F6ZFR0 (MTD2L_CALJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2

Alternative name(s):
NADP-dependent methylenetetrahydrofolate dehydrogenase 2-like protein
Short name=MTHFD2-like

Including the following 2 domains:

1. NAD-dependent methylenetetrahydrofolate dehydrogenase
   EC=1.5.1.15
2. Methenyltetrahydrofolate cyclohydrolase
   EC=3.5.4.9

Gene names

Name:

MTHFD2L

Organism

Callithrix jacchus (White-tufted-ear marmoset) [Reference proteome]

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
Cofactor	Magnesium By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Magnesium NAD
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2		PRO_0000413327

Sequences

	Sequence		Length	Mass (Da)
	F6ZFR0 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: 0A425A0D6AF2797C	FASTA	347	37,342
	10 20 30 40 50 60 MTVPARGFLL LRGRLGRVPA LGRSTAPPVR APGGPRSAFR GFRSSGVRHE AVIISGTEMA 70 80 90 100 110 120 KHIQKEIKQG VESWISLGNR RPHLSIILVG DNPASHTYVR NKIRAASAVG ICSELILKPK 130 140 150 160 170 180 DVSQEELLDI TDQLNMDPRV SGILVQLPLP DHVDERMICN GIAPEKDVDG FHIINIGRLC 190 200 210 220 230 240 LDQHSLIPAT ASAVWEIITR TGIQTFGKNV VVAGRSKNVG MPIAMLLHTD GEHERPGGDA 250 260 270 280 290 300 TVTIAHRYTP KEQLKTHTQL ADVIIVAAGI PKLITSDMVK EGAAVIDVGI NYVHDPVTGK 310 320 330 340 TKLVGDVDFE AVKKKAGFIT PVPGGVGPMT VAMLLKNTLL AAKKIIY

References

[1]	Warren W., Ye L., Minx P., Worley K., Gibbs R., Wilson R.K. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACFV01093585 Genomic DNA. No translation available. ACFV01093586 Genomic DNA. No translation available. ACFV01093587 Genomic DNA. No translation available. ACFV01093588 Genomic DNA. No translation available.
RefSeq	XP_002745776.1. XM_002745730.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCJAT00000026964; ENSCJAP00000025515; ENSCJAG00000013883.
GeneID	100389511.
Organism-specific databases
CTD	441024.
Phylogenomic databases
GeneTree	ENSGT00630000089817.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProtoNet	Search...

Entry information

Entry name

MTD2L_CALJA

Accession

Primary (citable) accession number: F6ZFR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program