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Protein

Kynureninase

Gene

kynU

Organism
Ralstonia solanacearum (strain Po82)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation
L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • pyridoxal 5'-phosphateUniRule annotation
  • pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981Pyridoxal phosphate; via amide nitrogenUniRule annotation
Binding sitei99 – 991Pyridoxal phosphateUniRule annotation
Binding sitei173 – 1731Pyridoxal phosphateUniRule annotation
Binding sitei202 – 2021Pyridoxal phosphateUniRule annotation
Binding sitei205 – 2051Pyridoxal phosphateUniRule annotation
Binding sitei227 – 2271Pyridoxal phosphateUniRule annotation
Binding sitei257 – 2571Pyridoxal phosphateUniRule annotation
Binding sitei283 – 2831Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. kynureninase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-HAMAP
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. L-kynurenine catabolic process Source: UniProtKB-UniPathway
  4. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  5. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Pyridine nucleotide biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciRSOL1031711:GLJ5-2650-MONOMER.
UniPathwayiUPA00253; UER00329.

Names & Taxonomyi

Protein namesi
Recommended name:
KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
L-kynurenine hydrolaseUniRule annotation
Gene namesi
Name:kynUUniRule annotationImported
Ordered Locus Names:RSPO_c02606Imported
OrganismiRalstonia solanacearum (strain Po82)Imported
Taxonomic identifieri1031711 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000007953: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1334Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

KOiK01556.
OMAiRFWQPLS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

F6G4U4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLTRNTCLE RDAADPIAPL RDAFALPDGV IYLDGNSLGA RPRAALARAQ
60 70 80 90 100
QVVADEWGEG LIRSWNQSGW FDLPRRLGNK LAPLIGARPD EVVVTDTTSI
110 120 130 140 150
NLFKVLAAAL RVQREDAPER KIIVSETHNF PTDLYIAEGL ADLLRDGYKL
160 170 180 190 200
RLIDAPDELE AALGDDVAVT MLTHVNYKSG HMYDMAAVSA LAHRHGALAV
210 220 230 240 250
WDLAHSAGAV PVDLHAGGAD YAIGCTYKYL NGGPGSPAFV WVSPALRERF
260 270 280 290 300
WQPLSGWWGH ARPFAMESRY DAGAGIGRFL CGTQPITSMA MVECGLDLFA
310 320 330 340 350
QTSMAALRAK SLALTDLFIE LIETRCAGYP LTLATPRDHA VRGSHVSYAH
360 370 380 390 400
PDGFAVMQAL IARGVIGDYR EPHIMRFGFT PLYTRFVDVW DAVEVLRDVL
410
ETGAWQSAQF NERTLVT
Length:417
Mass (Da):45,660
Last modified:July 27, 2011 - v1
Checksum:i20D59ACD45B2C434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002819 Genomic DNA. Translation: AEG69899.1.
RefSeqiWP_014617695.1. NC_017574.1.
YP_006030435.1. NC_017574.1.

Genome annotation databases

EnsemblBacteriaiAEG69899; AEG69899; RSPO_c02606.
GeneIDi12626708.
KEGGirsn:RSPO_c02606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002819 Genomic DNA. Translation: AEG69899.1.
RefSeqiWP_014617695.1. NC_017574.1.
YP_006030435.1. NC_017574.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEG69899; AEG69899; RSPO_c02606.
GeneIDi12626708.
KEGGirsn:RSPO_c02606.

Phylogenomic databases

KOiK01556.
OMAiRFWQPLS.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
BioCyciRSOL1031711:GLJ5-2650-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the plant pathogen Ralstonia solanacearum strain Po82."
    Xu J., Zheng H.J., Liu L., Pan Z.C., Prior P., Tang B., Xu J.S., Zhang H., Tian Q., Zhang L.Q., Feng J.
    J. Bacteriol. 193:4261-4262(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Po82Imported.

Entry informationi

Entry nameiF6G4U4_RALS8
AccessioniPrimary (citable) accession number: F6G4U4
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.