F5HG51 (PRIM_HCMVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase UL70
EC=2.7.7.-

Gene names

Name:

UL70

Organism

Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5) [Reference proteome]

Taxonomic identifier

295027 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › Human cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The UL70 subunit bears the primase activity By similarity.
Subunit structure	Associates with the helicase-primase complex composed of the primase UL70, the helicase UL105 and the primase-associated factor UL102. Interacts with host SNAPIN By similarity.
Subcellular location	Host nucleus By similarity.
Sequence similarities	Belongs to the herpesviridae UL52 family.

Ontologies

Keywords
Biological process	DNA replication Host-virus interaction
Cellular component	Host nucleus
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: GOC virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA primase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	DNA primase UL70		PRO_0000416450
Regions
	Compositional bias	585 – 588	4	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	F5HG51 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 0818168F70714FAC	FASTA	946	107,842
	10 20 30 40 50 60 MTLVLFATEY DSAHIVANVL SQTPTDHCVF PLLVKHQVSR RVYFCLQTQK CSDSRRVAPV 70 80 90 100 110 120 FAVNNETLQL SRYLAARQPI PLSALIASLD EAETRPLYRH LFRTPVLSPE HGGEVREFKH 130 140 150 160 170 180 LVYFHHAAVL RHLNQVFLCP TSPSWFISVF GHTEGQVLLT MAYYLFEGQY STISTVEEYV 190 200 210 220 230 240 RSFCTRDLGT IIPTHASMGE FARLLLGSPF RQRVSAFVAY AVARNRRDYT ELEQVDTQIN 250 260 270 280 290 300 AFRERARLPD TVCVHYVYLA YRTALARARL LEYRRVVAYD ADAAPEAQCT REPGFLGRRL 310 320 330 340 350 360 STELLDVMQK YFSLDNFLHD YVETHLLRLD ESPHSATSPH GLGLAGYGGR IDGTHLAGFF 370 380 390 400 410 420 GTSTQLARQL ERINTLSESV FSPLERSLSG LLRLCASLRT AQTYTTGTLT RYSQRRYLLP 430 440 450 460 470 480 EPALAPLLER PLPVYRVHLP NDQHVFCAVA SETWHRSLFP RDLLRHVPDS RFSDEALTET 490 500 510 520 530 540 VWLHDDDVAS TSPETQFYYT RHEVFNERLP VFNFVADFDL RLRDGVSGLA RHTVFELCRG 550 560 570 580 590 600 LRRVWMTVWA SLFGYTHPDR HPVYFFKSAC PPNSVPVDAA GAPFDDDDYL DYRDERDTEE 610 620 630 640 650 660 DEDGKEDKNN VPGNGVFQKT TSSVDTSPPY CRCKGKLGLR IITPFPACTI AVHPSVLRAV 670 680 690 700 710 720 AQVLNHAVCL DAELHTLLDP ISHPESSLDT GIYHHGRSVR LPYMYKMDQD DGYFMHRRLL 730 740 750 760 770 780 PLFIVPDAYR EHPLGFVRAQ LDLRNLLHHH PPHDLPALPL SPPPRVILSV RDKICPSTEA 790 800 810 820 830 840 NFIETRSLNV TRYRRRGLTE VLAYHLYGGD GATAAAISDT DLQRLVVTRV WPPLLEHLTQ 850 860 870 880 890 900 HYEPHVSEQF TAPHVLLFQP HGACCVAVKR RDGARTRDFR CLNYTHRNPQ ETVQVFIDLR 910 920 930 940 TEHSYALWAS LWSRCFTKKC HSNAKNVHIS IKIRPPDAPM PPATAV

References

[1]

"Genetic content of wild-type human cytomegalovirus." Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L., Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C., Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J. J. Gen. Virol. 85:1301-1312(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY446894 Genomic DNA. Translation: AAR31624.1.
RefSeq	YP_081518.1. NC_006273.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3077484.
Family and domain databases
InterPro	IPR004340. DNA_primase_UL52/UL70_Herpvir. [Graphical view]
Pfam	PF03121. Herpes_UL52. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIM_HCMVM

Accession

Primary (citable) accession number: F5HG51

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2012
Last sequence update:	June 28, 2011
Last modified:	October 3, 2012
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program