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Protein

DNA primase

Gene

UL70

Organism
Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Essential component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase initiates primer synthesis and thereby produces large amount of short RNA primers on the lagging strand that the polymerase elongates using dNTPs.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei517 – 5171Essential for primase activityUniRule annotation
Sitei519 – 5191Essential for primase activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri881 – 92040CHC2-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

DNA replication, Host-virus interaction

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:UL70
OrganismiHuman cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri295027 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000938 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

  • Note: Requires the presence of the primase associated factor to properly localize in the host cell nucleus.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946DNA primasePRO_0000416450Add
BLAST

Interactioni

Subunit structurei

Associates with the helicase and the primase-associated factor to form the helicase-primase factor.UniRule annotation

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi585 – 5884Poly-Asp

Sequence similaritiesi

Belongs to the herpesviridae DNA primase family.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri881 – 92040CHC2-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

HAMAPiMF_04011. HSV_PRIM. 1 hit.
InterProiIPR033685. HSV_PRIM.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F5HG51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVLFATEY DSAHIVANVL SQTPTDHCVF PLLVKHQVSR RVYFCLQTQK
60 70 80 90 100
CSDSRRVAPV FAVNNETLQL SRYLAARQPI PLSALIASLD EAETRPLYRH
110 120 130 140 150
LFRTPVLSPE HGGEVREFKH LVYFHHAAVL RHLNQVFLCP TSPSWFISVF
160 170 180 190 200
GHTEGQVLLT MAYYLFEGQY STISTVEEYV RSFCTRDLGT IIPTHASMGE
210 220 230 240 250
FARLLLGSPF RQRVSAFVAY AVARNRRDYT ELEQVDTQIN AFRERARLPD
260 270 280 290 300
TVCVHYVYLA YRTALARARL LEYRRVVAYD ADAAPEAQCT REPGFLGRRL
310 320 330 340 350
STELLDVMQK YFSLDNFLHD YVETHLLRLD ESPHSATSPH GLGLAGYGGR
360 370 380 390 400
IDGTHLAGFF GTSTQLARQL ERINTLSESV FSPLERSLSG LLRLCASLRT
410 420 430 440 450
AQTYTTGTLT RYSQRRYLLP EPALAPLLER PLPVYRVHLP NDQHVFCAVA
460 470 480 490 500
SETWHRSLFP RDLLRHVPDS RFSDEALTET VWLHDDDVAS TSPETQFYYT
510 520 530 540 550
RHEVFNERLP VFNFVADFDL RLRDGVSGLA RHTVFELCRG LRRVWMTVWA
560 570 580 590 600
SLFGYTHPDR HPVYFFKSAC PPNSVPVDAA GAPFDDDDYL DYRDERDTEE
610 620 630 640 650
DEDGKEDKNN VPGNGVFQKT TSSVDTSPPY CRCKGKLGLR IITPFPACTI
660 670 680 690 700
AVHPSVLRAV AQVLNHAVCL DAELHTLLDP ISHPESSLDT GIYHHGRSVR
710 720 730 740 750
LPYMYKMDQD DGYFMHRRLL PLFIVPDAYR EHPLGFVRAQ LDLRNLLHHH
760 770 780 790 800
PPHDLPALPL SPPPRVILSV RDKICPSTEA NFIETRSLNV TRYRRRGLTE
810 820 830 840 850
VLAYHLYGGD GATAAAISDT DLQRLVVTRV WPPLLEHLTQ HYEPHVSEQF
860 870 880 890 900
TAPHVLLFQP HGACCVAVKR RDGARTRDFR CLNYTHRNPQ ETVQVFIDLR
910 920 930 940
TEHSYALWAS LWSRCFTKKC HSNAKNVHIS IKIRPPDAPM PPATAV
Length:946
Mass (Da):107,842
Last modified:June 28, 2011 - v1
Checksum:i0818168F70714FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY446894 Genomic DNA. Translation: AAR31624.1.
RefSeqiYP_081518.1. NC_006273.2.

Genome annotation databases

GeneIDi3077484.
KEGGivg:3077484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY446894 Genomic DNA. Translation: AAR31624.1.
RefSeqiYP_081518.1. NC_006273.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3077484.
KEGGivg:3077484.

Family and domain databases

HAMAPiMF_04011. HSV_PRIM. 1 hit.
InterProiIPR033685. HSV_PRIM.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRIM_HCMVM
AccessioniPrimary (citable) accession number: F5HG51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 28, 2011
Last modified: September 7, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.