F8GLK7 (F8GLK7_NITSI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121
Short name=Asp/Glu-ADT subunit B HAMAP-Rule MF_00121
EC=6.3.5.- HAMAP-Rule MF_00121

Gene names

Name:	gatB HAMAP-Rule MF_00121
Ordered Locus Names:	Nit79A3_0883 EMBL AEJ00753.1

Organism

Nitrosomonas sp. (strain Is79A3) [Complete proteome] [HAMAP]

Taxonomic identifier

261292 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosomonas

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121 SAAS SAAS018027
Catalytic activity	ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121 SAAS SAAS018027 ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00121 SAAS SAAS018027
Sequence similarities	Belongs to the gatB/gatE family. GatB subfamily. HAMAP-Rule MF_00121

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00121
Ligand	ATP-binding HAMAP-Rule MF_00121 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00121 Transferase EMBL AEJ00753.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	F8GLK7 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 5F05C223BB0AB9B6	FASTA	479	52,765
	10 20 30 40 50 60 MQWEIVIGLE VHAQLSTQSK IFSGSSTAYG AAPNTQASVI DLALPGVLPV LNKGAVERAI 70 80 90 100 110 120 KLGLSVGAKI NSPSIFARKN YFYPDLPKGY QISQYELPVV QGGNIQIQID GIEKNVRLTR 130 140 150 160 170 180 AHLEEDAGKS LHEDFHGMSG IDLNRAGTPL LEIVSEPDMR SSAEAVAYAK TLHALVRWIG 190 200 210 220 230 240 ICDGNMQEGS FRCDANVSVR PRGTEKLGTR CEIKNLNSFR FLEKAIDYEA RRQIEILEDG 250 260 270 280 290 300 GAIRQETRLY DANKDETRTM RTKEDANDYR YFPDPDLLPL EIPQSWIDQI KQTLPELPQA 310 320 330 340 350 360 RRDRYISEFA LSAYDASVLT SSREMADYFD ATLKQLPAQA KLCANWIMGE ISGQLNKEGI 370 380 390 400 410 420 EMTACPITPA QLAALLARIS DGTISGKAAK TVFESMWNGE LDKNVDAIIE AKGLKQISDD 430 440 450 460 470 GAIEKLVDQV LAANAQQVAD YRNGKEKAFN SLIGQIMKAT QGKANPAQVN AMLKKKLDG

References

[1]

"Complete sequence of Nitrosomonas sp. Is79A3." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Bollmann A. , Norton J., Suwa Y., Klotz M., Stein L., Laanbroek H., Arp D., Woyke T. Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Is79A3 EMBL AEJ00753.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002876 Genomic DNA. Translation: AEJ00753.1.
RefSeq	YP_004694152.1. NC_015731.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEJ00753; AEJ00753; Nit79A3_0883.
GeneID	10932972.
KEGG	nii:Nit79A3_0883.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K02434.
Enzyme and pathway databases
BioCyc	NSP261292:GH7H-900-MONOMER.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F8GLK7_NITSI

Accession

Primary (citable) accession number: F8GLK7

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)