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F4LRZ6 (F4LRZ6_TEPAE) Unreviewed, UniProtKB/TrEMBL

Last modified March 19, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase HAMAP-Rule MF_00186

EC=2.7.1.30 HAMAP-Rule MF_00186
Alternative name(s):
ATP:glycerol 3-phosphotransferase HAMAP-Rule MF_00186
Glycerokinase HAMAP-Rule MF_00186
Gene names
Name:glpK HAMAP-Rule MF_00186 EMBL CDI40973.1
Ordered Locus Names:TepiRe1_2381 EMBL CDI40973.1, TepRe1_2213 EMBL AEE92335.1
OrganismTepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1) [Complete proteome] [HAMAP]
Taxonomic identifier1209989 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeTepidanaerobacter

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate By similarity. HAMAP-Rule MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP-Rule MF_00186 SAAS SAAS005999

Enzyme regulation

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP) By similarity. HAMAP-Rule MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP-Rule MF_00186 SAAS SAAS005999

Subunit structure

Homotetramer and homodimer (in equilibrium) By similarity. HAMAP-Rule MF_00186

Sequence similarities

Belongs to the FGGY kinase family. RuleBase RU003733 HAMAP-Rule MF_00186

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding11 – 133ATP By similarity HAMAP-Rule MF_00186
Nucleotide binding407 – 4115ATP By similarity HAMAP-Rule MF_00186
Region81 – 822Substrate binding By similarity HAMAP-Rule MF_00186
Region242 – 2432Substrate binding By similarity HAMAP-Rule MF_00186

Sites

Binding site111Substrate By similarity HAMAP-Rule MF_00186
Binding site151ATP By similarity HAMAP-Rule MF_00186
Binding site2641ATP By similarity HAMAP-Rule MF_00186
Binding site3061ATP; via carbonyl oxygen By similarity HAMAP-Rule MF_00186
Binding site3101ATP; via amide nitrogen By similarity HAMAP-Rule MF_00186
Binding site3251ATP By similarity HAMAP-Rule MF_00186

Sequences

Sequence LengthMass (Da)Tools
F4LRZ6 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 724C5367C8850590

FASTA49555,718
        10         20         30         40         50         60 
MGRILVIDQG TTGSRAIIFD EHGERLFMEY KEFRQIYPKP GWVEHDPMEI WDVTLDVTKR 

        70         80         90        100        110        120 
VLANANMSGK DISAIGITNQ RETTTLWDKN TGKPLYNSIV WACRRTTDIC QDLISKGYNE 

       130        140        150        160        170        180 
IFNKKTGLII DPVYSATKIR WIMDNVDGVK EKVDRDEVLF GTIDSWLLWN LTGGNVHATD 

       190        200        210        220        230        240 
FSNASRTMLF NVKDIKWDEE LLKIMDIPAN MLPEVKPSVG LFGYTDKKLF GKEIPITGIA 

       250        260        270        280        290        300 
GDQQAALFGQ TCFEEGDTKN TYGTSCVPLM FTGTKPVFTD KGLLTLAWGY NNEVKYSMGA 

       310        320        330        340        350        360 
SIFTAGSAIQ WLRDNLNLIS SSSETEELAK SVEDNAGVYF VPAFTGLGAP HWDMYARGMI 

       370        380        390        400        410        420 
IGLTSNATKA HLVRATLESL AYQTRDLLDE IESKSAIKLK CLKVDGGAAK NNFLMQFQAD 

       430        440        450        460        470        480 
ILNCPVIRPK DIETTSLGAA YMAGLGIGIW KDENEIKQLW KIDKEFYPSM QKDKREELYS 

       490 
NWKHAVEHAR GWLKK 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Tepidanaerobacter sp. Re1."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Pagani I., Teshima H., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Lcollab F.I., Woyke T.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 21804 / JCM 16047 / Re1 and Re1 EMBL AEE92335.1.
[2]"First Genome Sequence of a Syntrophic Acetate-Oxidizing Bacterium, Tepidanaerobacter acetatoxydans Strain Re1."
Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.
Genome Announc. 1:e00213-e00212(2013)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Re1 EMBL CDI40973.1.
[3]"First genome sequence of a syntrophic acetate-oxidizing bacterium, Tepidanaerobacter acetatoxydans strain Re1."
Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.
Genome Announc. 1:E00213-E00213(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002728 Genomic DNA. Translation: AEE92335.1.
HF563609 Genomic DNA. Translation: CDI40973.1.
RefSeqYP_004461642.1. NC_015519.1.
YP_008619215.1. NC_019954.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEE92335; AEE92335; TepRe1_2213.
GeneID10599002.
17374057.
KEGGtep:TepRe1_2213.
PATRIC54627585. VBITepSp18581_2319.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00864.

Enzyme and pathway databases

BioCycTSP499229:GHS2-2265-MONOMER.
UniPathwayUPA00618; UER00672.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
InterProIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4LRZ6_TEPAE
AccessionPrimary (citable) accession number: F4LRZ6
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: March 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)