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Protein

Alpha-conotoxin Mr1.7a

Gene
N/A
Organism
Conus marmoreus (Marble cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-conotoxin MrIC: acts as a co-agonist with PNU (an alpha-7 nAChR-selective allosteric modulator) at the endogenous alpha-7/CHRNA7 nicotinic acetylcholine receptors (nAChR) when tested in human SH-SY5Y neuroblastoma cells. Is the third alpha-conotoxin that acts as an agonist (after alpha-conotoxin SrIA/SrIB). Also acts as an antagonist at human alpha-7 nAChRs heterologously expressed in Xenopus oocytes.1 Publication
Alpha-conotoxin Mr1.7a: acts as a weak partial agonist at alpha-7/CHRNA7 nicotinic acetylcholine receptors (nAChR) when tested in human SH-SY5Y neuroblastoma cells.1 Publication

Miscellaneous

Neither MrIC, Mr1.7b, nor Mr1.7a inhibit alpha-7/CHRNA7, alpha-3-beta-2/CHRNA3-CHRNB2 and alpha-3-beta-4/CHRNA3-CHRNB4 nAChRs endogenously expressed in human SH-SY5Y neuroblastoma cells. MrIC does not activate alpha-3-beta-2/CHRNA3-CHRNB2 and alpha-3-beta-4/CHRNA3-CHRNB4 endogenously expressed in human SH-SY5Y neuroblastoma cells. Mr1.7b does not activate alpha-7 endogenously expressed in human SH-SY5Y neuroblastoma cells (PubMed:24351107).1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin Mr1.7a
Alternative name(s):
Conotoxin Mr1.8
Cleaved into the following 2 chains:
Alternative name(s):
Mr1.7c
Alternative name(s):
Mr002
OrganismiConus marmoreus (Marble cone)
Taxonomic identifieri42752 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000043017422 – 49Add BLAST28
PeptideiPRO_000043017551 – 69Alpha-conotoxin Mr1.7bAdd BLAST19
PeptideiPRO_000043017652 – 68Alpha-conotoxin MrICAdd BLAST17
PeptideiPRO_000043017753 – 68Alpha-conotoxin Mr1.7aAdd BLAST16

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei524-hydroxyproline; in Mr1.7bCurated1
Disulfide bondi54 ↔ 60By similarity
Disulfide bondi55 ↔ 68By similarity
Modified residuei584-hydroxyproline; in Mr1.7bCurated1
Modified residuei68Cysteine amideBy similarity1

Post-translational modificationi

Two 4-hydroxyprolines have been detected by MS but the assignment of which of the three prolines is modified is uncertain (PubMed:23152539, and PubMed:24351107).

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha 4/7 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR009958. Conotoxin_a-typ.
PfamiView protein in Pfam
PF07365. Toxin_8. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F5C3U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMRMMFTVF LLVVLATTVV SFTSNRVLDP AFRRRNAAAK ASDLIALNAR
60
RPECCTHPAC HVSNPELCG
Length:69
Mass (Da):7,580
Last modified:June 28, 2011 - v1
Checksum:iB818681272597A55
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti69G → S in Mr1.7b. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF460791 mRNA. Translation: ADZ99331.1.

Similar proteinsi

Entry informationi

Entry nameiCA18_CONMR
AccessioniPrimary (citable) accession number: F5C3U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: June 28, 2011
Last modified: November 22, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

The cDNA sequence of this peptide has been submitted (EMBL entry JF460791) and described in PubMed:22781954 under the name Mr1.8.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families