Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Zymomonas mobilis subsp. pomaceae (strain ATCC 29192 / JCM 10191 / NBRC 13757 / NCIMB 11200 / NRRL B-4491)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPUniRule annotation1
Binding sitei147ATPUniRule annotation1
Binding sitei192ATPUniRule annotation1
Binding sitei215ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 158ATPUniRule annotation7
Nucleotide bindingi184 – 187ATPUniRule annotation4
Nucleotide bindingi269 – 270ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation, LyaseImported
Biological processPurine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotation
Ordered Locus Names:Zymop_1645Imported
OrganismiZymomonas mobilis subsp. pomaceae (strain ATCC 29192 / JCM 10191 / NBRC 13757 / NCIMB 11200 / NRRL B-4491)Imported
Taxonomic identifieri579138 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas
Proteomesi
  • UP000000491 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi579138.Zymop_1645.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 299ATP-graspInterPro annotationAdd BLAST189

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CY8. Bacteria.
COG0026. LUCA.
KOiK01589.
OMAiAPRTHNS.
OrthoDBiPOG091H069C.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR016185. PreATP-grasp_dom_sf.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
PfamiView protein in Pfam
PF02222. ATP-grasp. 1 hit.
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

F8EWC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTFPPGSK IGIIGNGQLG QMMAIAAIQL GYFCHIYAPN QHGPANIVAA
60 70 80 90 100
KSFVGDYGDS DKLNEFAATV DVLTYEFENI PAEPLIDLSK QMPVYPPPRA
110 120 130 140 150
LQIAQDRLVE KNYAAEQGIR PAPFRAVNSA KELSYALLEL GVPAILKQRT
160 170 180 190 200
QGYDGRGQFP VKKMTDAHLA EIWNSLSVKQ AVLEGMVRFE YEFSVIAVRN
210 220 230 240 250
ADGQFLTWDV PVNTHNKGIL AHSVVPANEF IQQQADAAIK ATHKIADSLG
260 270 280 290 300
YVGVLAVEFF ATQFGPIFNE MAPRVHNSGH WTIEGSETSQ FENHIRAICG
310 320 330 340 350
LPLGSTALTA EKVEMKNLIG TQINTWEKIL SHKNEYLHIY GKGTPRPGRK
360
MGHYTKLSRR IF
Length:362
Mass (Da):40,022
Last modified:September 21, 2011 - v1
Checksum:i31465C539901EFFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002865 Genomic DNA. Translation: AEI38534.1.
RefSeqiWP_013934922.1. NC_015709.1.

Genome annotation databases

EnsemblBacteriaiAEI38534; AEI38534; Zymop_1645.
KEGGizmp:Zymop_1645.
PATRICifig|579138.3.peg.1747.

Similar proteinsi

Entry informationi

Entry nameiF8EWC4_ZYMMT
AccessioniPrimary (citable) accession number: F8EWC4
Entry historyiIntegrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: November 22, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported