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F6Z5C0

- UBP15_XENTR

UniProt

F6Z5C0 - UBP15_XENTR

Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

usp15

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 2 (28 Nov 2012)
      Previous versions | rss
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    Functioni

    Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs, thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of tgfbr1, leading to an enhanced TGF-beta signal By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei297 – 2971NucleophilePROSITE-ProRule annotation
    Active sitei891 – 8911Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. BMP signaling pathway Source: UniProtKB
    2. monoubiquitinated protein deubiquitination Source: UniProtKB
    3. pathway-restricted SMAD protein phosphorylation Source: UniProtKB
    4. protein deubiquitination Source: UniProtKB
    5. transforming growth factor beta receptor signaling pathway Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_204688. Downregulation of TGF-beta receptor signaling.

    Protein family/group databases

    MEROPSiC19.022.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 15
    Ubiquitin thioesterase 15
    Ubiquitin-specific-processing protease 15
    Gene namesi
    Name:usp15
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-1008519. usp15.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 982982Ubiquitin carboxyl-terminal hydrolase 15PRO_0000420491Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000046428.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 118112DUSPPROSITE-ProRule annotationAdd
    BLAST
    Domaini288 – 933646USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 DUSP domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    KOiK11835.

    Family and domain databases

    Gene3Di3.30.2230.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR029071. Ubiquitin-rel_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: F6Z5C0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEGGVVDLE TQRSEVSALL KTPLRRGDTW YLIDSRWFKQ WKKYVGFDSW    50
    DKYQMGDQNV YPGPIDNSGL LKDTDTQSLK EHLIDELDYI LLPTEGWNKL 100
    VSWYSVVESQ QPIARKVVEQ GMFVKHCKVE VYLTELKLCE NGNMNNVVTR 150
    RFSKADTIDM IEKEMRTIFS IPDEKETRLW NKYMSNTFEP LNKPESTIQD 200
    AGLYQGQMLV IEQKNQEGQW PRGSMPKSSG IPNFSTLPKI SPSSLSNNYN 250
    NFNSRIMKNS NYCLPSYAAY KNYEYSEPGR HNEQPGLCGL SNLGNTCFMN 300
    SAIQCLSNTP PLTEYFLNDK YQDELNMDNP LGMRGEIAKS YAELIKQMWS 350
    GKYSYVTPRA FKTQVGRFAP QFSGYQQQDC QELLAFLLDG LHEDLNRIRK 400
    KPYIQLKDAD GRPDKVVAEE AWENHIKRND SIIVDIFHGL FKSTLVCPEC 450
    SKISVTFDPF CYLTLPLPMK KERALEVYLV RMDPLAKPMQ YKVIVPKIGN 500
    IMDLCTALSS LSGIAPEKMV VTDIYNHRFH RIFAMDENLS SIMERDDIYV 550
    FETSINRTED TEQVIIPVYL REKFRHTSYS HHHGSTLFGQ PFLITVPRNI 600
    TEDKLYNLLL LRMCRYVKAT NDTEENDGSL HCNKEHTVNG NGPNGIHEEG 650
    SPSEMETDEP DDESSQDQEL PSENENSQSE DSVGGDNDSE NGLCTEDTCK 700
    GQSVTGQKKR LFTFQFSNLG SSDITYIKDD TKYIRFDERQ LRLDERSYLA 750
    LDWDPKLKKK FFDENAAEDF EKHESVDFTP QKKAFMKLKD CIELFTTKEK 800
    LGAEDPWYCP NCKEHQQATK KLDLWSLPPV LVVHLKRFSY SRYMRDKLDT 850
    LVDFPISDLD MSTFLINPNA GPCCYNLIAV SNHYGGMGGG HYTAFAKNKD 900
    DGKWYYFDDS SVSTASEEQI VSKAAYVLFY QRQDTITGTG FFPLDKEVKQ 950
    GASAATGAPH ESDEESNEDE NDIENENCMH TN 982
    Length:982
    Mass (Da):112,689
    Last modified:November 28, 2012 - v2
    Checksum:i2B4F415FE1199C24
    GO
    Isoform 2 (identifier: F6Z5C0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         227-256: KSSGIPNFSTLPKISPSSLSNNYNNFNSRI → N

    Show »
    Length:953
    Mass (Da):109,536
    Checksum:iE87676D21A7742C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti626 – 6261N → I in AAI66318. 1 PublicationCurated
    Sequence conflicti978 – 9825CMHTN → YLEKL in AAI66318. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei227 – 25630KSSGI…FNSRI → N in isoform 2. 1 PublicationVSP_044519Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAMC01103598 Genomic DNA. No translation available.
    AAMC01103599 Genomic DNA. No translation available.
    AAMC01103600 Genomic DNA. No translation available.
    AAMC01103601 Genomic DNA. No translation available.
    BC166318 mRNA. Translation: AAI66318.1.
    RefSeqiNP_001121498.1. NM_001128026.1.
    UniGeneiStr.21191.

    Genome annotation databases

    GeneIDi100158604.
    KEGGixtr:100158604.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAMC01103598 Genomic DNA. No translation available.
    AAMC01103599 Genomic DNA. No translation available.
    AAMC01103600 Genomic DNA. No translation available.
    AAMC01103601 Genomic DNA. No translation available.
    BC166318 mRNA. Translation: AAI66318.1 .
    RefSeqi NP_001121498.1. NM_001128026.1.
    UniGenei Str.21191.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000046428.

    Protein family/group databases

    MEROPSi C19.022.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100158604.
    KEGGi xtr:100158604.

    Organism-specific databases

    CTDi 9958.
    Xenbasei XB-GENE-1008519. usp15.

    Phylogenomic databases

    KOi K11835.

    Enzyme and pathway databases

    Reactomei REACT_204688. Downregulation of TGF-beta receptor signaling.

    Family and domain databases

    Gene3Di 3.30.2230.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR029071. Ubiquitin-rel_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryo.

    Entry informationi

    Entry nameiUBP15_XENTR
    AccessioniPrimary (citable) accession number: F6Z5C0
    Secondary accession number(s): B2GUK6, F6ZNA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: November 28, 2012
    Last modified: October 1, 2014
    This is version 22 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3