F5XPH8 (F5XPH8_MICPN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
NADP(+)-dependent isocitrate dehydrogenase EMBL BAK34286.1
EC=1.1.1.42 EMBL BAK34286.1

Gene names

Name:	icd EMBL BAK34286.1
Ordered Locus Names:	MLP_12720 EMBL BAK34286.1

Organism

Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) [Complete proteome] [HAMAP] EMBL BAK34286.1

Taxonomic identifier

1032480 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Propionibacteriaceae › Microlunatus › Microlunatus phosphovorus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRSR PIRSR009407-3

Ontologies

Keywords
Ligand	Magnesium PIRSR PIRSR009407-3 Metal-binding PIRSR PIRSR009407-3
Molecular function	Oxidoreductase EMBL BAK34286.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	134 – 141	8	Substrate binding By similarity PIRSR PIRSR009407-2
Sites
	Metal binding	350	1	Magnesium or manganese By similarity PIRSR PIRSR009407-3
	Metal binding	548	1	Magnesium or manganese By similarity PIRSR PIRSR009407-3
	Metal binding	552	1	Magnesium or manganese By similarity PIRSR PIRSR009407-3
	Binding site	147	1	Substrate By similarity PIRSR PIRSR009407-2
	Binding site	547	1	Substrate By similarity PIRSR PIRSR009407-2
	Site	257	1	Critical for catalysis By similarity PIRSR PIRSR009407-1
	Site	420	1	Critical for catalysis By similarity PIRSR PIRSR009407-1

Sequences

	Sequence		Length	Mass (Da)
	F5XPH8 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: FF530C866D0676D7	FASTA	740	79,570
	10 20 30 40 50 60 MVQSSARIVY TRTDEAPLLA TYSLLPIIAA YAAKAGVDIE SRDISLAARI LAQFPERLGA 70 80 90 100 110 120 DQQVPDALAE LGALAKTPEA NIIKLPNISA SIPQLKAAIR ELQEKGFDLP DYPDDPKSDE 130 140 150 160 170 180 DRSIRARYDK VKGSAVNPVL REGNSDRRAP LSVKNYARKH PHRMGAWSGD SKTNVAHMTA 190 200 210 220 230 240 DDFRSNEKSV VIPADTSLRI ALVEEAGAET VLRESVPVLA GEVVDATVLR VAALRAFLTE 250 260 270 280 290 300 QIARAKAENV LLSVHLKATM MKVSDPIIFG HAVRAFFPEV FETYGDALAT AGLSPNDGLG 310 320 330 340 350 360 AILAGLDALP EGPEIKAAID RGFAEGPALA MVDSDRGISN LHVPSDVIVD ASMPAMIRTS 370 380 390 400 410 420 GHMWGPDGAE ADTLAVLPDS SYAPIYQVVI DDCRANGAFD PATMGSVPNV GLMAQAAEEY 430 440 450 460 470 480 GSHDKTFEIP AEGVVKVTDS EGNVVLEHRV SQGDIWRMCQ AKDVPIRDWI KLAVSRARAT 490 500 510 520 530 540 GSPAVFWLDE TRAHDANLIA KINETLPEYD TDGLEIKILS PADAMAYSLE RIRRGEDTIS 550 560 570 580 590 600 VTGNVLRDYL TDLFPIMELG TSAKMLSVVP LINGGGLFET GAGGSAPKHV QQLLKEDYLR 610 620 630 640 650 660 WDSLGEFLAL AVSLEHLAQS TGNKRAQVLA DTLDRATGTF LDENKSPARR VGSIDNRGSH 670 680 690 700 710 720 FYLALYWAEE LAAQTDDAEL AAEFATLAAA LREQEQTISD ELIAVQGAPV DIGGYYRPDD 730 740 DLTSAVMRPS ATFNAAIAAL

References

[1]

"Whole genome sequence of Microlunatus phosphovorus NM-1." Hosoyama A., Sasaki K., Harada T., Igarashi R., Kawakoshi A., Sasagawa M., Fukada J., Nakamura S., Katano Y., Hanada S., Kamagata Y., Nakamura N., Yamazaki S., Fujita N. Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP012204 Genomic DNA. Translation: BAK34286.1.
RefSeq	YP_004571689.1. NC_015635.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAK34286; BAK34286; MLP_12720.
GeneID	10767775.
KEGG	mph:MLP_12720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00031.
Enzyme and pathway databases
BioCyc	MPHO1032480:GHBY-1284-MONOMER.
Family and domain databases
Gene3D	3.40.718.10. 3 hits.
InterPro	IPR004436. Isocitrate_DH_NADP_mono. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
Pfam	PF03971. IDH. 1 hit. [Graphical view]
PIRSF	PIRSF009407. IDH_monmr. 1 hit.
TIGRFAMs	TIGR00178. monomer_idh. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F5XPH8_MICPN

Accession

Primary (citable) accession number: F5XPH8

Entry history

Integrated into UniProtKB/TrEMBL:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)