Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

F8AM17

- F8AM17_METOI

UniProt

F8AM17 - F8AM17_METOI

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanothermococcus okinawensis (strain DSM 14208 / JCM 11175 / IH1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 21 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531Proton acceptorUniRule annotation
    Binding sitei155 – 1551SubstrateUniRule annotation
    Metal bindingi179 – 1791Magnesium; via carbamate groupUniRule annotation
    Metal bindingi181 – 1811MagnesiumUniRule annotation
    Metal bindingi182 – 1821MagnesiumUniRule annotation
    Active sitei269 – 2691Proton acceptorUniRule annotation
    Binding sitei270 – 2701SubstrateUniRule annotation
    Binding sitei302 – 3021SubstrateUniRule annotation
    Sitei309 – 3091Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: InterPro

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMOKI647113:GHB9-744-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Metok_0719Imported
    OrganismiMethanothermococcus okinawensis (strain DSM 14208 / JCM 11175 / IH1)Imported
    Taxonomic identifieri647113 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanothermococcus
    ProteomesiUP000009296: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei179 – 1791N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliF8AM17.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni353 – 3553Substrate bindingUniRule annotation
    Regioni375 – 3784Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family.UniRule annotation
    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F8AM17-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYLELGYEP KDNELLSCMV VKAENLKKAA NDIAGESSIG TWTELKTMKS    50
    EIYKKLKPRV YEINEMGKEG DTNIGLIKIA YPLDTFEPNN MPGTLAGIAG 100
    NIFGMKSLNG LRILDFRFPK EFIKCYSGPK YGIKGIRKLL NVMERPLVGT 150
    IVKPKVGLNS EEHAKVAYNS WIGGCDIVKD DENLVSQDFN KFEGRLHKVL 200
    ECRDKAESET GEKKIYMPNI TAPYREMVRR AELVEDAGCE YAMIDVVVLG 250
    FSAVQQIREE GFNFAIHAHR AMHAAITRSR EWGISMLALA KIYRLLGVDQ 300
    LHIGTVVGKM EGGKDEVCSI RDEIVLEDVK EDNKNKFFNQ KWHGLNNTFP 350
    VSSGGVYPKL VPEIVNIFGK DVIIQAGGGI HGHPEGTVAG ARAMRASVEA 400
    SIEGIPLSEK AEEVSELKKA LEYWK 425
    Length:425
    Mass (Da):47,362
    Last modified:September 21, 2011 - v1
    Checksum:iE2A1A4B6BF3F5829
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002792 Genomic DNA. Translation: AEH06696.1.
    RefSeqiWP_013866882.1. NC_015636.1.
    YP_004576474.1. NC_015636.1.

    Genome annotation databases

    EnsemblBacteriaiAEH06696; AEH06696; Metok_0719.
    GeneIDi10772860.
    KEGGimok:Metok_0719.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002792 Genomic DNA. Translation: AEH06696.1 .
    RefSeqi WP_013866882.1. NC_015636.1.
    YP_004576474.1. NC_015636.1.

    3D structure databases

    ProteinModelPortali F8AM17.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEH06696 ; AEH06696 ; Metok_0719 .
    GeneIDi 10772860.
    KEGGi mok:Metok_0719.

    Phylogenomic databases

    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci MOKI647113:GHB9-744-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Methanothermococcus okinawensis IH1."
      Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Held B., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Sieprawska-Lupa M., Takai K.
      , Miyazaki J., Whitman W., Woyke T.
      Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14208 / JCM 11175 / IH1Imported.

    Entry informationi

    Entry nameiF8AM17_METOI
    AccessioniPrimary (citable) accession number: F8AM17
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3