F4K1B1 (RPAP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog
EC=3.1.3.16

Alternative name(s):
RNA polymerase II-associated protein 2 homolog

Gene names

Ordered Locus Names:	At5g26760
ORF Names:	F2P16.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase involved in RNA polymerase II transcription regulation By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RPAP2 family. Contains 1 RTR1-type zinc finger.
Sequence caution	The sequence AAB61054.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: F4K1B1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: F4K1B1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-305: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog		PRO_0000416291
Regions
	Zinc finger	33 – 118	86	RTR1-type
Natural variations
	Alternative sequence	1 – 305	305	Missing in isoform 2.		VSP_042606

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: E782F1B107D5D5B7	FASTA	735	81,058
	10 20 30 40 50 60 MAKDNEAIAI NDAVHKLQLY MLENTTDQNQ LFAARKLMSR SDYEDVVTER AIAKLCGYTL 70 80 90 100 110 120 CQRFLPSDVS RRGKYRISLK DHKVYDLQET SKFCSAGCLI DSKTFSGSLQ EARTLEFDSV 130 140 150 160 170 180 KLNEILDLFG DSLEVKGSLD VNKDLDLSKL MIKENFGVRG EELSLEKWMG PSNAVEGYVP 190 200 210 220 230 240 FDRSKSSNDS KATTQSNQEK HEMDFTSTVI MPDVNSVSKL PPQTKQASTV VESVDGKGKT 250 260 270 280 290 300 VLKEQTVVPP TKKVSRFRRE KEKEKKTFGV DGMGCAQEKT TVLPRKILSF CNEIEKDFKN 310 320 330 340 350 360 FGFDEMGLAS SAMMSDGYGV EYSVSKQPQC SMEDSLSCKL KGDLQTLDGK NTLSGSSSGS 370 380 390 400 410 420 NTKGSKTKPE KSRKKIISVE YHANSYEDGE EILAAESYER HKAQDVCSSS EIVTKSCLKI 430 440 450 460 470 480 SGSKKLSRSV TWADQNDGRG DLCEVRNNDN AAGPSLSSND IEDVNSLSRL ALAEALATAL 490 500 510 520 530 540 SQAAEAVSSG NSDASDATAK AGIILLPSTH QLDEEVTEEH SEEEMTEEEP TLLKWPNKPG 550 560 570 580 590 600 IPDSDLFDRD QSWFDGPPEG FNLTLSNFAV MWDSLFGWVS SSSLAYIYGK EESAHEEFLL 610 620 630 640 650 660 VNGKEYPRRI IMVDGLSSEI KQTIAGCLAR ALPRVVTHLR LPIAISELEK GLGSLLETMS 670 680 690 700 710 720 LTGAVPSFRV KEWLVIVLLF LDALSVSRIP RIAPYISNRD KILEGSGIGN EEYETMKDIL 730 LPLGRVPQFA TRSGA
	Isoform 2 [UniParc]. Checksum: F409A9A8952D29BD	FASTA	430	46,685
	10 20 30 40 50 60 MGLASSAMMS DGYGVEYSVS KQPQCSMEDS LSCKLKGDLQ TLDGKNTLSG SSSGSNTKGS 70 80 90 100 110 120 KTKPEKSRKK IISVEYHANS YEDGEEILAA ESYERHKAQD VCSSSEIVTK SCLKISGSKK 130 140 150 160 170 180 LSRSVTWADQ NDGRGDLCEV RNNDNAAGPS LSSNDIEDVN SLSRLALAEA LATALSQAAE 190 200 210 220 230 240 AVSSGNSDAS DATAKAGIIL LPSTHQLDEE VTEEHSEEEM TEEEPTLLKW PNKPGIPDSD 250 260 270 280 290 300 LFDRDQSWFD GPPEGFNLTL SNFAVMWDSL FGWVSSSSLA YIYGKEESAH EEFLLVNGKE 310 320 330 340 350 360 YPRRIIMVDG LSSEIKQTIA GCLARALPRV VTHLRLPIAI SELEKGLGSL LETMSLTGAV 370 380 390 400 410 420 PSFRVKEWLV IVLLFLDALS VSRIPRIAPY ISNRDKILEG SGIGNEEYET MKDILLPLGR 430 VPQFATRSGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007270 Genomic DNA. Translation: AAB61054.1. Sequence problems. CP002688 Genomic DNA. Translation: AED93597.1. CP002688 Genomic DNA. Translation: AED93598.1. BT015786 mRNA. Translation: AAU90076.1.
IPI	IPI00526628. IPI00528441. IPI00547093.
PIR	T01757.
RefSeq	NP_198028.2. NM_122558.3. NP_974839.1. NM_203110.1.
UniGene	At.44801.
3D structure databases
ProteinModelPortal	F4K1B1.
ModBase	Search...
Proteomic databases
PRIDE	F4K1B1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G26760.2; AT5G26760.2; AT5G26760.
GeneID	832734.
KEGG	ath:AT5G26760.
Organism-specific databases
TAIR	At5g26760.
Phylogenomic databases
HOGENOM	HOG000241200.
OMA	VNGREYP.
PhylomeDB	F4K1B1.
Gene expression databases
ArrayExpress	F4K1B1.
Family and domain databases
InterPro	IPR007308. DUF408. [Graphical view]
Pfam	PF04181. RPAP2_Rtr1. 1 hit. [Graphical view]
PROSITE	PS51479. ZF_RTR1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPAP2_ARATH

Accession

Primary (citable) accession number: F4K1B1
Secondary accession number(s): O04626, Q5XF30

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2012
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program