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Protein

Methionine synthase

Gene

Isova_1799

Organism
Isoptericola variabilis (strain 225)
Status
Unreviewed-Annotation score: -Protein predictedi

Functioni

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.UniRule annotation

Catalytic activityi

5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
  • methylcob(III)alaminUniRule annotation

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Methionine synthase (Isova_1799)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetH route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi235ZincUniRule annotation1
Metal bindingi301ZincUniRule annotation1
Metal bindingi302ZincUniRule annotation1
Metal bindingi755Cobalt (cobalamin axial ligand)UniRule annotation1
Binding sitei800CobalaminUniRule annotation1
Binding sitei939S-adenosyl-L-methionineUniRule annotation1
Binding sitei1134S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei1138Cobalamin; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationImported, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesisUniRule annotation
LigandCobalaminUniRule annotation, Cobalt, Metal-bindingUniRule annotation, S-adenosyl-L-methionineUniRule annotation, ZincUniRule annotation

Enzyme and pathway databases

BioCyciIVAR743718:GI39-1832-MONOMER
UniPathwayiUPA00051; UER00081

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine synthaseUniRule annotation (EC:2.1.1.13UniRule annotation)
Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:Isova_1799Imported
OrganismiIsoptericola variabilis (strain 225)Imported
Taxonomic identifieri743718 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesPromicromonosporaceaeIsoptericola
Proteomesi
  • UP000009236 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi743718.Isova_1799

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 316Hcy-bindingInterPro annotationAdd BLAST303
Domaini347 – 610Pterin-bindingInterPro annotationAdd BLAST264
Domaini645 – 739B12-binding N-terminalInterPro annotationAdd BLAST95
Domaini742 – 879B12-bindingInterPro annotationAdd BLAST138
Domaini892 – 1191AdoMet activationInterPro annotationAdd BLAST300

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni832 – 833Cobalamin-bindingUniRule annotation2
Regioni1188 – 1189S-adenosyl-L-methionine bindingUniRule annotation2

Phylogenomic databases

eggNOGiENOG4105C3R Bacteria
COG0646 LUCA
COG1410 LUCA
KOiK00548
OrthoDBiPOG091H030I

Family and domain databases

CDDicd02069 methionine_synthase_B12_BD, 1 hit
Gene3Di1.10.1240.10, 1 hit
3.10.196.10, 2 hits
3.20.20.20, 1 hit
3.20.20.330, 1 hit
InterProiView protein in InterPro
IPR003759 Cbl-bd_cap
IPR006158 Cobalamin-bd
IPR036724 Cobalamin-bd_sf
IPR011005 Dihydropteroate_synth-like
IPR003726 HCY_dom
IPR036589 HCY_dom_sf
IPR033706 Met_synthase_B12-bd
IPR011822 MetH
IPR036594 Meth_synthase_dom
IPR000489 Pterin-binding_dom
IPR004223 VitB12-dep_Met_synth_activ_dom
IPR037010 VitB12-dep_Met_synth_activ_sf
PfamiView protein in Pfam
PF02310 B12-binding, 1 hit
PF02607 B12-binding_2, 1 hit
PF02965 Met_synt_B12, 1 hit
PF00809 Pterin_bind, 1 hit
PF02574 S-methyl_trans, 1 hit
PIRSFiPIRSF000381 MetH, 1 hit
SMARTiView protein in SMART
SM01018 B12-binding_2, 1 hit
SUPFAMiSSF47644 SSF47644, 1 hit
SSF51717 SSF51717, 1 hit
SSF52242 SSF52242, 1 hit
SSF56507 SSF56507, 1 hit
SSF82282 SSF82282, 1 hit
TIGRFAMsiTIGR02082 metH, 1 hit
PROSITEiView protein in PROSITE
PS50974 ADOMET_ACTIVATION, 1 hit
PS51332 B12_BINDING, 1 hit
PS51337 B12_BINDING_NTER, 1 hit
PS50970 HCY, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

