F8DIY5 (F8DIY5_STREP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ribonuclease 3 HAMAP-Rule MF_00104
EC=3.1.26.3 HAMAP-Rule MF_00104

Alternative name(s):
Ribonuclease III HAMAP-Rule MF_00104

Gene names

Name:	rnc HAMAP-Rule MF_00104
Ordered Locus Names:	HMPREF0833_10900 EMBL AEH55931.1

Organism

Streptococcus parasanguinis (strain ATCC 15912 / DSM 6778 / CIP 104372 / LMG 14537) [Complete proteome] [HAMAP]

Taxonomic identifier

760570 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus parasanguis

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104 SAAS SAAS001159
Cofactor	Mg2+ By similarity. HAMAP-Rule MF_00104 SAAS SAAS000999
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00104 SAAS SAAS001159
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00104 SAAS SAAS001159.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. HAMAP-Rule MF_00104 SAAS SAAS001159 Contains 1 RNase III domain. HAMAP-Rule MF_00104 SAAS SAAS001159

Ontologies

Keywords
Biological process	mRNA processing HAMAP-Rule MF_00104 SAAS SAAS001159 rRNA processing SAAS SAAS001159 HAMAP-Rule MF_00104 tRNA processing SAAS SAAS001159 HAMAP-Rule MF_00104
Cellular component	Cytoplasm HAMAP-Rule MF_00104 SAAS SAAS001159
Ligand	Magnesium SAAS SAAS001159 HAMAP-Rule MF_00104 Metal-binding SAAS SAAS001159 HAMAP-Rule MF_00104 RNA-binding rRNA-binding SAAS SAAS000999 HAMAP-Rule MF_00104
Molecular function	Endonuclease SAAS SAAS001159 HAMAP-Rule MF_00104 Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	5 – 134	130	RNase III By similarity HAMAP-Rule MF_00104
	Domain	160 – 229	70	DRBM By similarity HAMAP-Rule MF_00104
Sites
	Active site	51	1	By similarity HAMAP-Rule MF_00104
	Active site	123	1	By similarity HAMAP-Rule MF_00104
	Metal binding	47	1	Magnesium By similarity HAMAP-Rule MF_00104
	Metal binding	120	1	Magnesium By similarity HAMAP-Rule MF_00104
	Metal binding	123	1	Magnesium By similarity HAMAP-Rule MF_00104

Sequences

	Sequence		Length	Mass (Da)
	F8DIY5 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 865844B31B9D1450	FASTA	233	26,509
	10 20 30 40 50 60 MEKLQALLLE RFQIVFSDSS LLDTAFTHTS YANEHRLLKI SHNERLEFLG DAVLQLVISE 70 80 90 100 110 120 YLYKEHPNRP EGDLSKFRSM IVREESLAGF SRDCQFDRYI KLGKGEEKSG GRDRDTILGD 130 140 150 160 170 180 LFEAFLGALL LDQGVEAVKR FIYQVMIPKV ETGQFSQVID YKTRLQELLQ VHGDVEIQYQ 190 200 210 220 230 VVSESGPAHA KEFEVEVFVN GQTMGHGHGR SKKLAEQEAA RNAVETRNDS PCI

References

[1]

"Complete sequence of Streptococcus parasanguinis strain ATCC 15912." Muzny D., Qin X., Buhay C., Dugan-Rocha S., Ding Y., Chen G., Hawes A., Holder M., Jhangiani S., Johnson A., Khan Z., Li Z., Liu W., Liu X., Perez L., Shen H., Wang Q., Watt J. , Xi L., Xin Y., Zhou J., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L., Villasana D., Johnson A., Liu J., Liyanage D., Lorensuhewa L., Robinson T., Song A., Song B.-B., Dinh H., Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V., Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G., Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A., Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C., Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S., Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S., Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L., Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P., Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K., Petrosino J., Highlander S., Gibbs R. Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15912 / DSM 6778 / CIP 104372 / LMG 14537.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002843 Genomic DNA. Translation: AEH55931.1.
RefSeq	YP_004621859.1. NC_015678.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEH55931; AEH55931; HMPREF0833_10900.
GeneID	10835399.
KEGG	scp:HMPREF0833_10900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K03685.
Enzyme and pathway databases
BioCyc	SPAR760570:GI5E-921-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F8DIY5_STREP

Accession

Primary (citable) accession number: F8DIY5

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	May 29, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)