135"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation
Contains tRNA-binding domain.SAAS annotation

Phylogenomic databases

KOiK01874.
OMAiDKVLCPD.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8I1A4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKKTFYIT TPIYYPSGKL HIGNTYTTVL ADAAARYHRL LNEDVYYLTG
60 70 80 90 100
TDEHGLKIEQ KADKMGVTPQ AYVDSMADDI KKLWASLDIT NDGFIRTTDE
110 120 130 140 150
IHEKAVTKIF NQLLEQGDIY KGEYEGWYSV DDEEYFTESQ LAEAYRDESG
160 170 180 190 200
KVIGGKAPSG HEVELVKEES YFFAMSKYAD WLLEYYQAHP DFIQPVTRMN
210 220 230 240 250
EMINNFIKPG LEDLAVTRTS FKWGVPVESD PKHVIYVWID ALSNYITALG
260 270 280 290 300
YDSEDDSKFK KYWPANVQLL GKEIVRFHTI YWPIMLHALG LELPENVIGH
310 320 330 340 350
GWLTMKDGKM SKSKGNVVYP DVLADRYGVD AVRYYLLRAM PFGNDGIFTP
360 370 380 390 400
EDFVGKLNSD LANDLGNLLN RTVAMINKYE DGVIPTLETG ATEFDENLIE
410 420 430 440 450
ITDEVIAKYN ENMQILHVSD ALAEVWKLIS RANKYIDETQ PWVLAKNEEN
460 470 480 490 500
QSILASVMAH LAASLRVIAI LLQPALSRTP AEIFKQLGLS KSNLAIDQLK
510 520 530 540 550
FSDLPTGGKV ISKGTPIFPR IDVEKEVTYI RDSMMGGGKE TASVKKAKNA
560 570 580 590 600
ESKVQNVDQK EIKPNFIEFD DFEKVSLKVA EIKNVSEIEN SKKLLKFELD
610 620 630 640 650
DGTAKGRTIL SGIKKWYPEY QQLIGKKVAF VANLKPRTMM KKYVSEGMLL
660 670
SAEKDGNVTL SILPDEIEAG SELG
Length:674
Mass (Da):76,105
Last modified:September 21, 2011 - v1
Checksum:iA205B4441EA55BF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002899 Genomic DNA. Translation: AEJ23655.1.
RefSeqiWP_013989533.1. NC_015759.1.
YP_004726334.1. NC_015759.1.

Genome annotation databases

EnsemblBacteriaiAEJ23655; AEJ23655; WKK_03905.
GeneIDi10954076.
KEGGiwko:WKK_03905.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002899 Genomic DNA. Translation: AEJ23655.1.
RefSeqiWP_013989533.1. NC_015759.1.
YP_004726334.1. NC_015759.1.

3D structure databases

ProteinModelPortaliF8I1A4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEJ23655; AEJ23655; WKK_03905.
GeneIDi10954076.
KEGGiwko:WKK_03905.

Phylogenomic databases

KOiK01874.
OMAiDKVLCPD.

Enzyme and pathway databases

BioCyciWKOR1045854:GH3T-801-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Weissella koreensis KACC 15510, isolated from Kimchi."
    Lee S.H., Jung J.Y., Lee S.H., Jeon C.O.
    J. Bacteriol. 193:5534-5534(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KACC 15510Imported.

Entry informationi

Entry nameiF8I1A4_WEIKK
AccessioniPrimary (citable) accession number: F8I1A4
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: February 4, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.