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F8AH01 (F8AH01_PYRYC) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase RuleBase RU000617 HAMAP-Rule MF_00407
EC=6.5.1.1 RuleBase RU000617 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:PYCH_03680 EMBL AEH24059.1
OrganismPyrococcus yayanosii (strain CH1 / JCM 16557) [Complete proteome] [HAMAP]
Taxonomic identifier529709 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus ›

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. RuleBase RU004196 HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2761N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2741ATP By similarity HAMAP-Rule MF_00407
Binding site2811ATP By similarity HAMAP-Rule MF_00407
Binding site2961ATP By similarity HAMAP-Rule MF_00407
Binding site3261ATP By similarity HAMAP-Rule MF_00407
Binding site3661ATP By similarity HAMAP-Rule MF_00407
Binding site4411ATP By similarity HAMAP-Rule MF_00407
Binding site4471ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
F8AH01 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 97387297BC0BFCE9

FASTA58866,835
        10         20         30         40         50         60 
MEGDGWRDIK VVRNPYFSLR IVPTVVPMRY LELAQLYQRL EKTTLKTLKT RLVADFLKKV 

        70         80         90        100        110        120 
PNDLLEIIPY LILGDVFPEW DERELGVGEK LLIRAVSMAT GVDAKEIENS VKETGDLGES 

       130        140        150        160        170        180 
VALAIKKRKQ RSFFSQPLTI KRVYDTLVKV AEISGEGSQD KKLKYLANLF MDAEPIEAKY 

       190        200        210        220        230        240 
LARTILGIMR TGVAEGILRD AIAEAFHVKV ELVERAYMLT SDFGFVTKVA KLEGNEGLAK 

       250        260        270        280        290        300 
VTIQVGKPIK PMLAQQAASI KDALLEMGGE AEFEIKYDGA RVQVHKDGDR IIVYSRRLEN 

       310        320        330        340        350        360 
VTRSIPEVVE RLKRAIKAEK AIVEGELVAV GEDGRPLPFQ YVLRRFRRKY NIEEMIEKIP 

       370        380        390        400        410        420 
LELNLFDVLY VGGKALIDEP FIERRKTLEG IIEADGWIKV AENLITKSVE EAESFYRKAL 

       430        440        450        460        470        480 
GMGHEGLMAK RLDSVYEPGN RGKKWLKIKP TMENLDLVII GAEWGEGRRA HVFGSFILGA 

       490        500        510        520        530        540 
YDPHTGEFVE VGKVGSGFTD EDLIEFTKML KRLITKEEGK RVWFEPKVVI EVTYQEIQKS 

       550        560        570        580 
PKYRSGFALR FPRYVALRDD KGPEDADTIE RIAQLYELQE RMKGKKIA

« Hide

References

[1]"Complete genome sequence of the obligate piezophilic hyperthermophilic archaeon Pyrococcus yayanosii CH1."
Jun X., Lupeng L., Minjuan X., Oger P., Fengping W., Jebbar M., Xiang X.
J. Bacteriol. 193:4297-4298(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CH1 / JCM 16557.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002779 Genomic DNA. Translation: AEH24059.1.
RefSeqYP_004623331.1. NC_015680.1.

3D structure databases

ProteinModelPortalF8AH01.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEH24059; AEH24059; PYCH_03680.
GeneID10836944.
KEGGpya:PYCH_03680.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK10747.
OMAARVQVHK.

Enzyme and pathway databases

BioCycPYAY529709:GHZ0-361-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF8AH01_PYRYC
AccessionPrimary (citable) accession number: F8AH01
Entry history
Integrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info