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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Delftia sp. (strain Cs1-4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
Sitei107 – 1071Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei131 – 1311Proton acceptorUniRule annotation
Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Binding sitei269 – 2691Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi105 – 1084ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSP742013:GH2F-1384-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotationSAAS annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:DelCs14_1360Imported
OrganismiDelftia sp. (strain Cs1-4)Imported
Taxonomic identifieri742013 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
ProteomesiUP000009225 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1313Substrate bindingUniRule annotation
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni275 – 2784Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F6B1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVGVLTGGG DCPGLNAVIR AVTKSLINHG QCEVLGIADG FEGLMLDPPG
60 70 80 90 100
VKPLGWDEVS GILHVGGTIL GTSNSANPLR DAATLEHVGR NVRALGLDVV
110 120 130 140 150
VAIGGDGTMS LAHGLAEQVG LTCVGVPKTI DNDIANCERS FGFDTAVATA
160 170 180 190 200
TEALRRIEST ANSHHRVMIV ETMGRHAGWL ALEAGIAGAA DVILLPEIDY
210 220 230 240 250
DLQAIIDFCR VREQRQRYTI ICIGEGAKES GASLTVRERI EQSPDPVRLG
260 270 280 290 300
GVGHVLRERL QPHLRSEVRT TVLGHVQRGG DPTPFDRVLA TQYGHHAAQL
310 320 330 340 350
VLSGRFGRMV TLQGGEIGSV EISRVANTQR TVPLDHGLLT MARDIGICLG
360
EPCKQDARPA AAGSH
Length:365
Mass (Da):38,623
Last modified:July 27, 2011 - v1
Checksum:iDA287E37C902728B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002735 Genomic DNA. Translation: AEF88392.1.
RefSeqiWP_013801002.1. NC_015563.1.
YP_004486747.1. NC_015563.1.

Genome annotation databases

EnsemblBacteriaiAEF88392; AEF88392; DelCs14_1360.
KEGGidel:DelCs14_1360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002735 Genomic DNA. Translation: AEF88392.1.
RefSeqiWP_013801002.1. NC_015563.1.
YP_004486747.1. NC_015563.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEF88392; AEF88392; DelCs14_1360.
KEGGidel:DelCs14_1360.

Phylogenomic databases

KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciDSP742013:GH2F-1384-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Delftia sp. Cs1-4."
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Teshima H., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Bradfield C., Sheety A.
    , Cheng S., Chain P., Denef V., Hickey W., Woyke T.
    Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Cs1-4Imported.

Entry informationi

Entry nameiF6B1J1_DELSC
AccessioniPrimary (citable) accession number: F6B1J1
Entry historyi
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.