F5HEU0 (US03_HCMVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Membrane glycoprotein US3

Gene names

Name:

US3

Organism

Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5) [Reference proteome]

Taxonomic identifier

295027 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › Human cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Retains, but does not degrade MHC class I heterodimers in the endoplasmic reticulum during the immediate-early period of virus infection, thereby impairing their transport and maturation. Forms a complex with beta-2-microglobulin-associated class I heavy chains, which accumulate in the ER. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.
Subunit structure	Monomer By similarity.
Subcellular location	Host endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Developmental stage	Expressed at immediate-early period of virus infection and at reduced levels at early-late times.
Post-translational modification	The signal sequence is not cleaved By similarity. N-glycosylated; mostly exists in a high-mannose form.
Sequence similarities	Belongs to the cytomegalovirus US2 family. Contains 1 Ig-like H-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Viral immunoevasion
Cellular component	Host endoplasmic reticulum Host membrane Membrane
Developmental stage	Early protein
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Ligand	Lectin Mannose-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	evasion or tolerance by virus of host immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	mannose binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 186	166	Membrane glycoprotein US3		PRO_0000416721
Regions
	Topological domain	21 – 160	140	Lumenal Potential
	Transmembrane	161 – 181	21	Helical; Potential
	Topological domain	182 – 186	5	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	44 ↔ 129		By similarity

Sequences

	Sequence		Length	Mass (Da)
	F5HEU0 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 34D58C17BE5EA06B	FASTA	186	21,556
	10 20 30 40 50 60 MKPVLVLAIL AVLFLRLADS VPRPLNVVVS EIKSAHFRVE ENQCWFHMGM LYFKGRMSGN 70 80 90 100 110 120 FTKKHFVNVG IVSQSYMDRL QVSGEQYHHD ERGAYFEWNI GGYPVSHTVD MVDITLSTRW 130 140 150 160 170 180 GDPKKYAACV PQVRMDYSSQ TINWYLQRSM RDDNWGLLFR TLLVYLFSLV VLVLLTVGVS ARLRFI

References

[1]

"Genetic content of wild-type human cytomegalovirus." Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L., Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C., Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J. J. Gen. Virol. 85:1301-1312(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY446894 Genomic DNA. Translation: AAR31694.1.
RefSeq	YP_081590.1. NC_006273.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3077532.
Family and domain databases
InterPro	IPR009237. Herpes_US2/US3. IPR014756. Ig_E-set. [Graphical view]
Pfam	PF05963. Cytomega_US3. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...

Entry information

Entry name

US03_HCMVM

Accession

Primary (citable) accession number: F5HEU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	June 28, 2011
Last modified:	March 6, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program