F8DT27 (INVB_ZYMMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular sucrase
EC=3.2.1.26

Alternative name(s):
Beta-fructofuranosidase
Invertase
Protein B46
Saccharase

Gene names

Name:	sacC
Synonyms:	invB, sucE3
Ordered Locus Names:	Zmob_0915

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) [Complete proteome] [HAMAP]

Taxonomic identifier

555217 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Sequence similarities	Belongs to the glycosyl hydrolase 68 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate utilization Inferred from electronic annotation. Source: InterPro
Molecular_function	levansucrase activity Inferred from electronic annotation. Source: InterPro sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Extracellular sucrase		PRO_0000414234
Experimental info
	Sequence conflict	85	1	G → A in AAC36942. Ref.2
	Sequence conflict	404 – 407	4	PVWP → LGMA in AAC36942. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	F8DT27 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: BACC12D167332E47	FASTA	413	46,100
	10 20 30 40 50 60 MFNFNASRWT RAQAMKVNKF DLTTSMPEIG TDFPIMRDDL WLWDTWPLRD INGNPVSFKG 70 80 90 100 110 120 WNVIFSLVAD RNIPWNDRHS HARIGYFYSK DGKSWVYGGH LLQESANTRT AEWSGGTIMA 130 140 150 160 170 180 PGSRNQVETF FTSTLFDKNG VREAVAAVTK GRIYADSEGV WFKGFDQSTD LFQADGLFYQ 190 200 210 220 230 240 NYAENNLWNF RDPHVFINPE DGETYALFEA NVATVRGEDD IGEDEIGPVP ANTVVPKDAN 250 260 270 280 290 300 LCSASIGIAR CLSPDRTEWE LLPPLLTAFG VNDQMERPHV IFQNGLTYLF TISHDSTYAD 310 320 330 340 350 360 GLTGSDGLYG FVSENGIFGP YEPLNGSGLV LGGPASQPTE AYAHYIMNNG LVESFINEII 370 380 390 400 410 DPKSGKVIAG GSLAPTVRVE LQGHETFATE VFDYGYIPAS YAWPVWPFPD RRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide and derived amino acid sequences of an extracellular sucrase gene (invB) of Zymomonas mobilis ZM1 (ATCC10988)." Song K.B., Lee S.K., Joo H.K., Rhee S.-K. Biochim. Biophys. Acta 1219:163-166(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1.
[2]	"Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis." Kannan R., Mukundan G., Ait-Abdelkader N., Augier-Magro V., Baratti J., Gunasekaran P. Arch. Microbiol. 163:195-204(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1.
[3]	"Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis lectotype strain ATCC 10988." Pappas K.M., Kouvelis V.N., Saunders E., Brettin T.S., Bruce D., Detter C., Balakireva M., Han C.S., Savvakis G., Kyrpides N.C., Typas M.A. J. Bacteriol. 193:5051-5052(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081588 Genomic DNA. Translation: AAA61488.1. L33403 Genomic DNA. Translation: AAC36942.1. CP002850 Genomic DNA. Translation: AEH62750.1.
PIR	JC2520. S47527.
RefSeq	YP_005621201.1. NC_017262.1.
3D structure databases
ProteinModelPortal	F8DT27.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEH62750; AEH62750; Zmob_0915.
GeneID	12280849.
KEGG	zmm:Zmob_0915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00692.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR003469. Glyco_hydro_68. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF02435. Glyco_hydro_68. 1 hit. [Graphical view]
SUPFAM	SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INVB_ZYMMA

Accession

Primary (citable) accession number: F8DT27
Secondary accession number(s): Q5NQK5 , Q60115, Q60117, Q60125

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 14, 2011
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program