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Protein

Membrane-bound transcription factor site-2 protease homolog

Gene

S2P

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metalloprotease that catalyzes the second step (site-2 cleavage) in the proteolytic activation of various factors, after site-1 cleavage. Part of a regulated intramembrane proteolysis (RIP) cascade. After ER stress, cleaves BZIP17 and BZIP28 proteins which functions as stress sensors and transducers in ER stress signaling pathway. The N-terminal bZIP component is translocated to the nucleus, where it activates the expression and production of ER chaperones, as well as proteins involved in brassinosteroid (BR) signaling, which is required for stress acclimation and growth.1 Publication

Cofactori

Zn2+Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251Zinc; catalyticPROSITE-ProRule annotation
Active sitei126 – 1261PROSITE-ProRule annotation
Metal bindingi129 – 1291Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: UniProtKB

GO - Biological processi

  • cellular hyperosmotic salinity response Source: UniProtKB
  • membrane protein intracellular domain proteolysis Source: GO_Central
  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM50.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound transcription factor site-2 protease homolog (EC:3.4.24.-)
Alternative name(s):
Endopeptidase S2P
Gene namesi
Name:S2P
Ordered Locus Names:At4g20310Imported
ORF Names:F1C12.220Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G20310.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060CytoplasmicCuratedAdd
BLAST
Transmembranei61 – 8121Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini82 – 10726LumenalCuratedAdd
BLAST
Transmembranei108 – 12821Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini129 – 1379CytoplasmicCurated
Transmembranei138 – 15821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini159 – 18224LumenalCuratedAdd
BLAST
Transmembranei183 – 20321Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini204 – 437234CytoplasmicCuratedAdd
BLAST
Transmembranei438 – 45821Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini459 – 48527LumenalCuratedAdd
BLAST
Transmembranei486 – 50621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini507 – 5137CytoplasmicCurated

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • Golgi membrane Source: UniProtKB
  • integral component of membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Short root and increased root branching. Mutant plants have increased sensitivity to salt-induced osmotic stress and tunicamycin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Membrane-bound transcription factor site-2 protease homologPRO_0000431970Add
BLAST

Proteomic databases

PaxDbiF4JUU5.
PRIDEiF4JUU5.

Expressioni

Tissue specificityi

Expressed in the vasculature of roots, cotyledons and leaves.1 Publication

Gene expression databases

ExpressionAtlasiF4JUU5. baseline and differential.
GenevisibleiF4JUU5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G20310.2.

Structurei

3D structure databases

ProteinModelPortaliF4JUU5.
SMRiF4JUU5. Positions 213-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 1712Arg-richPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M50A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2921. Eukaryota.
COG0750. LUCA.
HOGENOMiHOG000070842.
InParanoidiF4JUU5.
KOiK07765.
OrthoDBiEOG09360J8Z.

Family and domain databases

InterProiIPR001193. MBTPS2.
IPR008915. Peptidase_M50.
[Graphical view]
PANTHERiPTHR13325. PTHR13325. 1 hit.
PfamiPF02163. Peptidase_M50. 1 hit.
[Graphical view]
PRINTSiPR01000. SREBPS2PTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.Curated
Isoform 1 (identifier: F4JUU5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEISGRRMRR FRMRFRRDHL TGGENIENEA SCCYCDLKIS NFNEPIFRLG
60 70 80 90 100
RRFSGVLKVW FSIGLGFGVA SLILVTVFLL LQFHSNPLFS NRLTSAVFGF
110 120 130 140 150
SPSTRVSLSG IAYVLVSTVI TVSVHELGHA LAAASEGIQM EYIAVFIAAI
160 170 180 190 200
FPGGLVAFDN DVLQSLPSFN ALRIYCAGIW HNAVFCALCV FALFLLPVML
210 220 230 240 250
SPFYKHGESL TVVDVPSVSP LFGYLSPGDV IVSLDGIQVH KPSEWLELAA
260 270 280 290 300
ILDKENSKTS NGSLYLGGSR RFHHGKGYCV PISLIEEGYK GKMVENQFVC
310 320 330 340 350
PGDLTAFRTM PCSNAAIREV SVCLDAKDIV KLQKCGDGWV TTSDTDNQSD
360 370 380 390 400
CVCPQGDLCL QAMQSPGVLW TEITYKRTSS QDCSRLGLDF NTSNCLGTFV
410 420 430 440 450
FVGDLIAMSH SVHLTAYQPR WLFNFFGKSF PNILERSLTC TFHVSLALVL
460 470 480 490 500
LNSLPVYYLD GESILESSLQ SFTWLSPRKK KKALQVCLVG GSLLSFLAFF
510
RIFLLGLPLS RRW
Length:513
Mass (Da):56,824
Last modified:March 4, 2015 - v2
Checksum:i6544814A3813D122
GO

Sequence cautioni

The sequence AEE84303 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA18255 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79031 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022224 Genomic DNA. Translation: CAA18255.1. Sequence problems.
AL161552 Genomic DNA. Translation: CAB79031.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84303.1. Sequence problems.
PIRiT05339.
RefSeqiNP_001154255.1. NM_001160783.1.
UniGeneiAt.45338.

Genome annotation databases

EnsemblPlantsiAT4G20310.2; AT4G20310.2; AT4G20310.
GeneIDi827778.
GrameneiAT4G20310.2; AT4G20310.2; AT4G20310.
KEGGiath:AT4G20310.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022224 Genomic DNA. Translation: CAA18255.1. Sequence problems.
AL161552 Genomic DNA. Translation: CAB79031.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84303.1. Sequence problems.
PIRiT05339.
RefSeqiNP_001154255.1. NM_001160783.1.
UniGeneiAt.45338.

3D structure databases

ProteinModelPortaliF4JUU5.
SMRiF4JUU5. Positions 213-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G20310.2.

Protein family/group databases

MEROPSiM50.009.

Proteomic databases

PaxDbiF4JUU5.
PRIDEiF4JUU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G20310.2; AT4G20310.2; AT4G20310.
GeneIDi827778.
GrameneiAT4G20310.2; AT4G20310.2; AT4G20310.
KEGGiath:AT4G20310.

Organism-specific databases

TAIRiAT4G20310.

Phylogenomic databases

eggNOGiKOG2921. Eukaryota.
COG0750. LUCA.
HOGENOMiHOG000070842.
InParanoidiF4JUU5.
KOiK07765.
OrthoDBiEOG09360J8Z.

Miscellaneous databases

PROiF4JUU5.

Gene expression databases

ExpressionAtlasiF4JUU5. baseline and differential.
GenevisibleiF4JUU5. AT.

Family and domain databases

InterProiIPR001193. MBTPS2.
IPR008915. Peptidase_M50.
[Graphical view]
PANTHERiPTHR13325. PTHR13325. 1 hit.
PfamiPF02163. Peptidase_M50. 1 hit.
[Graphical view]
PRINTSiPR01000. SREBPS2PTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS2P_ARATH
AccessioniPrimary (citable) accession number: F4JUU5
Secondary accession number(s): O65444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: March 4, 2015
Last modified: September 7, 2016
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.