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Protein

Thymidine kinase

Gene

TK

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei287 – 2871Proton acceptorUniRule annotation
Binding sitei325 – 3251SubstrateUniRule annotation
Binding sitei415 – 4151ATPUniRule annotation
Binding sitei421 – 4211SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi260 – 2678ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
Ordered Locus Names:ORF21
OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Taxonomic identifieri868565 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000942 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Thymidine kinasePRO_0000423759Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF5HB62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

F5HB62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGGFGADS VGRGGEKASV TRGGRWDLGS SDDESSTSTT STDMDDLPEE
60 70 80 90 100
RKPLTGKSVK TSYIYDVPTV PTSKPWHLMH DNSLYATPRF PPRPLIRHPS
110 120 130 140 150
EKGSIFASRL SATDDDSGDY APMDRFAFQS PRVCGRPPLP PPNHPPPATR
160 170 180 190 200
PADASMGDVG WADLQGLKRT PKGFLKTSTK GGSLKARGRD VGDRLRDGGF
210 220 230 240 250
AFSPRGVKSA IGQNIKSWLG IGESSATAVP VTTQLMVPVH LIRTPVTVDY
260 270 280 290 300
RNVYLLYLEG VMGVGKSTLV NAVCGILPQE RVTSFPEPMV YWTRAFTDCY
310 320 330 340 350
KEISHLMKSG KAGDPLTSAK IYSCQNKFSL PFRTNATAIL RMMQPWNVGG
360 370 380 390 400
GSGRGTHWCV FDRHLLSPAV VFPLMHLKHG RLSFDHFFQL LSIFRATEGD
410 420 430 440 450
VVAILTLSSA ESLRRVRARG RKNDGTVEQN YIRELAWAYH AVYCSWIMLQ
460 470 480 490 500
YITVEQMVQL CVQTTNIPEI CFRSVRLAHK EETLKNLHEQ SMLPMITGVL
510 520 530 540 550
DPVRHHPVVI ELCFCFFTEL RKLQFIVADA DKFHDDVCGL WTEIYRQILS
560 570 580
NPAIKPRAIN WPALESQSKA VNHLEETCRV
Length:580
Mass (Da):64,537
Last modified:June 28, 2011 - v1
Checksum:iC34E41D6B181DAFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148805 Genomic DNA. Translation: ABD28872.1.
RefSeqiYP_001129374.1. NC_009333.1.

Genome annotation databases

GeneIDi4961484.
KEGGivg:4961484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148805 Genomic DNA. Translation: ABD28872.1.
RefSeqiYP_001129374.1. NC_009333.1.

3D structure databases

ProteinModelPortaliF5HB62.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4961484.
KEGGivg:4961484.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification of a spliced gene from Kaposi's sarcoma-associated herpesvirus encoding a protein with similarities to latent membrane proteins 1 and 2A of Epstein-Barr virus."
    Glenn M., Rainbow L., Aurade F., Davison A., Schulz T.F.
    J. Virol. 73:6953-6963(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview."
    Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.
    J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_HHV8P
AccessioniPrimary (citable) accession number: F5HB62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 28, 2011
Last modified: July 6, 2016
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.