ID   F6UUY1_XENTR            Unreviewed;       588 AA.
AC   F6UUY1;
DT   27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT   02-JUN-2021, sequence version 4.
DT   27-MAR-2024, entry version 75.
DE   RecName: Full=Glutamate decarboxylase 2 {ECO:0000256|ARBA:ARBA00040577};
DE            EC=4.1.1.15 {ECO:0000256|ARBA:ARBA00012421};
DE   AltName: Full=65 kDa glutamic acid decarboxylase {ECO:0000256|ARBA:ARBA00043203};
DE   AltName: Full=Glutamate decarboxylase 65 kDa isoform {ECO:0000256|ARBA:ARBA00041381};
GN   Name=gad2 {ECO:0000313|Ensembl:ENSXETP00000040531,
GN   ECO:0000313|RefSeq:XP_017950266.1,
GN   ECO:0000313|Xenbase:XB-GENE-976598};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364 {ECO:0000313|Ensembl:ENSXETP00000040531};
RN   [1] {ECO:0000313|Ensembl:ENSXETP00000040531}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nigerian {ECO:0000313|Ensembl:ENSXETP00000040531};
RX   PubMed=20431018; DOI=10.1126/science.1183670;
RA   Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA   Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA   Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA   Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA   Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA   Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA   Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA   Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA   Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT   "The genome of the Western clawed frog Xenopus tropicalis.";
RL   Science 328:633-636(2010).
RN   [2] {ECO:0000313|Ensembl:ENSXETP00000040531}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (JUN-2011) to UniProtKB.
RN   [3] {ECO:0000313|RefSeq:XP_017950266.1}
RP   IDENTIFICATION.
RC   STRAIN=Nigerian {ECO:0000313|RefSeq:XP_017950266.1};
RC   TISSUE=Liver and blood {ECO:0000313|RefSeq:XP_017950266.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Catalyzes the production of GABA.
CC       {ECO:0000256|ARBA:ARBA00037048}.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|PIRSR:PIRSR602129-50, ECO:0000256|RuleBase:RU000382};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004193};
CC       Lipid-anchor {ECO:0000256|ARBA:ARBA00004193}. Cytoplasm, cytosol
CC       {ECO:0000256|ARBA:ARBA00004514}. Cytoplasmic vesicle
CC       {ECO:0000256|ARBA:ARBA00004541}. Golgi apparatus membrane
CC       {ECO:0000256|ARBA:ARBA00004255}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004255}; Cytoplasmic side
CC       {ECO:0000256|ARBA:ARBA00004255}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004635}; Lipid-anchor
CC       {ECO:0000256|ARBA:ARBA00004635}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC       {ECO:0000256|ARBA:ARBA00004287}. Presynaptic cell membrane
CC       {ECO:0000256|ARBA:ARBA00037840}; Lipid-anchor
CC       {ECO:0000256|ARBA:ARBA00037840}. Vesicle
CC       {ECO:0000256|ARBA:ARBA00004373}.
CC   -!- SIMILARITY: Belongs to the group II decarboxylase family.
CC       {ECO:0000256|ARBA:ARBA00009533, ECO:0000256|RuleBase:RU000382}.
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DR   RefSeq; XP_017950266.1; XM_018094777.2.
DR   Ensembl; ENSXETT00000040531; ENSXETP00000040531; ENSXETG00000026517.
DR   GeneID; 100493806; -.
DR   KEGG; xtr:100493806; -.
DR   AGR; Xenbase:XB-GENE-976598; -.
DR   CTD; 2572; -.
DR   Xenbase; XB-GENE-976598; gad2.
DR   eggNOG; KOG0629; Eukaryota.
DR   HOGENOM; CLU_011856_0_0_1; -.
DR   OMA; AGMVIFK; -.
DR   PhylomeDB; F6UUY1; -.
DR   TreeFam; TF314688; -.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Bgee; ENSXETG00000026517; Expressed in brain and 1 other cell type or tissue.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042734; C:presynaptic membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016831; F:carboxy-lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0009449; P:gamma-aminobutyric acid biosynthetic process; IEA:Ensembl.
DR   CDD; cd06450; DOPA_deC_like; 1.
DR   Gene3D; 3.90.1150.170; -; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR002129; PyrdxlP-dep_de-COase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR021115; Pyridoxal-P_BS.
DR   PANTHER; PTHR45677:SF11; GLUTAMATE DECARBOXYLASE 2; 1.
DR   PANTHER; PTHR45677; GLUTAMATE DECARBOXYLASE-RELATED; 1.
DR   Pfam; PF00282; Pyridoxal_deC; 1.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR   PROSITE; PS00392; DDC_GAD_HDC_YDC; 1.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW   Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329};
KW   Decarboxylase {ECO:0000256|ARBA:ARBA00022793};
KW   Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW   Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU000382};
KW   Membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Palmitate {ECO:0000256|ARBA:ARBA00023139};
KW   Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR602129-50,
KW   ECO:0000256|RuleBase:RU000382};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008143};
KW   Synapse {ECO:0000256|ARBA:ARBA00023018}.
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         399
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR602129-50"
SQ   SEQUENCE   588 AA;  65720 MW;  14B23FFA01451AAF CRC64;
     MASPGSGFWP FGAEEGNKEA ENPGTAKAWC QVAQKFTGGI SSKLCSLLYG EGERAPGSVG
     KNEKDELGSG ERGMACNCDT KACGCRKGEA NFSLLYSTDL LPSKNGEGVT MTFLQDVIDV
     LLQYVVQTFD RSTKVIDFHY PNELLQAYNW ELSEQPQSLD GILLNCKTTL KYAIKTGHPR
     YFNQLSTGLD MVGLAADWLT STANTNMFTY EIAPVFVLLE YVTLKKMREM IGWPGGSGDG
     IFSPGGAISN LYAMLIARFK MFPEVKEKGM AALPRLVAFT SEHSHFSVKK GAAALGIGTD
     SVILIKADER GRLIPSDLER RIIEAKQNGY VPFFVSATAG TTVYGAFDPL VSIADICKKY
     RIWMHVDAAW GGGLLMSRKH KWKLNGADRA NSVTWNPHKM MGVPLQCSAL LVREEGLMQN
     CNQMHASYLF QQDKHYDLSY DTGDKALQCG RHVDIFKLWL MWRAKGTTGF EAHIDKCLEL
     SEYLYNKIKN REGYEMVIEG KPQHTNVCFW YVPPSLRNME DNEERMNRLS KVAPIIKARM
     MEYGTTMVSY QPHGDKGNFF RMVISNPAAA HQDIDFLIEE IERLGQDL
//