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Protein

Malate synthase G

Gene

glcB

Organism
Novosphingobium sp. PP1Y
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei254 – 2541Acetyl-CoAUniRule annotation
Binding sitei291 – 2911Acetyl-CoAUniRule annotation
Active sitei318 – 3181Proton acceptorUniRule annotation
Binding sitei318 – 3181GlyoxylateUniRule annotation
Metal bindingi410 – 4101MagnesiumUniRule annotation
Binding sitei410 – 4101GlyoxylateUniRule annotation
Metal bindingi438 – 4381MagnesiumUniRule annotation
Binding sitei519 – 5191Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei609 – 6091Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

AcyltransferaseImported, Transferase

Keywords - Biological processi

Glyoxylate bypassUniRule annotationSAAS annotation, Tricarboxylic acid cycleUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciNSP702113:GJD2-1971-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
ORF Names:PP1Y_AT20390Imported
OrganismiNovosphingobium sp. PP1YImported
Taxonomic identifieri702113 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeNovosphingobium
Proteomesi
  • UP000009242 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei595 – 5951Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

OxidationUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi702113.PP1Y_AT20390.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni435 – 4384Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
KOiK01638.
OrthoDBiPOG091H05P0.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

F6INB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRVERSGL QIDAGLAAFV EEKVLAPIGQ DTDAFWSGFA QLLARFAPRN
60 70 80 90 100
RELLEKRDHL QSQIDAWHMS RAGRPIEQGE YQAFLQDIGY LVPEPEVFSI
110 120 130 140 150
GTRNVDPEIA TMAGPQLVVP VLNARFLLNA ANARWGSLYD AFYGTDALDA
160 170 180 190 200
APAQGKGYDP VRGAAVIAEG RKFLDKALPL ASGSWADVDS LEVTLVDPDQ
210 220 230 240 250
CVGETRKGCL FKSNGLHIEI VVDPSSQVGA TDKAGISDIV LEAALTTIVD
260 270 280 290 300
LEDSVAAVDA EDKLLAYSNW LGVIRGDLTD TFEKGGQTLT RELAGNKVVT
310 320 330 340 350
VPTGEAQELP GRSLLFVRNV GHLMTNPAIL LPDGSEIPEG IMDAVITSAI
360 370 380 390 400
SAQDVKGMTR YGNSRKGSIY IVKPKMHGPE ECSFTNDLFD AVEDLLQLPR
410 420 430 440 450
NTVKVGVMDE ERRTSANLAA CIHAVKDRIV FINTGFLDRT GDEIHTSMRA
460 470 480 490 500
GPMIRKSAIK GSTWIAAYEK RNVAIGLRHG LSGVAQIGKG MWAAPDMMRD
510 520 530 540 550
MMEQKVGHPR TGANTAWVPS PTAATLHAMH YHEVDVFGLR EGIAAEEIPG
560 570 580 590 600
LDLLLQIPLA DGANWSEDEV REELENNAQG LLGYVVRWID QGVGCSKVPD
610 620 630 640 650
INDVGLMEDR ATLRISSQHM ANWLLHGVCT EAQVMDALKR MAEKVDAQNA
660 670 680 690 700
GDPLYAPMAG NWDTSLAFKA ACDLVFKGVE QPSGYTEPLL HAWRLKKKSA

LVTA
Length:704
Mass (Da):76,484
Last modified:July 27, 2011 - v1
Checksum:iCDA840251055D982
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR856862 Genomic DNA. Translation: CCA92916.1.
RefSeqiWP_013833231.1. NC_015580.1.

Genome annotation databases

EnsemblBacteriaiCCA92916; CCA92916; PP1Y_AT20390.
KEGGinpp:PP1Y_AT20390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR856862 Genomic DNA. Translation: CCA92916.1.
RefSeqiWP_013833231.1. NC_015580.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi702113.PP1Y_AT20390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCA92916; CCA92916; PP1Y_AT20390.
KEGGinpp:PP1Y_AT20390.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
KOiK01638.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciNSP702113:GJD2-1971-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF6INB4_9SPHN
AccessioniPrimary (citable) accession number: F6INB4
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.