Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi105 – 1051Magnesium; catalyticUniRule annotation
Sitei106 – 1061Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei132 – 1321Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei135 – 1351Proton acceptorUniRule annotation
Binding sitei237 – 2371SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciTSP499229:GHS2-484-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:TEPIRE1_0526Imported
OrganismiTepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)Imported
Taxonomic identifieri1209989 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeTepidanaerobacter
ProteomesiUP000010802 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1353Substrate bindingUniRule annotation
Regioni179 – 1813Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "B2" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiDETLLAM.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

F4LVA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNLVLAQS GGPTSVINAS CYGIIKEARK YEIKLYAAKF GIEGILQNNL
60 70 80 90 100
IDMTNISESD LNLLRYSPSA AFGSCRHKLK DEDFEKIIET FDKYQIGYFV
110 120 130 140 150
YIGGNDSMDT AHRLSEYIKK IGRDIKVIGV PKTIDNDLVN THFCPGYGSA
160 170 180 190 200
AKYIATSVRE MSLDADVYAK DIVTIVEVMG RNSGFLAAAG ALARDNVVSS
210 220 230 240 250
PHLIYFPEIP FDENEFLNDV KTTFKRNNGK VFIVASEGLK NKDGNYIYLN
260 270 280 290 300
EQKADAFGNV QMGGVGHYMR HLVTENITKR VKVVELSIIQ RCAAHAASLA
310 320 330 340 350
DNEAAVMVGE AAVKKIMDGV SDIMISIKNT DNGFEAGTVP LSEVANRTKL
360 370 380 390
LPSQWIDSKN RWVTQDAIDY MLPLIQGEVK VPYVNGIPKY IRFI
Length:394
Mass (Da):43,679
Last modified:June 28, 2011 - v1
Checksum:i4DE2794F288FC3B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF563609 Genomic DNA. Translation: CDI40405.1.
RefSeqiWP_013777599.1. NC_019954.2.
YP_004459983.1. NC_015519.1.

Genome annotation databases

EnsemblBacteriaiAEE90676; AEE90676; TepRe1_0478.
CDI40405; CDI40405; TepiRe1_0526.
KEGGitae:TepiRe1_0526.
tep:TepRe1_0478.
PATRICi54623850. VBITepSp18581_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF563609 Genomic DNA. Translation: CDI40405.1.
RefSeqiWP_013777599.1. NC_019954.2.
YP_004459983.1. NC_015519.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEE90676; AEE90676; TepRe1_0478.
CDI40405; CDI40405; TepiRe1_0526.
KEGGitae:TepiRe1_0526.
tep:TepRe1_0478.
PATRICi54623850. VBITepSp18581_0500.

Phylogenomic databases

KOiK00850.
OMAiDETLLAM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciTSP499229:GHS2-484-MONOMER.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "First genome sequence of a syntrophic acetate-oxidizing bacterium, Tepidanaerobacter acetatoxydans strain Re1."
    Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.
    Genome Announc. 1:E00213-E00213(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiF4LVA1_TEPAE
AccessioniPrimary (citable) accession number: F4LVA1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 27, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.