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F4JSH1

- APY7_ARATH

UniProt

F4JSH1 - APY7_ARATH

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Protein

Probable apyrase 7

Gene

APY7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity. Involved in the regulation of pollen and anther development.By similarity1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei284 – 2841Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi147 – 15711ATP-bindingCuratedAdd
BLAST
Nucleotide bindingi309 – 31911ATP-bindingCuratedAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. anther dehiscence Source: TAIR
  2. pollen exine formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 7 (EC:3.6.1.5)
Short name:
AtAPY7
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 7
Gene namesi
Name:APY7
Ordered Locus Names:At4g19180
ORF Names:T18B16.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G19180.

Subcellular locationi

Membrane By similarity; Multi-pass membrane protein By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Apy6 and dapy7 double mutant exhibits late anther dehiscence and low male fertility. Pollen grains of double mutant are largely deformed in shape and in most cases, the cell walls of the pollen grains are interconnected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Probable apyrase 7PRO_0000420345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiF4JSH1.

Expressioni

Tissue specificityi

Detected in mature pollen grains. Also expressed in more diverse tissues such as roots, leaves, stems, pistils and sepals. More particularly expressed in the vascular bundle.1 Publication

Inductioni

By wounding and drought stress.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G19180.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4JSH1.
SMRiF4JSH1. Positions 144-559.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 113113CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini135 – 581447ExtracellularSequence AnalysisAdd
BLAST
Topological domaini603 – 740138CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei114 – 13421HelicalSequence AnalysisAdd
BLAST
Transmembranei582 – 60221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi625 – 735111Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
InParanoidiF4JSH1.
OMAiSKLVHNE.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4JSH1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVFGRITELF TAASSRLPAG SQSSVPYMPT GSSPDVGTSV SDSISIGNGG
60 70 80 90 100
RKNCLRHSAS LQDFSSYHGF DPEESILPRE AISWGQNGSS FSKEKGSVPN
110 120 130 140 150
GTNPSTRRKL IRAVMIVMCL FLFAFLVYIV SMYIYTNWSR GASRYYVVFD
160 170 180 190 200
CGSTGTRAYV YQASINYKKD SSLPIVMKSL TEGISRKSRG RAYDRMETEP
210 220 230 240 250
GFDKLVNNRT GLKTAIKPLI QWAEKQIPKN AHRTTSLFVY ATAGVRRLRP
260 270 280 290 300
ADSSWILGNV WSILAKSPFT CRREWVKIIS GTEEAYFGWT ALNYQTSMLG
310 320 330 340 350
ALPKKATFGA LDLGGSSLQV TFENEERTHN ETNLNLRIGS VNHHLSAYSL
360 370 380 390 400
AGYGLNDAFD RSVVHLLKKL PNVNKSDLIE GKLEMKHPCL NSGYNGQYIC
410 420 430 440 450
SQCASSVQGG KKGKSGVSIK LVGAPNWGEC SALAKNAVNS SEWSNAKHGV
460 470 480 490 500
DCDLQPCALP DGYPRPHGQF YAVSGFFVVY RFFNLSAEAS LDDVLEKGRE
510 520 530 540 550
FCDKAWQVAR TSVSPQPFIE QYCFRAPYIV SLLREGLYIT DKQIIIGSGS
560 570 580 590 600
ITWTLGVALL ESGKALSSTL GLKSYETLSM KINPIALISI LILSLLLLLC
610 620 630 640 650
ALSRVSNCLP RFFRKSYLPL FRHNSTSASS VLNIPSPFRF QRWSPMSTGV
660 670 680 690 700
KTPLSPTVRG SPRRPFSFGS SIQLMESSLY SSSSCVMHSC SSDSLGDIQY
710 720 730 740
DSTGSFWSSP RRSQMRLQSR RSQSREDLSS SLADSHMLKM
Length:740
Mass (Da):81,683
Last modified:June 28, 2011 - v1
Checksum:i4B282DAFC67F4ED0
GO

Sequence cautioni

The sequence CAA16707.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At4g19180 and At4g19185.
The sequence CAB78920.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At4g19180 and At4g19185.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830012 mRNA. Translation: AEJ38088.1.
JQ965809 mRNA. Translation: AFL69929.1.
AL021687 Genomic DNA. Translation: CAA16707.1. Sequence problems.
AL161550 Genomic DNA. Translation: CAB78920.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84155.1.
PIRiT04439.
RefSeqiNP_567579.2. NM_118037.6.
UniGeneiAt.32833.

Genome annotation databases

EnsemblPlantsiAT4G19180.1; AT4G19180.1; AT4G19180.
GeneIDi827656.
KEGGiath:AT4G19180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830012 mRNA. Translation: AEJ38088.1 .
JQ965809 mRNA. Translation: AFL69929.1 .
AL021687 Genomic DNA. Translation: CAA16707.1 . Sequence problems.
AL161550 Genomic DNA. Translation: CAB78920.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84155.1 .
PIRi T04439.
RefSeqi NP_567579.2. NM_118037.6.
UniGenei At.32833.

3D structure databases

ProteinModelPortali F4JSH1.
SMRi F4JSH1. Positions 144-559.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G19180.1-P.

Proteomic databases

PRIDEi F4JSH1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G19180.1 ; AT4G19180.1 ; AT4G19180 .
GeneIDi 827656.
KEGGi ath:AT4G19180.

Organism-specific databases

TAIRi AT4G19180.

Phylogenomic databases

eggNOGi COG5371.
InParanoidi F4JSH1.
OMAi SKLVHNE.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analyses of Arabidopsis apyrases 3 through 7."
    Yang J.
    Thesis (2011), University of Texas, United States
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION, FUNCTION.
    Strain: cv. Columbia.
  2. "Cloning the Arabidopsis apyrase gene, APY7."
    Lao J., Loque D., Heazlewood J.L.
    Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Stem.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiAPY7_ARATH
AccessioniPrimary (citable) accession number: F4JSH1
Secondary accession number(s): O49676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3