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F8CN61 (F8CN61_MYXFH) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA(Ile)-lysidine synthase HAMAP-Rule MF_01161
EC=6.3.4.19 HAMAP-Rule MF_01161
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase HAMAP-Rule MF_01161
tRNA(Ile)-lysidine synthetase HAMAP-Rule MF_01161
Gene names
Name:tilS HAMAP-Rule MF_01161
Ordered Locus Names:LILAB_29930 EMBL AEI67867.1
OrganismMyxococcus fulvus (strain ATCC BAA-855 / HW-1) [Complete proteome] [HAMAP] EMBL AEI67867.1
Taxonomic identifier483219 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus ›

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. SAAS SAAS012796 HAMAP-Rule MF_01161

Catalytic activity

(tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O. HAMAP-Rule MF_01161 SAAS SAAS012796

Subcellular location

Cytoplasm By similarity SAAS SAAS012796 HAMAP-Rule MF_01161.

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding By similarity. HAMAP-Rule MF_01161

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family. HAMAP-Rule MF_01161

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding6 – 116ATP By similarity HAMAP-Rule MF_01161

Sequences

Sequence LengthMass (Da)Tools
F8CN61 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 73C1692D1111A12E

FASTA42444,741
        10         20         30         40         50         60 
MLLAVSGGAD STALLVGTAC VAQALRLRVE VATLDHGLRP ESTGEARSVA LLAATRGLPC 

        70         80         90        100        110        120 
HVRALGLRPG AGVEARAREA RYAALEALRR ESGLDAVATA HTATDQAETL LMRLARGTAL 

       130        140        150        160        170        180 
RGAVGIHEAR PGLVRPLLAC TRQDVEAFVA EQGVTYSTDP MNADPVHFRT RVRRDVLPAL 

       190        200        210        220        230        240 
SRAAGFAVEA HLAAFARVAA EDEALLASLA DAAFERLRLE DGALDAVGVR ALVPALRRRV 

       250        260        270        280        290        300 
LARLVAGADA AVDEATLARV QRAVERGGTA TLGRGALLRA SSGRVRCVRQ TSSPRPPAEL 

       310        320        330        340        350        360 
RLERAGARGA LIGTGWNFSV ESAPPPSGVH GLALEDGTRW PLTVRTRRPG DRVRTPAGQR 

       370        380        390        400        410        420 
KLQDVLVDLR VPAEARATRP VVADAEGQVV WLPGLWPPAV PRAAAREYLW AVPPGPSIQR 


TAAL

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References

[1]"Genome Sequence of the Halotolerant Marine Bacterium Myxococcus fulvus HW-1."
Li Z.F., Li X., Liu H., Liu X., Han K., Wu Z.H., Hu W., Li F.F., Li Y.Z.
J. Bacteriol. 193:5015-5016(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-855 / HW-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002830 Genomic DNA. Translation: AEI67867.1.
RefSeqYP_004668945.1. NC_015711.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEI67867; AEI67867; LILAB_29930.
GeneID10880450.
KEGGmfu:LILAB_29930.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK04075.
OMARERECAK.

Enzyme and pathway databases

BioCycMFUL483219:GJEO-5987-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_01161. tRNA_Ile__lys_synt.
InterProIPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamPF01171. ATP_bind_3. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
TIGRFAMsTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF8CN61_MYXFH
AccessionPrimary (citable) accession number: F8CN61
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: May 29, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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