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Protein

Isocitrate dehydrogenase [NADP]

Gene

VIT_14s0066g00950

Organism
Vitis vinifera (Grape)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471SubstrateUniRule annotation
Binding sitei152 – 1521NADPUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Binding sitei202 – 2021SubstrateUniRule annotation
Sitei209 – 2091Critical for catalysisUniRule annotation
Sitei281 – 2811Critical for catalysisUniRule annotation
Metal bindingi321 – 3211Magnesium or manganeseUniRule annotation
Binding sitei329 – 3291NADPUniRule annotation
Metal bindingi344 – 3441Magnesium or manganeseUniRule annotation
Binding sitei397 – 3971NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi145 – 1473NADPUniRule annotation
Nucleotide bindingi379 – 3846NADPUniRule annotation

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: EnsemblPlants/Gramene
  3. NAD binding Source: EnsemblPlants/Gramene

GO - Biological processi

  1. isocitrate metabolic process Source: EnsemblPlants/Gramene
  2. response to stress Source: EnsemblPlants/Gramene
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:VIT_14s0066g00950Imported
OrganismiVitis vinifera (Grape)Imported
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis
ProteomesiUP000009183: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
  2. chloroplast thylakoid Source: EnsemblPlants/Gramene
  3. mitochondrion Source: EnsemblPlants/Gramene
Complete GO annotation...

Structurei

3D structure databases

ProteinModelPortaliF6HUY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1707Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

InParanoidiF6HUY0.
KOiK00031.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F6HUY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVLRPKLR FGAMSGAAIF PSSSSLALKN PNLSISSDQR FFNGILFKNG
60 70 80 90 100
SILHDRFPNA SLRCFASAAT FDRVRVQNPI VEMDGDEMAR IMWRMIKDKL
110 120 130 140 150
IFPYLDLDIR YFDLGILNRD ATDDKVTVES AEATLKYNVA VKCATITPDE
160 170 180 190 200
ARVKEFGLKS MWRSPNGTIR NILNGTVFRE PILCQNVPRI VPGWKKPICI
210 220 230 240 250
GRHAFGDQYR ATDTVVEGPG KLKLVFAPEN GDPPVELNVY DFKGPGIALA
260 270 280 290 300
MYNVDESIRA FAESSMSLAF AKKWPLYLST KNTILKKYDG RFKDIFQDVY
310 320 330 340 350
EENWKQKFEE HSIWYEHRLI DDMVAYALKS DGGYVWACKN YDGDVQSDLL
360 370 380 390 400
AQGFGSLGLM TSVLLSSDGK TLEAEAAHGT VTRHFRQYQK GLETSTNSIA
410 420 430 440 450
SIFAWTRGLE HRAKLDKNER LLDFVHKLEA ACIETVESGN MTKDLAILIH
460 470 480
GPKASKEFYL NTEEFIDTVA HNLEAKQFCN QIHSYS
Length:486
Mass (Da):54,938
Last modified:July 27, 2011 - v1
Checksum:i173600B93C85B3D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN596252 Genomic DNA. Translation: CCB58498.1.
RefSeqiXP_002276200.2. XM_002276164.3.

Genome annotation databases

EnsemblPlantsiVIT_14s0066g00950.t01; VIT_14s0066g00950.t01; VIT_14s0066g00950.
GeneIDi100249290.
KEGGivvi:100249290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN596252 Genomic DNA. Translation: CCB58498.1.
RefSeqiXP_002276200.2. XM_002276164.3.

3D structure databases

ProteinModelPortaliF6HUY0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiVIT_14s0066g00950.t01; VIT_14s0066g00950.t01; VIT_14s0066g00950.
GeneIDi100249290.
KEGGivvi:100249290.

Phylogenomic databases

InParanoidiF6HUY0.
KOiK00031.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla."
    The French-Italian Public Consortium for Grapevine Genome Characterization.
    Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.
    , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.
    Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir / PN40024Imported.

Entry informationi

Entry nameiF6HUY0_VITVI
AccessioniPrimary (citable) accession number: F6HUY0
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.