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F6DSX8 (F6DSX8_DESRL) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein RibBA HAMAP-Rule MF_01283
Gene names
Name:ribBA HAMAP-Rule MF_01283
Ordered Locus Names:Desru_1708
OrganismDesulfotomaculum ruminis (strain ATCC 23193 / DSM 2154 / NCIB 8452 / DL) [Complete proteome] [HAMAP] EMBL AEG59972.1
Taxonomic identifier696281 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum ›

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP-Rule MF_01283 SAAS SAAS017945

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family. HAMAP-Rule MF_01283

In the N-terminal section; belongs to the DHBP synthase family. HAMAP-Rule MF_01283

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding253 – 2575GTP By similarity HAMAP-Rule MF_01283
Nucleotide binding296 – 2983GTP By similarity HAMAP-Rule MF_01283
Region1 – 202202DHBP synthase By similarity HAMAP-Rule MF_01283
Region29 – 302D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region141 – 1455D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region203 – 408206GTP cyclohydrolase II By similarity HAMAP-Rule MF_01283

Sites

Active site3301Proton acceptor; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Active site3321Nucleophile; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Metal binding301Magnesium or manganese 1 By similarity HAMAP-Rule MF_01283
Metal binding301Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding1441Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding2581Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2691Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2711Zinc; catalytic By similarity HAMAP-Rule MF_01283
Binding site341D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site1651D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site2741GTP By similarity HAMAP-Rule MF_01283
Binding site3181GTP By similarity HAMAP-Rule MF_01283
Binding site3531GTP By similarity HAMAP-Rule MF_01283
Binding site3581GTP By similarity HAMAP-Rule MF_01283
Site1271Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283
Site1651Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283

Sequences

Sequence LengthMass (Da)Tools
F6DSX8 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 7474DB770146CA78

FASTA40844,418
        10         20         30         40         50         60 
MTFTFNTIEE AIEDIRAGKM VVVVDDEDRE NEGDLIMAAE KVTTEAVNFM ATHGRGLICM 

        70         80         90        100        110        120 
PIYGERLDQL ALPAMVSNNT DPHGTAFTVS VDAKECSTGI SAVERALTIQ KIMDPDTQPE 

       130        140        150        160        170        180 
DLRRPGHIFP LRAKEGGVLV RTGHTEASVD LSRLAGLKPA AVICEIMKDD GTMARVPELV 

       190        200        210        220        230        240 
EFCKTHNLKL ITIADLIKYR SRHEKFIKRV DSAKMPTKFG DFTAVAYESL LDGKGHIAMV 

       250        260        270        280        290        300 
KGDLSSVEAP LVRVHSECLT GDVFGSCRCD CGDQLAKAMQ AIEKEGTGVL LYMRQEGRGI 

       310        320        330        340        350        360 
GLLNKIRAYK LQDLGADTVE ANLALGFPAD LRDYGIGAQI LVDLGLSKIR LLTNNPRKIA 

       370        380        390        400 
GLEGHGLEVV DRVPIEIAAG QANAKYLSTK KCKLGHLLTV TDQNKKTS

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References

[1]"Complete sequence of Desulfotomaculum ruminis DSM 2154."
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Mikhailova N., Pagani I., Stams A.J.M., Plugge C.M. expand/collapse author list , Muyzer G., Kuever J., Parshina S.N., Ivanova A.E., Nazina T.N., Brambilla E., Spring S., Klenk H.-P., Woyke T.
Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23193 / DSM 2154 / NCIB 8452 / DL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002780 Genomic DNA. Translation: AEG59972.1.
RefSeqYP_004545258.1. NC_015589.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEG59972; AEG59972; Desru_1708.
GeneID10738102.
KEGGdru:Desru_1708.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK14652.

Enzyme and pathway databases

UniPathwayUPA00275; UER00399.
UPA00275; UER00400.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
HAMAPMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF6DSX8_DESRL
AccessionPrimary (citable) accession number: F6DSX8
Entry history
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info