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Protein

Bifunctional cystathionine gamma-lyase/cysteine synthase

Gene

DES1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintaining Cys homeostasis through the desulfuration of L-cysteine. Modulates the generation of the signaling molecule sulfide in plant cytosol. Probably unable to interact with SAT and to form the decameric Cys synthase complex (CSC) and is therefore not an enzymatically true OASTL protein.3 Publications

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.1 Publication
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Inhibited by aminooxyacetate.1 Publication

Kineticsi

  1. KM=0.4 mM for L-cysteine for the cystathionine gamma-lyase activity1 Publication
  2. KM=5.2 mM for O(3)-acetyl-L-serine for the cysteine synthase activity1 Publication
  1. Vmax=0.04 µmol/min/mg enzyme for the DES reaction1 Publication
  2. Vmax=1.8 µmol/min/mg enzyme for the OASTL reaction1 Publication

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
  2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Pyridoxal phosphateBy similarity
Binding sitei271 – 2711Pyridoxal phosphateBy similarity

GO - Molecular functioni

  • cystathionine gamma-lyase activity Source: UniProtKB-EC
  • cysteine synthase activity Source: UniProtKB
  • L-cysteine desulfhydrase activity Source: UniProtKB
  • L-cystine L-cysteine-lyase (deaminating) Source: UniProtKB-EC
  • pyridoxal phosphate binding Source: UniProtKB
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • cysteine biosynthetic process from serine Source: GO_Central
  • cysteine homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional cystathionine gamma-lyase/cysteine synthase (EC:2.5.1.471 Publication, EC:4.4.1.11 Publication)
Alternative name(s):
Beta-substituted Ala synthase 4;3
Short name:
ARAth-Bsas4;3
L-cysteine desulfhydrase 1
Short name:
DES1
O-acetylserine (thiol)-lyase
Short name:
OASTL
Protein CS-LIKE
Gene namesi
Name:DES1
Ordered Locus Names:At5g28030
ORF Names:F15F15.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G28030.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Light early-flowering and premature leaf senescence phenotype. Increased total cysteine content and increased resistance to pathogens.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Bifunctional cystathionine gamma-lyase/cysteine synthasePRO_0000418598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiF4K5T2.
PRIDEiF4K5T2.

Expressioni

Gene expression databases

GenevisibleiF4K5T2. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G28030.1.

Structurei

3D structure databases

ProteinModelPortaliF4K5T2.
SMRiF4K5T2. Positions 7-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni183 – 1875Pyridoxal phosphate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi274 – 2796Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
InParanoidiF4K5T2.
KOiK01738.
OMAiGGKHGPH.
PhylomeDBiF4K5T2.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4K5T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRVLIKND VTELIGNTPM VYLNKIVDGC VARIAAKLEM MEPCSSIKDR
60 70 80 90 100
IAYSMIKDAE DKGLITPGKS TLIEATGGNT GIGLASIGAS RGYKVILLMP
110 120 130 140 150
STMSLERRII LRALGAEVHL TDISIGIKGQ LEKAKEILSK TPGGYIPHQF
160 170 180 190 200
INPENPEIHY RTTGPEIWRD SAGKVDILVA GVGTGGTVTG TGKFLKEKNK
210 220 230 240 250
DIKVCVVEPS ESAVLSGGKP GPHLIQGIGS GEIPANLDLS IVDEIIQVTG
260 270 280 290 300
EEAIETTKLL AIKEGLLVGI SSGASAAAAL KVAKRPENVG KLIVVIFPSG
310 320
GERYLSTELF ESVRYEAENL PVE
Length:323
Mass (Da):34,326
Last modified:June 28, 2011 - v1
Checksum:i5F8E4924D7CDED9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007627 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93765.1.
CP002688 Genomic DNA. Translation: AED93766.1.
RefSeqiNP_198155.1. NM_122686.1.
NP_974843.1. NM_203114.1.
UniGeneiAt.30769.

Genome annotation databases

EnsemblPlantsiAT5G28030.1; AT5G28030.1; AT5G28030.
AT5G28030.2; AT5G28030.2; AT5G28030.
GeneIDi832873.
GrameneiAT5G28030.1; AT5G28030.1; AT5G28030.
AT5G28030.2; AT5G28030.2; AT5G28030.
KEGGiath:AT5G28030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007627 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93765.1.
CP002688 Genomic DNA. Translation: AED93766.1.
RefSeqiNP_198155.1. NM_122686.1.
NP_974843.1. NM_203114.1.
UniGeneiAt.30769.

3D structure databases

ProteinModelPortaliF4K5T2.
SMRiF4K5T2. Positions 7-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G28030.1.

Proteomic databases

PaxDbiF4K5T2.
PRIDEiF4K5T2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G28030.1; AT5G28030.1; AT5G28030.
AT5G28030.2; AT5G28030.2; AT5G28030.
GeneIDi832873.
GrameneiAT5G28030.1; AT5G28030.1; AT5G28030.
AT5G28030.2; AT5G28030.2; AT5G28030.
KEGGiath:AT5G28030.

Organism-specific databases

TAIRiAT5G28030.

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
InParanoidiF4K5T2.
KOiK01738.
OMAiGGKHGPH.
PhylomeDBiF4K5T2.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.

Miscellaneous databases

PROiF4K5T2.

Gene expression databases

GenevisibleiF4K5T2. AT.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "beta-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis."
    Hatzfeld Y., Maruyama A., Schmidt A., Noji M., Ishizawa K., Saito K.
    Plant Physiol. 123:1163-1171(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  4. "Synthesis of the sulfur amino acids: cysteine and methionine."
    Wirtz M., Droux M.
    Photosyn. Res. 86:345-362(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis."
    Heeg C., Kruse C., Jost R., Gutensohn M., Ruppert T., Wirtz M., Hell R.
    Plant Cell 20:168-185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Physiological roles of the beta-substituted alanine synthase gene family in Arabidopsis."
    Watanabe M., Kusano M., Oikawa A., Fukushima A., Noji M., Saito K.
    Plant Physiol. 146:310-320(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, DISRUPTION PHENOTYPE.
  7. "An O-acetylserine(thiol)lyase homolog with L-cysteine desulfhydrase activity regulates cysteine homeostasis in Arabidopsis."
    Alvarez C., Calo L., Romero L.C., Garcia I., Gotor C.
    Plant Physiol. 152:656-669(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DISRUPTION PHENOTYPE.
  8. "Cysteine homeostasis plays an essential role in plant immunity."
    Alvarez C., Bermudez M.A., Romero L.C., Gotor C., Garcia I.
    New Phytol. 193:165-177(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  9. "L-Cysteine Desulfhydrase 1 modulates the generation of the signaling molecule sulfide in plant cytosol."
    Romero L.C., Garcia I., Gotor C.
    Plant Signal. Behav. 8:0-0(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCGL_ARATH
AccessioniPrimary (citable) accession number: F4K5T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 28, 2011
Last modified: June 8, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.