ID   F8F2M7_GRAC1            Unreviewed;       452 AA.
AC   F8F2M7;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   27-MAR-2024, entry version 53.
DE   SubName: Full=Hexokinase {ECO:0000313|EMBL:AEJ19142.1};
DE            EC=2.7.1.1 {ECO:0000313|EMBL:AEJ19142.1};
GN   OrderedLocusNames=Spica_0992 {ECO:0000313|EMBL:AEJ19142.1};
OS   Gracilinema caldarium (strain ATCC 51460 / DSM 7334 / H1) (Treponema
OS   caldarium).
OC   Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Breznakiellaceae;
OC   Gracilinema.
OX   NCBI_TaxID=744872 {ECO:0000313|EMBL:AEJ19142.1, ECO:0000313|Proteomes:UP000000503};
RN   [1] {ECO:0000313|Proteomes:UP000000503}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 51460 / DSM 7334 / H1 {ECO:0000313|Proteomes:UP000000503};
RX   PubMed=23961314; DOI=10.4056/sigs.3096473;
RA   Abt B., Goker M., Scheuner C., Han C., Lu M., Misra M., Lapidus A.,
RA   Nolan M., Lucas S., Hammon N., Deshpande S., Cheng J.F., Tapia R.,
RA   Goodwin L.A., Pitluck S., Liolios K., Pagani I., Ivanova N., Mavromatis K.,
RA   Mikhailova N., Huntemann M., Pati A., Chen A., Palaniappan K., Land M.,
RA   Hauser L., Jeffries C.D., Rohde M., Spring S., Gronow S., Detter J.C.,
RA   Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C.,
RA   Woyke T., Klenk H.P.;
RT   "Genome sequence of the thermophilic fresh-water bacterium Spirochaeta
RT   caldaria type strain (H1(T)), reclassification of Spirochaeta caldaria,
RT   Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema
RT   as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and
RT   Treponema zuelzerae comb. nov., and emendation of the genus Treponema.";
RL   Stand. Genomic Sci. 8:88-105(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001397};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC         Evidence={ECO:0000256|ARBA:ARBA00001397};
CC   -!- PATHWAY: Carbohydrate degradation. {ECO:0000256|ARBA:ARBA00004921}.
CC   -!- PATHWAY: Carbohydrate metabolism. {ECO:0000256|ARBA:ARBA00005007}.
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000256|ARBA:ARBA00009225}.
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DR   EMBL; CP002868; AEJ19142.1; -; Genomic_DNA.
DR   RefSeq; WP_013968453.1; NC_015732.1.
DR   AlphaFoldDB; F8F2M7; -.
DR   STRING; 744872.Spica_0992; -.
DR   KEGG; scd:Spica_0992; -.
DR   eggNOG; COG5026; Bacteria.
DR   HOGENOM; CLU_014393_5_3_12; -.
DR   OrthoDB; 6383434at2; -.
DR   UniPathway; UPA00109; UER00180.
DR   Proteomes; UP000000503; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:RHEA.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR   Gene3D; 3.30.420.40; -; 1.
DR   Gene3D; 3.40.367.20; -; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; HEXOKINASE; 1.
DR   PANTHER; PTHR19443:SF16; HEXOKINASE TYPE 1-RELATED; 1.
DR   Pfam; PF00349; Hexokinase_1; 1.
DR   Pfam; PF03727; Hexokinase_2; 1.
DR   PRINTS; PR00475; HEXOKINASE.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR   PROSITE; PS51748; HEXOKINASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000503};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AEJ19142.1}.
FT   DOMAIN          29..200
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          225..450
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
SQ   SEQUENCE   452 AA;  48502 MW;  0C261F97F6FA62F6 CRC64;
     MHSSFDPRLL ADFARYYGFH YGVCDPVALV KDLVIEMERG LRGDPSTLPM LPSYLYPVSA
     VPPGKAVIAL DAGGTNLRAA LVRFDEKGKA IAEHTQKTHM PGTKGQLKAQ QFFDEIAEVT
     TPLLKENPHV EGIGFCFSYP MEMTKDADGI LLGFSKEVDA PEVIGKAIGA GLREALARKG
     VKAPDRIVLL NDTVATLLSG LAEIPADGGH RKGPDIYGVE GGPVIGFILG TGMNVAYPET
     RIPKIGFDAP KSPQIVVCET GSFHPRYLGR LDEEFDATLK NPGKYTFEKT MAGAYLGPLT
     LYMLKKAVQD GVLVFRKSGE LLAMQNLQTK DLNAFMHNPL AGEGPIGSLF DRDERDALAT
     LVFLTSIITE RAALFAAAAV AAAVEKTGAG YDPFVPVRVA VEGTTYMVYK GMRPALESYL
     HTILSAKAPR SIVVSPVEQA SLFGAAVAAL SK
//