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F8GNE1

- F8GNE1_CUPNN

UniProt

F8GNE1 - F8GNE1_CUPNN

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Protein
Ribulose bisphosphate carboxylase large chain
Gene
cbbL, CNE_2c13530
Organism
Cupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261Substrate; in homodimeric partner By similarityUniRule annotation
Binding sitei176 – 1761Substrate By similarityUniRule annotation
Active sitei178 – 1781Proton acceptor By similarityUniRule annotation
Binding sitei180 – 1801Substrate By similarityUniRule annotation
Metal bindingi204 – 2041Magnesium; via carbamate group By similarityUniRule annotation
Metal bindingi206 – 2061Magnesium By similarityUniRule annotation
Metal bindingi207 – 2071Magnesium By similarityUniRule annotation
Active sitei296 – 2961Proton acceptor By similarityUniRule annotation
Binding sitei297 – 2971Substrate By similarityUniRule annotation
Binding sitei329 – 3291Substrate By similarityUniRule annotation
Sitei336 – 3361Transition state stabilizer By similarityUniRule annotation
Binding sitei381 – 3811Substrate By similarityUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciCNEC1042878:GH0Z-5042-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotationImported
Ordered Locus Names:CNE_2c13530Imported
OrganismiCupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)Imported
Taxonomic identifieri1042878 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000006798: Chromosome 2

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei204 – 2041N6-carboxylysine By similarityUniRule annotation

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains By similarity.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8GNE1-1 [UniParc]FASTAAdd to Basket

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MNAPESVQAK PRKRYDAGVM KYKEMGYWDG DYEPKDTDLL ALFRITPQDG    50
VDPVEAAAAV AGESSTATWT VVWTDRLTAC DMYRAKAYRV DPVPNNPEQF 100
FCYVAYDLSL FEEGSIANLT ASIIGNVFSF KPIKAARLED MRFPVAYVKT 150
FAGPSTGIIV ERERLDKFGR PLLGATTKPK LGLSGRNYGR VVYEGLKGGL 200
DFMKDDENIN SQPFMHWRDR FLFVMDAVNK ASAATGEVKG SYLNVTAGTM 250
EEMYRRAEFA KSLGSVIIMI DLIVGWTCIQ SMSNWCRQND VILHLHRAGH 300
GTYTRQKNHG VSFRVIAKWL RLAGVDHMHT GTAVGKLEGD PLTVQGYYNV 350
CRDAYTHTDL SRGLFFDQDW ASLRKVMPVA SGGIHAGQMH QLIHLFGDDV 400
VLQFGGGTIG HPQGIQAGAT ANRVALEAMV LARNEGRDIL NEGPEILRDA 450
ARWCGPLRAA LDTWGDISFN YTPTDTSDFA PTASVA 486
Length:486
Mass (Da):53,777
Last modified:September 21, 2011 - v1
Checksum:i315108F9B1EC21CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002878 Genomic DNA. Translation: AEI80316.1.
RefSeqiWP_013953010.1. NC_015723.1.
YP_004681548.1. NC_015723.1.

Genome annotation databases

EnsemblBacteriaiAEI80316; AEI80316; CNE_2c13530.
GeneIDi10921831.
KEGGicnc:CNE_2c13530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002878 Genomic DNA. Translation: AEI80316.1 .
RefSeqi WP_013953010.1. NC_015723.1.
YP_004681548.1. NC_015723.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEI80316 ; AEI80316 ; CNE_2c13530 .
GeneIDi 10921831.
KEGGi cnc:CNE_2c13530.

Phylogenomic databases

KOi K01601.

Enzyme and pathway databases

BioCyci CNEC1042878:GH0Z-5042-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPi MF_01338. RuBisCO_L_type1.
InterProi IPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete Genome Sequence of the Type Strain Cupriavidus necator N-1."
    Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B.
    J. Bacteriol. 193:5017-5017(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43291 / DSM 13513 / N-1.

Entry informationi

Entry nameiF8GNE1_CUPNN
AccessioniPrimary (citable) accession number: F8GNE1
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: September 3, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi