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F5YW57 (F5YW57_MYCSD) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase HAMAP-Rule MF_00197
Short name=DAP epimerase HAMAP-Rule MF_00197
EC=5.1.1.7 HAMAP-Rule MF_00197
Gene names
Name:dapF HAMAP-Rule MF_00197 EMBL AEF36468.1
Ordered Locus Names:JDM601_2468 EMBL AEF36468.1
OrganismMycobacterium sp. (strain JDM601) [Complete proteome] [HAMAP]
Taxonomic identifier875328 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197 SAAS SAAS001653

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197 SAAS SAAS001653

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197 SAAS SAAS001653.

Sequence similarities

Belongs to the diaminopimelate epimerase family. HAMAP-Rule MF_00197

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region87 – 893Substrate binding By similarity HAMAP-Rule MF_00197
Region217 – 2182Substrate binding By similarity HAMAP-Rule MF_00197
Region227 – 2282Substrate binding By similarity HAMAP-Rule MF_00197

Sites

Active site871Proton donor/acceptor By similarity HAMAP-Rule MF_00197
Active site2261Proton donor/acceptor By similarity HAMAP-Rule MF_00197
Binding site111Substrate By similarity HAMAP-Rule MF_00197
Binding site441Substrate By similarity HAMAP-Rule MF_00197
Binding site781Substrate By similarity HAMAP-Rule MF_00197
Binding site1631Substrate By similarity HAMAP-Rule MF_00197
Binding site1991Substrate By similarity HAMAP-Rule MF_00197
Site1651Important for catalytic activity By similarity HAMAP-Rule MF_00197
Site2171Important for catalytic activity By similarity HAMAP-Rule MF_00197

Amino acid modifications

Disulfide bond87 ↔ 226Size: 249-333 By similarity HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
F5YW57 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: B37811F78D2DCC71

FASTA28829,326
        10         20         30         40         50         60 
MLFAKGHGTQ NDFVVLPDLG VELDLTAAAV SALCDRRRGL GADGVLRVTT AGAAVTAGVL 

        70         80         90        100        110        120 
DQLPEGVSAG DWFMDYRNAD GSVAEMCGNG VRVFAHYLRA AGLESADEFV VGSLAGPRPI 

       130        140        150        160        170        180 
TVHHADDVRA EVTVDMGKAN TLGPGEAVVG GRRFAGLAVD VGNPHLACVD PALSAAELAA 

       190        200        210        220        230        240 
LDVAAPVSFD RGQFPNGVNV EVLTAARDGA VSMRVHERGV GETRSCGTGT VAAAVAALAD 

       250        260        270        280 
AGASTGTLRV DIPGGQVRVI VTEATSYLRG PSELVARGDI AAGWWSAQ

« Hide

References

[1]"Complete gnome sequence of a novel clinical isolate, the nontuberculous Mycobacterium strain JDM601."
Zhang Z.Y., Sun Z.Q., Wang Z.L., Wen Z.L., Sun Q.W., Zhu Z.Q., Song Y.Z., Zhao J.W., Wang H.H., Zhang S.L., Guo X.K.
J. Bacteriol. 193:4300-4301(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JDM601 EMBL AEF36468.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002329 Genomic DNA. Translation: AEF36468.1.
RefSeqYP_004523722.1. NC_015576.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEF36468; AEF36468; JDM601_2468.
GeneID10684497.
KEGGmjd:JDM601_2468.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01778.

Enzyme and pathway databases

BioCycMSP875328:GHLX-2469-MONOMER.
UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF5YW57_MYCSD
AccessionPrimary (citable) accession number: F5YW57
Entry history
Integrated into UniProtKB/TrEMBL: July 27, 2011
Last sequence update: July 27, 2011
Last modified: April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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