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F8GE44 (F8GE44_NITSI) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Nit79A3_3539 EMBL AEJ03260.1
OrganismNitrosomonas sp. (strain Is79A3) [Complete proteome] [HAMAP] EMBL AEJ03260.1
Taxonomic identifier261292 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain105 – 300196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding131 – 18656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2541Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2671Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2671Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F8GE44 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 2189A7EB2513542D

FASTA30533,691
        10         20         30         40         50         60 
MITHNFGKVA VLLGGRSAER EISLQSGQAV LDALRRSGVD AYPFDPAEQA LEELVHQDFK 

        70         80         90        100        110        120 
RAFIALHGRF GEDGTIQGVL EWLGLPYTGS GVMASALAMD KWRTKLIWQA TGIQSPRYEL 

       130        140        150        160        170        180 
LQADSNFEQV AETLGLPLIV KPAREGSTIG LSKVHHKQDL AEAYRLAAQH DALVLAEEFI 

       190        200        210        220        230        240 
AGTELTVAIL GETSLPVVRI EIAGELYDYQ AKYFSNETKY FCPSGLPDEL ESIIQNQALR 

       250        260        270        280        290        300 
AYQVLGCQGW GRVDLILSQT NQFYFLEANT SPGMTNHSLV PMAARTARIS FEDLVLRILD 


LSHVE 

« Hide

References

[1]"Complete sequence of Nitrosomonas sp. Is79A3."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Bollmann A. expand/collapse author list , Norton J., Suwa Y., Klotz M., Stein L., Laanbroek H., Arp D., Woyke T.
Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Is79A3 EMBL AEJ03260.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002876 Genomic DNA. Translation: AEJ03260.1.
RefSeqYP_004696659.1. NC_015731.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEJ03260; AEJ03260; Nit79A3_3539.
GeneID10935647.
KEGGnii:Nit79A3_3539.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycNSP261292:GH7H-3576-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF8GE44_NITSI
AccessionPrimary (citable) accession number: F8GE44
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: February 19, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)