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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtap

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathway:iL-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtap)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151PhosphateUniRule annotation
Sitei176 – 1761Important for substrate specificityUniRule annotation
Binding sitei194 – 1941Substrate; via amide nitrogenUniRule annotation
Binding sitei195 – 1951PhosphateUniRule annotation
Sitei231 – 2311Important for substrate specificityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

ReactomeiREACT_301908. Methionine salvage pathway.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
Name:mtap
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281S-methyl-5'-thioadenosine phosphorylasePRO_0000415115Add
BLAST

Expressioni

Gene expression databases

BgeeiF6V515.
ExpressionAtlasiF6V515. baseline.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi8364.ENSXETP00000012027.

Structurei

3D structure databases

ProteinModelPortaliF6V515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 592Phosphate bindingUniRule annotation
Regioni91 – 922Phosphate bindingUniRule annotation
Regioni218 – 2203Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074874.
InParanoidiF6V515.
OMAiMTNHTEA.
OrthoDBiEOG771270.
TreeFamiTF312883.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

F6V515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVCAVKVG IIGGSGLDDP DILEGRLEKY VDTPFGKPSD ALVLGKIKNV
60 70 80 90 100
DCVLLASRHG RQHTIAPTNV NYRANIWALK SEGCTHILVT TACGSLREEI
110 120 130 140 150
QPGDIVIVDQ FIDRTTKREQ TFYDGGPSCL PGVCHIPMAE PFCAKTREVL
160 170 180 190 200
IDIAKRLGIK CHSKGAMITI EGPRFSSKAE SQMFRLWGAD VINMTTVPEV
210 220 230 240 250
ILAKEAGICY ASIAMATDYD CWKEHEEAVS VDRVLKTLKE NANKATSILL
260 270 280
TAIPQIAAMD WTELLQSMKS TVQLSVMLPK H
Length:281
Mass (Da):30,699
Last modified:July 27, 2011 - v1
Checksum:i096C64113C943764
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01028797 Genomic DNA. No translation available.
AAMC01028798 Genomic DNA. No translation available.

Genome annotation databases

EnsembliENSXETT00000012027; ENSXETP00000012027; ENSXETG00000005467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAMC01028797 Genomic DNA. No translation available.
AAMC01028798 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliF6V515.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000012027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSXETT00000012027; ENSXETP00000012027; ENSXETG00000005467.

Phylogenomic databases

GeneTreeiENSGT00550000074874.
InParanoidiF6V515.
OMAiMTNHTEA.
OrthoDBiEOG771270.
TreeFamiTF312883.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.
ReactomeiREACT_301908. Methionine salvage pathway.

Gene expression databases

BgeeiF6V515.
ExpressionAtlasiF6V515. baseline.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiMTAP_XENTR
AccessioniPrimary (citable) accession number: F6V515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.