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F6S675 (IMDH1_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inosine-5'-monophosphate dehydrogenase 1

Short name=IMP dehydrogenase 1
Short name=IMPD 1
Short name=IMPDH 1
EC=1.1.1.205
Gene names
Name:impdh1
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity. HAMAP-Rule MF_03156

Catalytic activity

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH. HAMAP-Rule MF_03156

Cofactor

Potassium By similarity. HAMAP-Rule MF_03156

Enzyme regulation

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity. HAMAP-Rule MF_03156

Pathway

Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. HAMAP-Rule MF_03156

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03156

Subcellular location

Cytoplasm By similarity. Nucleus By similarity HAMAP-Rule MF_03156.

Sequence similarities

Belongs to the IMPDH/GMPR family.

Contains 2 CBS domains.

Ontologies

Keywords
   Biological processGMP biosynthesis
Purine biosynthesis
   Cellular componentCytoplasm
Nucleus
   DomainCBS domain
Repeat
   LigandMetal-binding
NAD
Potassium
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionIMP dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

adenyl nucleotide binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Inosine-5'-monophosphate dehydrogenase 1 HAMAP-Rule MF_03156
PRO_0000415678

Regions

Domain112 – 17160CBS 1
Domain177 – 23559CBS 2
Nucleotide binding272 – 2743NAD By similarity
Nucleotide binding322 – 3243NAD By similarity
Region362 – 3643IMP binding By similarity
Region385 – 3862IMP binding By similarity
Region409 – 4135IMP binding By similarity

Sites

Active site3291Thioimidate intermediate By similarity
Metal binding3241Potassium; via carbonyl oxygen By similarity
Metal binding3261Potassium; via carbonyl oxygen By similarity
Metal binding3291Potassium; via carbonyl oxygen By similarity
Metal binding4981Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal binding4991Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal binding5001Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Binding site3271IMP By similarity
Binding site4391IMP By similarity

Sequences

Sequence LengthMass (Da)Tools
F6S675 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 6FAEE3FAE9E12ED0

FASTA51255,454
        10         20         30         40         50         60 
MADYLISGGT GYVPEDGLTA QHLFANSDGL TYNDFLILPG FIDFTADEVD LTSALTRKIT 

        70         80         90        100        110        120 
LKTPLISSPM DTVTESDMAI AMALMGGIGI IHHNCTPEFQ ANEVRKKFEQ GFITDPVVMS 

       130        140        150        160        170        180 
LNHTVGDVFE AKNRHGFSGI PVTETGKMGS KLVGIVTSRD IDFLTEKDYS TYLSEVMTKR 

       190        200        210        220        230        240 
DELVVAPAGV TLKEANEILQ RSKKGKLPIV NDSDELVAII ARTDLKKNRD YPLASKDCRK 

       250        260        270        280        290        300 
QLLCGAAIGT REDDKYRLDL LTQAGVDVVV LDSSQGNSVY QINMIHYIKQ KYPELQVVGG 

       310        320        330        340        350        360 
NVVTAAQAKN LIDAGVDALR VGMGCGSICI TQEVMACGRP QGTAVYKVAE YARRFGVPVI 

       370        380        390        400        410        420 
ADGGIQTVGH VVKALALGAS TVMMGSLLAA TTEAPGEYFF SDGVRLKKYR GMGSLDAMEK 

       430        440        450        460        470        480 
NTSSQKRYFS EGDKVKVAQG VSGSIQDKGS IHKFVPYLIA GIQHGCQDIG AKSLSILRSM 

       490        500        510 
MYSGELKLEK RTMSAQVEGG VHGLHSYEKR LY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAMC01092195 Genomic DNA. No translation available.
AAMC01092196 Genomic DNA. No translation available.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000065632; ENSXETP00000060849; ENSXETG00000023047.

Phylogenomic databases

GeneTreeENSGT00530000062923.
OrthoDBEOG7VTDMM.

Enzyme and pathway databases

UniPathwayUPA00601; UER00295.

Gene expression databases

BgeeF6S675.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01964. IMPDH.
InterProIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFPIRSF000130. IMPDH. 1 hit.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsTIGR01302. IMP_dehydrog. 1 hit.
PROSITEPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIMDH1_XENTR
AccessionPrimary (citable) accession number: F6S675
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways