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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Thermoanaerobacterium xylanolyticum (strain ATCC 49914 / DSM 7097 / LX-11)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathway:iglycolysis

This protein is involved in step 3 and 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Pathway:iglycolysis

This protein is involved in step 3 and 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Active sitei128 – 1281Proton acceptorUniRule annotation
Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTXYL858215:GHCH-671-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Thexy_0634Imported
OrganismiThermoanaerobacterium xylanolyticum (strain ATCC 49914 / DSM 7097 / LX-11)Imported
Taxonomic identifieri858215 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium
ProteomesiUP000007239 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi858215.Thexy_0634.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni170 – 1723Substrate bindingUniRule annotation
Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni251 – 2544Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiQRIISIR.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F6BI77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTIGVLTSG GDAPGMNAAI RAVVRCGIYN GLTVKGIMRG YQGLIDDEIE
60 70 80 90 100
DMTLSSVGDI IQRGGTILRT ARSAEFKTKE GRARAAEVLK KHNIEGLVVI
110 120 130 140 150
GGDGSFRGAQ LLSNEHGVNT IGIPGTIDND IPCTDYTIGF DTACNTAIDA
160 170 180 190 200
INKIRDTATS HERANIIEVM GRNAGYIALY AGLAGGAEII IIPEVKFDID
210 220 230 240 250
EVCEKISYGI KRGKLHHIIV LAEGVMSGLE LSKMIQERLP KLDLRHTTLG
260 270 280 290 300
HIQRGGAPTV MDRVMASQMG SRAVELLLEN KSQRIISIRN NQIVDDDIDT
310 320
ALAMKKEFNL KLYELSKILS I
Length:321
Mass (Da):34,785
Last modified:July 27, 2011 - v1
Checksum:i543268309A61BC0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002739 Genomic DNA. Translation: AEF16685.1.
RefSeqiWP_013787434.1. NC_015555.1.

Genome annotation databases

EnsemblBacteriaiAEF16685; AEF16685; Thexy_0634.
KEGGitxy:Thexy_0634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002739 Genomic DNA. Translation: AEF16685.1.
RefSeqiWP_013787434.1. NC_015555.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi858215.Thexy_0634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEF16685; AEF16685; Thexy_0634.
KEGGitxy:Thexy_0634.

Phylogenomic databases

KOiK00850.
OMAiQRIISIR.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciTXYL858215:GHCH-671-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Thermoanaerobacterium xylanolyticum LX-11."
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Lu M., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Hemme C., Woyke T.
    Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiF6BI77_THEXL
AccessioniPrimary (citable) accession number: F6BI77
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.