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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Nitrosomonas sp. (strain Is79A3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301Substrate; in homodimeric partnerUniRule annotation
Binding sitei180 – 1801SubstrateUniRule annotation
Active sitei182 – 1821Proton acceptorUniRule annotation
Binding sitei184 – 1841SubstrateUniRule annotation
Metal bindingi208 – 2081Magnesium; via carbamate groupUniRule annotation
Metal bindingi210 – 2101MagnesiumUniRule annotation
Metal bindingi211 – 2111MagnesiumUniRule annotation
Active sitei300 – 3001Proton acceptorUniRule annotation
Binding sitei301 – 3011SubstrateUniRule annotation
Binding sitei333 – 3331SubstrateUniRule annotation
Sitei340 – 3401Transition state stabilizerUniRule annotation
Binding sitei385 – 3851SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciNSP261292:GH7H-3270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Ordered Locus Names:Nit79A3_3235Imported
OrganismiNitrosomonas sp. (strain Is79A3)Imported
Taxonomic identifieri261292 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000000501 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei208 – 2081N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Protein-protein interaction databases

STRINGi261292.Nit79A3_3235.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiQACDAYL.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8GL95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASETMKEGK ERYKSGVIPY KKMGYWEPSY VPKDTDVIAL FRITPQPGVD
60 70 80 90 100
HEEAAAAVAG ESSTATWTVV WTDRLTACEL YRAKAYKSEL VPNTGPGTKN
110 120 130 140 150
EAQYFAYIAY DIDLFEEGSI ANLTASIIGN VFGFKAVKAL RLEDMRIPVA
160 170 180 190 200
YLKTFQGPAT GIVVERERLD KFGRPLLGAT TKPKLGLSGR NYGRVVYEGL
210 220 230 240 250
KGGLDFMKDD ENINSQPFMH WRDRFLYCME AVNKASAATG EVKGHYLNVT
260 270 280 290 300
AGTMEDMYER AEFAKSLGSV IVMIDLVIGY TAIQSMAKWA RRNDMILHLH
310 320 330 340 350
RAGNSTYSRQ KNHGMNFRVI CKWMRMAGVD HIHAGTVVGK LEGDPLMIKG
360 370 380 390 400
FYDTLRDTHT EKNLEHGLFF DQDWASLNKV MPVASGGIHA GQMHQLLDYL
410 420 430 440 450
GEDVILQFGG GTIGHPQGIQ AGATANRVAL EAMVLARNEG RDYVKEGPQI
460 470 480 490
LADAAKWCTP LKQALDTWKD ITFNYDSTDT ADFVPSATAN V
Length:491
Mass (Da):54,472
Last modified:September 21, 2011 - v1
Checksum:iE1561A3351780AF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002876 Genomic DNA. Translation: AEJ02969.1.
RefSeqiWP_013967179.1. NC_015731.1.

Genome annotation databases

EnsemblBacteriaiAEJ02969; AEJ02969; Nit79A3_3235.
KEGGinii:Nit79A3_3235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002876 Genomic DNA. Translation: AEJ02969.1.
RefSeqiWP_013967179.1. NC_015731.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi261292.Nit79A3_3235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEJ02969; AEJ02969; Nit79A3_3235.
KEGGinii:Nit79A3_3235.

Phylogenomic databases

KOiK01601.
OMAiQACDAYL.

Enzyme and pathway databases

BioCyciNSP261292:GH7H-3270-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Is79A3Imported.

Entry informationi

Entry nameiF8GL95_NITSI
AccessioniPrimary (citable) accession number: F8GL95
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: July 22, 2015
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.