leuD

Functionⁱ

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotationSAAS annotation

Catalytic activityⁱ

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotationSAAS annotation

Pathwayⁱ: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

2-isopropylmalate synthase (leuA), 2-isopropylmalate synthase (leuA)
3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
3-isopropylmalate dehydrogenase (leuB)
Branched-chain-amino-acid aminotransferase (Psefu_1539)

This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functionⁱ

3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP

Complete GO annotation...

GO - Biological processⁱ

leucine biosynthetic process Source: UniProtKB-HAMAP

Complete GO annotation...

Keywordsⁱ

Molecular function	LyaseUniRule annotationSAAS annotation
Biological process	Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayⁱ	UPA00048; UER00071.

UniPathwayⁱ

UPA00048; UER00071.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 3-isopropylmalate dehydratase small subunitUniRule annotation (EC:4.2.1.33UniRule annotation) Alternative name(s): Alpha-IPM isomeraseUniRule annotation Short name: IPMIUniRule annotation Isopropylmalate isomeraseUniRule annotation
Gene namesⁱ	Name:leuDUniRule annotation Ordered Locus Names:Psefu_3074Imported
Organismⁱ	Pseudomonas fulva (strain 12-X)Imported
Taxonomic identifierⁱ	743720 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas fulva
Proteomesⁱ	UP000000686 Componentⁱ: Chromosome

Subcellular locationⁱ

GO - Cellular componentⁱ

3-isopropylmalate dehydratase complex Source: InterPro

Complete GO annotation...

Interactionⁱ

Subunit structureⁱ

Heterodimer of LeuC and LeuD.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGⁱ	743720.Psefu_3074.

STRINGⁱ

743720.Psefu_3074.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 132	Aconitase_CInterPro annotationAdd BLAST		132

Sequence similaritiesⁱ

Belongs to the LeuD family. LeuD type 1 subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105MQS. Bacteria. COG0066. LUCA.
KEGG Orthology (KO) More...KOⁱ	K01704.
OMAⁱ	AFTTHTG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02FJ.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01577. IPMI_Swivel. 1 hit.
Gene3Dⁱ	3.20.19.10. 1 hit.
HAMAPⁱ	MF_01031. LeuD_type1. 1 hit.
InterProⁱ	View protein in InterPro IPR004431. 3-IsopropMal_deHydase_ssu. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. IPR033940. IPMI_Swivel.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00694. Aconitase_C. 1 hit.
SUPFAMⁱ	SSF52016. SSF52016. 1 hit.
TIGRFAMsⁱ	TIGR00171. leuD. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

F6A8G7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKAFTQHTGL VCPLDRANVD TDQIIPKQFL KSIKRTGFGP NLFDEWRYLD 
        60         70         80         90        100
VGQPNQDNSK RPINKDFVLN FPRYQGASVL LARENFGCGS SREHAPWALD 
       110        120        130        140        150
EYGFRTVIAP SFADIFFNNS FKNGLLPIIL KAEEVDALFE QAEASEGYQL 
       160        170        180        190        200
TVDLEAQTVT RPDGVQYSFE VDAFRKHCLL NGLDDIGLTL QDAEAIRAFE 
       210 
VGYQQRQPWL FGAVK

Length:215

Mass (Da):24,328

Last modified:July 27, 2011 - v1

Checksum:ⁱ2B521DDBC18A2E28

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP002727 Genomic DNA. Translation: AEF23038.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_013792165.1. NC_015556.1.

Genome annotation databases

EnsemblBacteriaⁱ	AEF23038; AEF23038; Psefu_3074.
GeneIDⁱ	31810842.
KEGGⁱ	pfv:Psefu_3074.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP002727 Genomic DNA. Translation: AEF23038.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_013792165.1. NC_015556.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	743720.Psefu_3074.

STRINGⁱ

743720.Psefu_3074.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AEF23038; AEF23038; Psefu_3074.
GeneIDⁱ	31810842.
KEGGⁱ	pfv:Psefu_3074.

Phylogenomic databases

eggNOGⁱ	ENOG4105MQS. Bacteria. COG0066. LUCA.
KEGG Orthology (KO) More...KOⁱ	K01704.
OMAⁱ	AFTTHTG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02FJ.

Enzyme and pathway databases

UniPathwayⁱ	UPA00048; UER00071.

UniPathwayⁱ

UPA00048; UER00071.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01577. IPMI_Swivel. 1 hit.
Gene3Dⁱ	3.20.19.10. 1 hit.
HAMAPⁱ	MF_01031. LeuD_type1. 1 hit.
InterProⁱ	View protein in InterPro IPR004431. 3-IsopropMal_deHydase_ssu. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. IPR033940. IPMI_Swivel.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00694. Aconitase_C. 1 hit.
SUPFAMⁱ	SSF52016. SSF52016. 1 hit.
TIGRFAMsⁱ	TIGR00171. leuD. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	F6A8G7_PSEF1
Accessionⁱ	F6A8G7Primary (citable) accession number: F6A8G7
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	July 27, 2011
	Last sequence update:	July 27, 2011
	Last modified:	June 7, 2017
	This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteomeImported

UniProtKB - F6A8G7 (F6A8G7_PSEF1)

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3-isopropylmalate dehydratase small subunit

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Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: L-leucine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Subunit structureⁱ

Protein-protein interaction databases

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - F6A8G7 (F6A8G7_PSEF1)

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3-isopropylmalate dehydratase small subunit

leuD

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-leucine biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Functionⁱ

Pathwayⁱ: L-leucine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