F6A8G7 (F6A8G7_PSEF1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit HAMAP-Rule MF_01031
EC=4.2.1.33 HAMAP-Rule MF_01031

Alternative name(s):
Alpha-IPM isomerase HAMAP-Rule MF_01031
Isopropylmalate isomerase HAMAP-Rule MF_01031

Gene names

Name:	leuD HAMAP-Rule MF_01031
Ordered Locus Names:	Psefu_3074 EMBL AEF23038.1

Organism

Pseudomonas fulva (strain 12-X) [Complete proteome] [HAMAP] EMBL AEF23038.1

Taxonomic identifier

743720 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas fulva

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. SAAS SAAS000573 HAMAP-Rule MF_01031
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. SAAS SAAS004431 HAMAP-Rule MF_01031
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. SAAS SAAS004431 HAMAP-Rule MF_01031
Subunit structure	Heterodimer of LeuC and LeuD By similarity. SAAS SAAS000573 HAMAP-Rule MF_01031
Sequence similarities	Belongs to the LeuD family. LeuD type 1 subfamily. HAMAP-Rule MF_01031

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis SAAS SAAS004431 HAMAP-Rule MF_01031
Molecular function	Lyase SAAS SAAS004431 HAMAP-Rule MF_01031
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	F6A8G7 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: 2B521DDBC18A2E28	FASTA	215	24,328
	10 20 30 40 50 60 MKAFTQHTGL VCPLDRANVD TDQIIPKQFL KSIKRTGFGP NLFDEWRYLD VGQPNQDNSK 70 80 90 100 110 120 RPINKDFVLN FPRYQGASVL LARENFGCGS SREHAPWALD EYGFRTVIAP SFADIFFNNS 130 140 150 160 170 180 FKNGLLPIIL KAEEVDALFE QAEASEGYQL TVDLEAQTVT RPDGVQYSFE VDAFRKHCLL 190 200 210 NGLDDIGLTL QDAEAIRAFE VGYQQRQPWL FGAVK

References

[1]

"Complete sequence of Pseudomonas fulva 12-X." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Pagani I., Davenport K., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Lcollab F.I., Woyke T. Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12-X.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002727 Genomic DNA. Translation: AEF23038.1.
RefSeq	YP_004475132.1. NC_015556.1.
3D structure databases
ModBase	Search...
MobiDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEF23038; AEF23038; Psefu_3074.
GeneID	10657970.
KEGG	pfv:Psefu_3074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01704.
OMA	AFTTHTG.
Enzyme and pathway databases
BioCyc	PFUL743720:GHQV-3129-MONOMER.
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.20.19.10. 1 hit.
HAMAP	MF_01031. LeuD_type1.
InterPro	IPR004431. 3-IsopropMal_deHydase_ssu. IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. SSF52016. 1 hit.
TIGRFAMs	TIGR00171. leuD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F6A8G7_PSEF1

Accession

Primary (citable) accession number: F6A8G7

Entry history

Integrated into UniProtKB/TrEMBL:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)