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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus kefiranofaciens (strain ZW3)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei576 – 5761ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciLKEF1033837:GHZJ-196-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:leuSUniRule annotationImported
Ordered Locus Names:WANG_0164Imported
OrganismiLactobacillus kefiranofaciens (strain ZW3)Imported
Taxonomic identifieri1033837 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009228 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi1033837.WANG_0164.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionUniRule annotationAdd
BLAST
Motifi573 – 5775"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

KOiK01869.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F6CDF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNHKVVEKK WQDYWAKHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH
60 70 80 90 100
PEGYTATDIV SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGMDPAVVTK
110 120 130 140 150
QNIANFKEQL NKLGFSYDWD REITTSDPNY YKWTQWVFEQ MYENGLAYEA
160 170 180 190 200
EVPVNWSPDL GTVVANEEVI DGKTERGGYP VYRKNMRQWM LKMTAYADRL
210 220 230 240 250
LEDLDSLDWP EPVKEMQRNW IGRSVGAQVT FKIKGSDKTF DIFTTRPDTL
260 270 280 290 300
FGCSYTVLAP ENKLVQEITT DGHRDEVNAY IKKIESKSDL ERTDLNKDKT
310 320 330 340 350
GVFTGAYAIN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFATK
360 370 380 390 400
FGLPINPVLK GGDISKAAFT EDGPHINSEF LDGLNIKDAK KKMVDWLEDH
410 420 430 440 450
NCGKKKVNYK LRDWDFSRQR YWGEPIPVIH WEDGTTSLVP EDQLPLRLPH
460 470 480 490 500
ATDIKPSGTP ESPLANLTDW VNVVDENGRK GKRETNTMPN WAGSSWYYLR
510 520 530 540 550
YVDPHNDKEL ADYDLLKQWL PVDLYIGGAE HAVRHLLYAR FWHKVLYDLG
560 570 580 590 600
VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDDVIDE YGADSLRMYE
610 620 630 640 650
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDKND
660 670 680 690 700
GELDKVYAET VKKVTEDFDA LHFNTAISQM MVFINAAQKA KSIPREYAEG
710 720 730 740 750
FVKLLAPVAP HMMEEIWQVF GHDESITYAK WPTYDPAKLV ESTVEIMVQV
760 770 780 790 800
NGKLRGKFQA PKDADRDAVQ KQALAIEHVK KFLAGKDIKK VIVVPNKIVN

IVAK
Length:804
Mass (Da):92,005
Last modified:July 27, 2011 - v1
Checksum:i63378F2055D49460
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002764 Genomic DNA. Translation: AEG39859.1.
RefSeqiWP_013853660.1. NC_015602.1.
YP_004561961.1. NC_015602.1.

Genome annotation databases

EnsemblBacteriaiAEG39859; AEG39859; WANG_0164.
KEGGilke:WANG_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002764 Genomic DNA. Translation: AEG39859.1.
RefSeqiWP_013853660.1. NC_015602.1.
YP_004561961.1. NC_015602.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1033837.WANG_0164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEG39859; AEG39859; WANG_0164.
KEGGilke:WANG_0164.

Phylogenomic databases

KOiK01869.

Enzyme and pathway databases

BioCyciLKEF1033837:GHZJ-196-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Lactobacillus kefiranofaciens ZW3."
    Wang Y., Wang J., Ahmed Z., Bai X., Wang J.
    J. Bacteriol. 193:4280-4281(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ZW3Imported.

Entry informationi

Entry nameiF6CDF0_LACKZ
AccessioniPrimary (citable) accession number: F6CDF0
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.