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F8G642 (F8G642_PSEPU) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
EC=2.4.1.18 HAMAP-Rule MF_00685
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP-Rule MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP-Rule MF_00685
Glycogen branching enzyme HAMAP-Rule MF_00685
Gene names
Name:glgB HAMAP-Rule MF_00685
ORF Names:PPS_3463 EMBL AEJ14010.1
OrganismPseudomonas putida S16 EMBL AEJ14010.1
Taxonomic identifier1042876 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685 SAAS SAAS006048

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685 SAAS SAAS006048

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP-Rule MF_00685

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site4171Nucleophile By similarity HAMAP-Rule MF_00685
Active site4701Proton donor By similarity HAMAP-Rule MF_00685

Sequences

Sequence LengthMass (Da)Tools
F8G642 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 95E6B23FB1D6ADEB

FASTA73682,882
        10         20         30         40         50         60 
MNATTRENGG LRQRDLDALA RAEHADPFAV LGPHGDGAGG QVVRAFLPNA LNVRILARHD 

        70         80         90        100        110        120 
GRILAEMEQG SLPGLFTAHL DEAHPYLLQI GWAGGEQITE DPYSFGPQLG DMDLYLFAEG 

       130        140        150        160        170        180 
NHRDLSGRFG AQPIQVDGVD GVCFSVWAPN ARRVSVVGDF NNWDGRRHPM RLRHSAGVWE 

       190        200        210        220        230        240 
LFVPRLGVGE TYKFEVLGKD GILPLKADPL ARATELPPST ASKVAGALSH DWQDHDWMAQ 

       250        260        270        280        290        300 
RAQRHAYSAP LSIYELHPGS WRCELDEAGE VGRYYNWREL AERLVPYVQE LGFTHIELLP 

       310        320        330        340        350        360 
IMEHPFGGSW GYQPLSLFAP TSRYGSAEDF AAFIDACHQG GIGVLLDWVP AHFPTDEHGL 

       370        380        390        400        410        420 
ARFDGTALYE YDNPLEGYHQ DWNTLIYNLG RNEVRGFMMA SALHWLKHFH IDGLRVDAVA 

       430        440        450        460        470        480 
SMLYRDYSRK AGEWVPNRHG GRENLEAIDF IRHLNGVAAH EAPGALIIAE ESTAWPGVSQ 

       490        500        510        520        530        540 
PTQQGGLGFA YKWNMGWMHD TLHYIQNDPV HRTYHHNEMS FGLIYAYSEH FILPISHDEV 

       550        560        570        580        590        600 
VHGKHSLIDK MPGDRWQKFA NLRAYLTFMW AHPGKKLLFM GCEFGQWREW DHDGELDWYL 

       610        620        630        640        650        660 
LQYPEHQGVQ RLVGDLNRLY REVPALHEQD CQPQGFQWLI GDDAQNSVYA WLRWSSSGEP 

       670        680        690        700        710        720 
VLVVANFTPV PREGYRIGVP FGERWQELLN SDAELYAGSN VGNLGVVESD QIASHGQPQS 

       730 
LALNLPPLGV LIMKPA

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References

[1]"Complete Genome Sequence of the Nicotine-Degrading Pseudomonas putida Strain S16."
Yu H., Tang H., Wang L., Yao Y., Wu G., Xu P.
J. Bacteriol. 193:5541-5542(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: S16 EMBL AEJ14010.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002870 Genomic DNA. Translation: AEJ14010.1.
RefSeqYP_004702890.1. NC_015733.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEJ14010; AEJ14010; PPS_3463.
GeneID10939093.
KEGGppt:PPS_3463.

Phylogenomic databases

KOK00700.

Enzyme and pathway databases

BioCycPPUT1042876:GH0R-3421-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006407. 1-4-A-glucan_branch_enz.
IPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PTHR10357:SF13. PTHR10357:SF13. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
SSF81296. Ig_E-set. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF8G642_PSEPU
AccessionPrimary (citable) accession number: F8G642
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info