F6HUY0 (F6HUY0_VITVI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Ordered Locus Names:

VIT_14s0066g00950 EMBL CCB58498.1

Organism

Vitis vinifera (Grape) [Reference proteome]

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › rosids incertae sedis › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRSR PIRSR000108-3 PIRNR PIRNR000108
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRSR PIRSR000108-3 PIRNR PIRNR000108 Manganese PIRSR PIRSR000108-3 PIRNR PIRNR000108 Metal-binding PIRSR PIRSR000108-3 PIRNR PIRNR000108 NADP PIRSR PIRSR000108-4 PIRNR PIRNR000108
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	145 – 147	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	379 – 384	6	NADP By similarity PIRSR PIRSR000108-4
	Region	164 – 170	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	321	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	344	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	147	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	152	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	179	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	202	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	329	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	397	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	209	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	281	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	F6HUY0 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: 173600B93C85B3D3	FASTA	486	54,938
	10 20 30 40 50 60 MLRVLRPKLR FGAMSGAAIF PSSSSLALKN PNLSISSDQR FFNGILFKNG SILHDRFPNA 70 80 90 100 110 120 SLRCFASAAT FDRVRVQNPI VEMDGDEMAR IMWRMIKDKL IFPYLDLDIR YFDLGILNRD 130 140 150 160 170 180 ATDDKVTVES AEATLKYNVA VKCATITPDE ARVKEFGLKS MWRSPNGTIR NILNGTVFRE 190 200 210 220 230 240 PILCQNVPRI VPGWKKPICI GRHAFGDQYR ATDTVVEGPG KLKLVFAPEN GDPPVELNVY 250 260 270 280 290 300 DFKGPGIALA MYNVDESIRA FAESSMSLAF AKKWPLYLST KNTILKKYDG RFKDIFQDVY 310 320 330 340 350 360 EENWKQKFEE HSIWYEHRLI DDMVAYALKS DGGYVWACKN YDGDVQSDLL AQGFGSLGLM 370 380 390 400 410 420 TSVLLSSDGK TLEAEAAHGT VTRHFRQYQK GLETSTNSIA SIFAWTRGLE HRAKLDKNER 430 440 450 460 470 480 LLDFVHKLEA ACIETVESGN MTKDLAILIH GPKASKEFYL NTEEFIDTVA HNLEAKQFCN QIHSYS

References

[1]

"The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla." The French-Italian Public Consortium for Grapevine Genome Characterization. Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J. , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P. Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Pinot noir / PN40024.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN596252 Genomic DNA. Translation: CCB58498.1.
RefSeq	XP_002276200.2. XM_002276164.2.
UniGene	Vvi.1079.
3D structure databases
ProteinModelPortal	F6HUY0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	VIT_14s0066g00950.t01; VIT_14s0066g00950.t01; VIT_14s0066g00950.
GeneID	100249290.
KEGG	vvi:100249290.
Phylogenomic databases
KO	K00031.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F6HUY0_VITVI

Accession

Primary (citable) accession number: F6HUY0

Entry history

Integrated into UniProtKB/TrEMBL:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)