F6FWH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSTLDVSAA DARADALLQA MRTRVVVADG AMGTMIQDAD PTLEDYEGHE
60 70 80 90 100
GCNEVLNVSR PDIIRSVHDA YLEVGVDAIE TNTFGANWSN LSDYGIEDRI
110 120 130 140 150
RELARAGAEL ARERADAWST PEHPRWVLGS MGPGTKLPSL GHTTYAHLKA
160 170 180 190 200
TFAEQAAGLL EGGADAILVE TSQDLLQAKA AVNGAREAMA AVGRRVPVIV
210 220 230 240 250
SVTVETTGTM LMGSEIGAAL TTLQALGVDA IGLNCATGPD QMSEHLRHLA
260 270 280 290 300
RHSEVPVACM PNAGLPELGP KGAVYPLTAP ELATAHAQFV EEFSLGLVGG
310 320 330 340 350
CCGTTPEHLR QVVEAVRGRE LTARPVEREN GVASLYSHTD LHQDASYLAI
360 370 380 390 400
GERTNANGSK AFREAMLDGR WDDVVDIARA QTRDGAHLLD VCVDYVGRDG
410 420 430 440 450
VADVREVVSR LASASTLPLV IDSTEPEVIK AGLELVGGRA VVNSVNFEDG
460 470 480 490 500
DGPTSRFARI MPLVKEHGAA VIALTIDEEG QARTAEKKVA IASRLIDTLT
510 520 530 540 550
GDWGMRVDDI IVDALTFPIA TGQEETRRDA IETIEAIREI TRRYPGVHTT
560 570 580 590 600
LGVSNVSFGL NPAARTVLNS VFLHEAVQAG LDSAIVHAAK ILPRTAIPDE
610 620 630 640 650
QWEAAEDLVR DRRRYDDEGN LVHDPLSHLL ELFSGVDSAS LRDQRAAELA
660 670 680 690 700
ALPVGERLER RIIDGARKGL EDDVEEALAL GMKALDIVNT HLLGGMKVVG
710 720 730 740 750
ELFGSGQMQL PFVLQSAEVM KAAVALLEPH MERVAGGPDE TKGTIVLATV
760 770 780 790 800
RGDVHDIGKN LVDIILTNNG YKVVNIGIKQ PISAMIEAAE QHDADVIGMS
810 820 830 840 850
GLLVKSTVVM KENLEELVSR GLEKKWPILL GGAALTRTYV EDDLASMFPG
860 870 880 890 900
TVRYARDAFE GLRLMEPLVR IARGASPDDV GLPPLRKRRH DVVKVTETAP
910 920 930 940 950
EELPARSDVA TDNPVPTPPF WGTRIVKGVQ LADYAAFLDE RATFMGQWGL
960 970 980 990 1000
KPGRGDDGAS YEELVETEGR PRLAEWLERI RTESILDPTV VYGYFPVWSE
1010 1020 1030 1040 1050
GNDLVVGHHE GPDGGSGGPV GTERLRFRFP RQRRDRHLCL ADFVKPRSWA
1060 1070 1080 1090 1100
EETGRFDVLP VQLVTVGDSV SQHTARLFEA NRYREYMELH GLSVQLTEAL
1110 1120 1130 1140 1150
AEFWHARVRD ELGFADEDPS DVEGMFKLAY RGARFSLGYP ACPDMEDRRK
1160 1170 1180 1190
VVELLRPERV GVVLSDELQL HPEQSTDAFV FHHPEAKYFS V
Length:1,191
Mass (Da):129,576
Last modified:July 27, 2011 - v1
Checksum:i31D8F09AAB86D5A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002810 Genomic DNA Translation: AEG44545.1

Genome annotation databases

EnsemblBacteriaiAEG44545; AEG44545; Isova_1799
KEGGiiva:Isova_1799

Similar proteinsi

Entry informationi

Entry nameiF6FWH1_ISOV2
AccessioniPrimary (citable) accession number: F6FWH1
Entry historyiIntegrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health