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Protein
Submitted name:

D-alanine--D-alanine ligase

Gene

Runsl_3158

Organism
Runella slithyformis (strain ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.SAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.SAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.SAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseSAAS annotationImported

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationSAAS annotation, Peptidoglycan synthesisSAAS annotation

Keywords - Ligandi

ATP-bindingSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSLI761193:GHKZ-3181-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Submitted name:
D-alanine--D-alanine ligaseImported (EC:6.3.2.4Imported)
Gene namesi
Ordered Locus Names:Runsl_3158Imported
OrganismiRunella slithyformis (strain ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4)Imported
Taxonomic identifieri761193 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesCytophagaceaeRunella
Proteomesi
  • UP000000493 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi761193.Runsl_3158.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 435211ATP-graspInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.SAAS annotation
Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
KOiK01921.
OMAiFKQMAEI.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF07478. Dala_Dala_lig_C. 2 hits.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8EN42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGIFFGGP AREREISYAG GKTAYENMDK ALFTPVLVFV DSLGNFILTD
60 70 80 90 100
ESTLYHSGIR AFYPGPAFRE GGFEVYIESL QQQLSPAELE ALMHGIGTPI
110 120 130 140 150
QPQDFKKYFD FAFIILHGPD CEDGAIQGLM EWHKMPYMGP GLLGSAVSID
160 170 180 190 200
KILQNEQIAR ANGQEKKMAV VRWEEWHGAD ISQKFEEAKA AVGLPIVVKA
210 220 230 240 250
PHQGSSIGVA IVKEDSADAF AKAVNQCFFS LEIRADDWRS WSEAEKHTFA
260 270 280 290 300
QRIGNLDESI GYPVVIQETQ ETIYHPLDLL DKLDASLHSS FTLLSINAED
310 320 330 340 350
QVLLEEFMTG QEFSCGVIQD NDGTVIALPP TEIAKMDENQ TFDFKTKYKL
360 370 380 390 400
NVTRKFIPVR TALENNQKIQ YNIALVFEKL GMNAVARIDG FLTPDGRVLL
410 420 430 440 450
HDPNTLPGMS PTSLIFKQMA EIGLDVTHAI TYLIRQSLRE RIRTGKDTIH
460 470 480 490 500
LRKLLSTLDA DIAQKLAERT LQNVEFEATT EAYMEARRTF SQLSASGKVK
510 520 530 540 550
PTAVLKISDD EKHELPVGLL FKDTIEDVLE MVAQPVHPLI TETREKAKNI

TRRFVG
Length:556
Mass (Da):62,244
Last modified:September 21, 2011 - v1
Checksum:i4E44F7725A539D38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002859 Genomic DNA. Translation: AEI49539.1.
RefSeqiWP_013928846.1. NC_015703.1.

Genome annotation databases

EnsemblBacteriaiAEI49539; AEI49539; Runsl_3158.
KEGGirsi:Runsl_3158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002859 Genomic DNA. Translation: AEI49539.1.
RefSeqiWP_013928846.1. NC_015703.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi761193.Runsl_3158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI49539; AEI49539; Runsl_3158.
KEGGirsi:Runsl_3158.

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
KOiK01921.
OMAiFKQMAEI.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciRSLI761193:GHKZ-3181-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF07478. Dala_Dala_lig_C. 2 hits.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4Imported.

Entry informationi

Entry nameiF8EN42_RUNSL
AccessioniPrimary (citable) accession number: F8EN42
Entry historyi
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: July 6, 2016
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.