F8EN42 (F8EN42_RUNSL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
D-alanine--D-alanine ligase EMBL AEI49539.1
EC=6.3.2.4 EMBL AEI49539.1

Gene names

Ordered Locus Names:

Runsl_3158 EMBL AEI49539.1

Organism

Runella slithyformis (strain ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4) [Complete proteome] [HAMAP]

Taxonomic identifier

761193 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Cytophagia › Cytophagales › Cytophagaceae › Runella › Runella slithyformis

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS005905
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905
Subcellular location	Cytoplasm By similarity SAAS SAAS005905.
Sequence similarities	Contains 1 ATP-grasp domain. SAAS SAAS005905

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS005905 Peptidoglycan synthesis SAAS SAAS005905
Cellular component	Cytoplasm SAAS SAAS005905
Ligand	ATP-binding SAAS SAAS005905 Magnesium SAAS SAAS005905 Manganese SAAS SAAS005905 Metal-binding SAAS SAAS005905 Nucleotide-binding
Molecular function	Ligase SAAS SAAS005905 EMBL AEI49539.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	F8EN42 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 4E44F7725A539D38	FASTA	556	62,244
	10 20 30 40 50 60 MRIGIFFGGP AREREISYAG GKTAYENMDK ALFTPVLVFV DSLGNFILTD ESTLYHSGIR 70 80 90 100 110 120 AFYPGPAFRE GGFEVYIESL QQQLSPAELE ALMHGIGTPI QPQDFKKYFD FAFIILHGPD 130 140 150 160 170 180 CEDGAIQGLM EWHKMPYMGP GLLGSAVSID KILQNEQIAR ANGQEKKMAV VRWEEWHGAD 190 200 210 220 230 240 ISQKFEEAKA AVGLPIVVKA PHQGSSIGVA IVKEDSADAF AKAVNQCFFS LEIRADDWRS 250 260 270 280 290 300 WSEAEKHTFA QRIGNLDESI GYPVVIQETQ ETIYHPLDLL DKLDASLHSS FTLLSINAED 310 320 330 340 350 360 QVLLEEFMTG QEFSCGVIQD NDGTVIALPP TEIAKMDENQ TFDFKTKYKL NVTRKFIPVR 370 380 390 400 410 420 TALENNQKIQ YNIALVFEKL GMNAVARIDG FLTPDGRVLL HDPNTLPGMS PTSLIFKQMA 430 440 450 460 470 480 EIGLDVTHAI TYLIRQSLRE RIRTGKDTIH LRKLLSTLDA DIAQKLAERT LQNVEFEATT 490 500 510 520 530 540 EAYMEARRTF SQLSASGKVK PTAVLKISDD EKHELPVGLL FKDTIEDVLE MVAQPVHPLI 550 TETREKAKNI TRRFVG

References

[1]

"The complete genome of chromosome of Runella slithyformis DSM 19594." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Han J., Lapidus A., Bruce D., Goodwin L., Pitluck S., Peters L., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G., Zhang X., Misra M., Detter J.C., Tapia R., Han C., Land M., Hauser L. , Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindall B., Faehrich R., Brambilla E., Klenk H.-P., Eisen J.A. Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002859 Genomic DNA. Translation: AEI49539.1.
RefSeq	YP_004656671.1. NC_015703.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEI49539; AEI49539; Runsl_3158.
GeneID	10871690.
KEGG	rsi:Runsl_3158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01921.
Enzyme and pathway databases
BioCyc	RSLI761193:GHKZ-3318-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 2 hits. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F8EN42_RUNSL

Accession

Primary (citable) accession number: F8EN42

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)