F8A9R3 (F8A9R3_THEID) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Acetolactate synthase RuleBase RU003591
EC=2.2.1.6 RuleBase RU003591

Gene names

Ordered Locus Names:

Thein_1918 EMBL AEH45773.1

Organism

Thermodesulfatator indicus (strain DSM 15286 / JCM 11887 / CIR29812) [Complete proteome] [HAMAP] EMBL AEH45773.1

Taxonomic identifier

667014 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermodesulfobacteria › Thermodesulfobacteria › Thermodesulfobacteriales › Thermodesulfobacteriaceae › Thermodesulfatator › Thermodesulfatator indicus

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Sequence similarities	Belongs to the TPP enzyme family. RuleBase RU003702

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis RuleBase RU003591
Ligand	Magnesium RuleBase RU003591 Metal-binding RuleBase RU003591 Thiamine pyrophosphate RuleBase RU003591
Molecular function	Transferase RuleBase RU003591
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	F8A9R3 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 1572D6BB4F426A21	FASTA	567	62,986
	10 20 30 40 50 60 MAKKMTGAQI VVEALRREGV DIIFGYPGGA VIDIYDELYR TEDIKHVLVR HEQGATHAAD 70 80 90 100 110 120 GLARSTGKVG VCLVTSGPGA TNTVTGIATA YMDSIPMVIL TGQVPTKLIG NDAFQEVDIT 130 140 150 160 170 180 GITRPCTKHN FLVKRVEDLA WTLKAAFHIA RTGRPGPVLV DLPKDVQQAK TEFHWPDEIR 190 200 210 220 230 240 LRSYNPNYEP NKRQVEKTYR LLEASTRPVF LIGGGVIASG AHEEVRELAE LLHIPVTMTL 250 260 270 280 290 300 MGLGGFPGTH EYSLGMLGMH GTYYANMAVA NSDLIIAVGA RFDDRVTGKV DAFAPMAKIV 310 320 330 340 350 360 HIDIDPTSIQ KNVRVDVPIV GDCKRSLAKL LEVVKSVARP SKLWKEQFKE WWEQIDIWRR 370 380 390 400 410 420 RYPLSYKQEG DYIKPQFVIE KMYELTKGDA IVSTEVGQNQ MWTAQFWKFD RPRTLLSSGG 430 440 450 460 470 480 LGTMGYGFPA AIGAQMAHPD KLVIDFAGDG SIQMNIQEMA TAIDQRLPIK VIILNNGFLG 490 500 510 520 530 540 MVRQWQELFY DRRYSSVEFA TIPDFVKLAE AYGAVGLRAT KPEEVEPVLK KALETKNLVF 550 560 VDIHIAPEEG VFPMVPAGRA TTEMILV

References

[1]

"The complete genome of Thermodesulfatator indicus DSM 15286." Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Peters L., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Saunders L., Detter J.C., Tapia R., Han C., Land M., Hauser L., Markowitz V. , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Schroeder M., Brambilla E., Klenk H.-P., Eisen J.A. Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15286 / JCM 11887 / CIR29812.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002683 Genomic DNA. Translation: AEH45773.1.
RefSeq	YP_004626737.1. NC_015681.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEH45773; AEH45773; Thein_1918.
GeneID	10842196.
KEGG	tid:Thein_1918.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01652.
Enzyme and pathway databases
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F8A9R3_THEID

Accession

Primary (citable) accession number: F8A9R3

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)