Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol kinase

Gene

glpK

Organism
Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotationSAAS annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111SubstrateUniRule annotation
Binding sitei15 – 151ATPUniRule annotation
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei306 – 3061ATP; via carbonyl oxygenUniRule annotation
Binding sitei310 – 3101ATP; via amide nitrogenUniRule annotation
Binding sitei325 – 3251ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 133ATPUniRule annotation
Nucleotide bindingi407 – 4115ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glycerol kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: UniProtKB-HAMAP
  2. glycerol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

Glycerol metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSP499229:GHS2-2265-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotationSAAS annotation (EC:2.7.1.30UniRule annotationSAAS annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Gene namesi
Name:glpKUniRule annotationImported
Ordered Locus Names:TepiRe1_2381Imported, TepRe1_2213Imported
OrganismiTepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)Imported
Taxonomic identifieri1209989 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeTepidanaerobacter
ProteomesiUP000008463: Chromosome, UP000010802: Chromosome

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF4LRZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 822Substrate bindingUniRule annotation
Regioni242 – 2432Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

KOiK00864.
OMAiKYSMGAS.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4LRZ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRILVIDQG TTGSRAIIFD EHGERLFMEY KEFRQIYPKP GWVEHDPMEI
60 70 80 90 100
WDVTLDVTKR VLANANMSGK DISAIGITNQ RETTTLWDKN TGKPLYNSIV
110 120 130 140 150
WACRRTTDIC QDLISKGYNE IFNKKTGLII DPVYSATKIR WIMDNVDGVK
160 170 180 190 200
EKVDRDEVLF GTIDSWLLWN LTGGNVHATD FSNASRTMLF NVKDIKWDEE
210 220 230 240 250
LLKIMDIPAN MLPEVKPSVG LFGYTDKKLF GKEIPITGIA GDQQAALFGQ
260 270 280 290 300
TCFEEGDTKN TYGTSCVPLM FTGTKPVFTD KGLLTLAWGY NNEVKYSMGA
310 320 330 340 350
SIFTAGSAIQ WLRDNLNLIS SSSETEELAK SVEDNAGVYF VPAFTGLGAP
360 370 380 390 400
HWDMYARGMI IGLTSNATKA HLVRATLESL AYQTRDLLDE IESKSAIKLK
410 420 430 440 450
CLKVDGGAAK NNFLMQFQAD ILNCPVIRPK DIETTSLGAA YMAGLGIGIW
460 470 480 490
KDENEIKQLW KIDKEFYPSM QKDKREELYS NWKHAVEHAR GWLKK
Length:495
Mass (Da):55,718
Last modified:June 28, 2011 - v1
Checksum:i724C5367C8850590
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002728 Genomic DNA. Translation: AEE92335.1.
HF563609 Genomic DNA. Translation: CDI40973.1.
RefSeqiYP_004461642.1. NC_015519.1.
YP_008619215.1. NC_019954.2.

Genome annotation databases

EnsemblBacteriaiAEE92335; AEE92335; TepRe1_2213.
CDI40973; CDI40973; TepiRe1_2381.
GeneIDi10599002.
17374057.
KEGGitae:TepiRe1_2381.
tep:TepRe1_2213.
PATRICi54627585. VBITepSp18581_2319.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002728 Genomic DNA. Translation: AEE92335.1.
HF563609 Genomic DNA. Translation: CDI40973.1.
RefSeqiYP_004461642.1. NC_015519.1.
YP_008619215.1. NC_019954.2.

3D structure databases

ProteinModelPortaliF4LRZ6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEE92335; AEE92335; TepRe1_2213.
CDI40973; CDI40973; TepiRe1_2381.
GeneIDi10599002.
17374057.
KEGGitae:TepiRe1_2381.
tep:TepRe1_2213.
PATRICi54627585. VBITepSp18581_2319.

Phylogenomic databases

KOiK00864.
OMAiKYSMGAS.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciTSP499229:GHS2-2265-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Tepidanaerobacter sp. Re1."
    US DOE Joint Genome Institute
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Pagani I., Teshima H., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Lcollab F.I., Woyke T.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 21804 / JCM 16047 / Re1Imported and Re1Imported.
  2. "First Genome Sequence of a Syntrophic Acetate-Oxidizing Bacterium, Tepidanaerobacter acetatoxydans Strain Re1."
    Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.
    Genome Announc. 1:e00213-e00212(2013)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Re1Imported.
  3. "First genome sequence of a syntrophic acetate-oxidizing bacterium, Tepidanaerobacter acetatoxydans strain Re1."
    Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.
    Genome Announc. 1:E00213-E00213(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiF4LRZ6_TEPAE
AccessioniPrimary (citable) accession number: F4LRZ6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 4, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.