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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Delftia sp. (strain Cs1-4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi106Magnesium; catalyticUniRule annotation1
Sitei107Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei131Proton acceptorUniRule annotation1
Binding sitei166Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei225SubstrateUniRule annotation1
Binding sitei269Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 108ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSP742013:GH2F-1374-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:DelCs14_1360Imported
OrganismiDelftia sp. (strain Cs1-4)Imported
Taxonomic identifieri742013 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
Proteomesi
  • UP000009225 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 300PFKInterPro annotationAdd BLAST299

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131Substrate bindingUniRule annotation3
Regioni173 – 175Substrate bindingUniRule annotation3
Regioni275 – 278Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiPEISYTI.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F6B1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVGVLTGGG DCPGLNAVIR AVTKSLINHG QCEVLGIADG FEGLMLDPPG
60 70 80 90 100
VKPLGWDEVS GILHVGGTIL GTSNSANPLR DAATLEHVGR NVRALGLDVV
110 120 130 140 150
VAIGGDGTMS LAHGLAEQVG LTCVGVPKTI DNDIANCERS FGFDTAVATA
160 170 180 190 200
TEALRRIEST ANSHHRVMIV ETMGRHAGWL ALEAGIAGAA DVILLPEIDY
210 220 230 240 250
DLQAIIDFCR VREQRQRYTI ICIGEGAKES GASLTVRERI EQSPDPVRLG
260 270 280 290 300
GVGHVLRERL QPHLRSEVRT TVLGHVQRGG DPTPFDRVLA TQYGHHAAQL
310 320 330 340 350
VLSGRFGRMV TLQGGEIGSV EISRVANTQR TVPLDHGLLT MARDIGICLG
360
EPCKQDARPA AAGSH
Length:365
Mass (Da):38,623
Last modified:July 27, 2011 - v1
Checksum:iDA287E37C902728B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002735 Genomic DNA. Translation: AEF88392.1.
RefSeqiWP_013801002.1. NC_015563.1.

Genome annotation databases

EnsemblBacteriaiAEF88392; AEF88392; DelCs14_1360.
KEGGidel:DelCs14_1360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002735 Genomic DNA. Translation: AEF88392.1.
RefSeqiWP_013801002.1. NC_015563.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEF88392; AEF88392; DelCs14_1360.
KEGGidel:DelCs14_1360.

Phylogenomic databases

KOiK00850.
OMAiPEISYTI.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciDSP742013:GH2F-1374-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF6B1J1_DELSC
AccessioniPrimary (citable) accession number: F6B1J1
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.