ID   DMRT3_HORSE             Reviewed;         474 AA.
AC   F6W2R2; J3SGP7;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 2.
DT   24-JAN-2024, entry version 58.
DE   RecName: Full=Doublesex and mab-3 related transcription factor 3;
GN   Name=DMRT3;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   POLYMORPHISM.
RC   STRAIN=Thoroughbred;
RX   PubMed=22932389; DOI=10.1038/nature11399;
RA   Andersson L.S., Larhammar M., Memic F., Wootz H., Schwochow D.,
RA   Rubin C.-J., Patra K., Arnason T., Wellbring L., Hjalm G., Imsland F.,
RA   Petersen J.L., McCue M.E., Mickelson J.R., Cothran G., Ahituv N.,
RA   Roepstorff L., Mikko S., Vallstedt A., Lindgren G., Andersson L.,
RA   Kullander K.;
RT   "Mutations in DMRT3 affect locomotion in horses and spinal circuit function
RT   in mice.";
RL   Nature 488:642-646(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Thoroughbred;
RX   PubMed=19892987; DOI=10.1126/science.1178158;
RA   Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA   Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA   Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA   Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA   Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA   Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA   Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA   Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA   Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA   Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT   "Genome sequence, comparative analysis, and population genetics of the
RT   domestic horse.";
RL   Science 326:865-867(2009).
CC   -!- FUNCTION: Probable transcription factor that plays a role in
CC       configuring the spinal circuits controlling stride in vertebrates.
CC       Involved in neuronal specification within a specific subdivision of
CC       spinal cord neurons and in the development of a coordinated locomotor
CC       network controlling limb movements. May regulate transcription during
CC       sexual development. {ECO:0000269|PubMed:22932389}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
CC   -!- TISSUE SPECIFICITY: Expressed in the spinal cord, in a small population
CC       of neurons located in the ventral horn and around the central canal.
CC       {ECO:0000269|PubMed:22932389}.
CC   -!- DOMAIN: DMA domain interacts with ubiquitin.
CC       {ECO:0000250|UniProtKB:Q9NQL9}.
CC   -!- POLYMORPHISM: This sequence corresponds to the wild-type form. A
CC       truncated allele, allele A (AC J7FCF0), is linked to the ability to
CC       perform alternate gaits, which can be either pace or four-beat ambling
CC       gaits. The allele A sequence (AC J7FCF0) has a favorable effect on
CC       harness racing performance and on the diversification of the domestic
CC       horse, as the altered gait characteristics of a number of breeds
CC       apparently require this mutation. Homozygosity for the mutation is
CC       required, but not sufficient for pacing, as many Standardbred trotters
CC       and some Icelandic horses that are homozygous for this mutation do not
CC       pace. The allele A is not found in the following horse populations:
CC       Arabian horse, Gotland pony, North-Swedish draft horse, Przewalski's
CC       horse, Shetland pony, Swedish ardennes, Swedish warmblood and
CC       Thoroughbred.
CC   -!- MISCELLANEOUS: DMRT3 is a marker for a subset of spinal cord neurons
CC       (dI6).
CC   -!- SIMILARITY: Belongs to the DMRT family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=A gait on the wildside
CC       - Issue 154 of November 2013;
CC       URL="https://web.expasy.org/spotlight/back_issues/154/";
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DR   EMBL; JQ922365; AFK83802.1; -; Genomic_DNA.
DR   EMBL; JQ922367; AFK83804.1; -; Genomic_DNA.
DR   EMBL; JQ922371; AFK83806.1; -; mRNA.
DR   RefSeq; NP_001304194.1; NM_001317265.1.
DR   AlphaFoldDB; F6W2R2; -.
DR   SMR; F6W2R2; -.
DR   Ensembl; ENSECAT00000025062.2; ENSECAP00000020841.2; ENSECAG00000023412.4.
DR   GeneID; 100147177; -.
DR   KEGG; ecb:100147177; -.
DR   CTD; 58524; -.
DR   GeneTree; ENSGT00940000160420; -.
DR   HOGENOM; CLU_052449_0_0_1; -.
DR   InParanoid; F6W2R2; -.
DR   OrthoDB; 2915919at2759; -.
DR   Proteomes; UP000002281; Chromosome 23.
DR   Bgee; ENSECAG00000023412; Expressed in testis and 2 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IMP:AgBase.
DR   GO; GO:0003677; F:DNA binding; IMP:AgBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0008344; P:adult locomotory behavior; IMP:AgBase.
DR   GO; GO:0007628; P:adult walking behavior; IEA:Ensembl.
DR   GO; GO:0046661; P:male sex differentiation; IBA:GO_Central.
DR   GO; GO:0042487; P:regulation of odontogenesis of dentin-containing tooth; IEA:Ensembl.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0019226; P:transmission of nerve impulse; ISS:AgBase.
DR   GO; GO:0021521; P:ventral spinal cord interneuron specification; ISS:AgBase.
DR   Gene3D; 4.10.1040.10; DM DNA-binding domain; 1.
DR   InterPro; IPR001275; DM_DNA-bd.
DR   InterPro; IPR036407; DM_DNA-bd_sf.
DR   InterPro; IPR005173; DMA.
DR   InterPro; IPR026607; DMRT.
DR   InterPro; IPR009060; UBA-like_sf.
DR   PANTHER; PTHR12322; DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT; 1.
DR   PANTHER; PTHR12322:SF120; DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 3; 1.
DR   Pfam; PF00751; DM; 1.
DR   Pfam; PF03474; DMA; 1.
DR   SMART; SM00301; DM; 1.
DR   SUPFAM; SSF82927; Cysteine-rich DNA binding domain, (DM domain); 1.
DR   SUPFAM; SSF46934; UBA-like; 1.
DR   PROSITE; PS40000; DM_1; 1.
DR   PROSITE; PS50809; DM_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW   Nucleus; Reference proteome; Sexual differentiation; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..474
FT                   /note="Doublesex and mab-3 related transcription factor 3"
FT                   /id="PRO_0000422701"
FT   DOMAIN          251..286
FT                   /note="DMA"
FT                   /evidence="ECO:0000255"
FT   DNA_BIND        29..76
FT                   /note="DM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT   REGION          89..130
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          157..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          415..474
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..126
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        157..183
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   474 AA;  51418 MW;  C721D1DDACC433C0 CRC64;
     MNGYGSPYLY MGGPVSQPPR APLQRTPKCA RCRNHGVLSW LKGHKRYCRF KDCTCEKCIL
     IIERQRVMAA QVALRRQQAN ESLESLIPDS LRALPGPPPP GDAAAAAPQP PPTSQPSQPP
     PPQRPAAELA AAAALRWATE PQPGALQAQL AKPDLTEERL GDGSSADNTE TFSDKDTDQR
     SSPDVVKSKG CFTPESPEVV SVDEGGYAVQ KNGGTSESRP DSPKYHGEQN HLLIEGPSGT
     VSLPFSLKAN RPPLEVLKKI FPNQKPTVLE LILKGCGGDL VSAVEVLLSS RSSASAADRT
     SAEPESLVLP SNGHIFEHTL SSYPISSSKW SVGSAFRVPD TLRFSADSSN VVPNPLAVPL
     QHPFPQPPRY PLMLRNTLAR NQSSPFLPND VTLWNTMTLQ QQYQLRSQYV SPFPGSSPSV
     FRSSPVLPTR APEDPRISIP DDGCPIVSKQ SLYTEDDYDE RSDSSDSRIL NTSS
//