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Protein

Replication protein A 70 kDa DNA-binding subunit E

Gene

RPA1E

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi241 – 327OBAdd BLAST87
Zinc fingeri532 – 558C4-typeSequence analysisAdd BLAST27

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein A 70 kDa DNA-binding subunit E
Short name:
AtRPA70E
Alternative name(s):
AtRPA1-1
Replication factor A protein 1E
Replication protein A 1E
Short name:
AtRPA1E
Gene namesi
Name:RPA1E
Synonyms:RPA70E
Ordered Locus Names:At4g19130
ORF Names:T18B16.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G19130.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004226191 – 784Replication protein A 70 kDa DNA-binding subunit EAdd BLAST784

Proteomic databases

PaxDbiF4JSG3.

Expressioni

Gene expression databases

GenevisibleiF4JSG3. AT.

Interactioni

Subunit structurei

Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex).By similarity

Protein-protein interaction databases

STRINGi3702.AT4G19130.1.

Structurei

3D structure databases

ProteinModelPortaliF4JSG3.
SMRiF4JSG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 OB DNA-binding domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri532 – 558C4-typeSequence analysisAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0851. Eukaryota.
COG1599. LUCA.
InParanoidiF4JSG3.
KOiK07466.
OMAiCDVEGQQ.
OrthoDBiEOG093601YN.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR013955. Rep_factor-A_C.
IPR007199. Rep_factor-A_N.
IPR031657. REPA_OB_2.
IPR004591. Rfa1.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF04057. Rep-A_N. 1 hit.
PF08646. Rep_fac-A_C. 1 hit.
PF16900. REPA_OB_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
TIGRFAMsiTIGR00617. rpa1. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4JSG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVSLTAGAI GKIMNGEVTT EADMIPVLQV TDLKQIMAQQ DPTRERFRMV
60 70 80 90 100
LSDGTYLHQG MLGTDLNNLV KEGTLQPGSI VRLTRFVGDV IKGRRIVIVP
110 120 130 140 150
QLEVLKQISD IIGHPVPGGK HNDQRGADSG IKFNTTEQQG SGIRQVNNIE
160 170 180 190 200
PGRSNAAISP QVGGTGSSVP ASTTPSTRAY SNPSSGNGVT RQDYARDPPT
210 220 230 240 250
SYPHQPQPPP PMYANRGPVA RNEAPPKIIP VNALSPYSGR WTIKARVTNK
260 270 280 290 300
AALKQYSNPR GEGKVFNFDL LDADGGEIRV TCFNAVADQF YDQIVVGNLY
310 320 330 340 350
LISRGSLRPA QKNFNHLRND YEIMLDNAST IKQCYEEDAA IPRHQFHFRT
360 370 380 390 400
IGDIESMENN CIVDVIGIVS SISPTVTITR KNGTATPKRS LQLKDMSGRS
410 420 430 440 450
VEVTMWGDFC NAEGQRLQSL CDSGVFPVLA VKAGRISEFN GKTVSTIGSS
460 470 480 490 500
QLFIDPDFVE AEKLKNWFER EGKSVPCISL SREFSGSGKV DVRKTISQIK
510 520 530 540 550
DEKLGTSEKP DWITVSATIL YLKFDNFCYT ACPIMNGDRP CSKKVTDNGD
560 570 580 590 600
GTWRCEKCDK SVDECDYRYI LQLQIQDHTD LTCVTAFQEA GEEIMGISAK
610 620 630 640 650
DLYYVKNEHK DEEKFEDIIR KVAFTKYNFK LKVKEETFSD EQRVKATVVK
660 670 680 690 700
VDKLNYSADT RTMLGAMDKL RTRDANSLPI NPEGSDYNAD VVNTGIGSSG
710 720 730 740 750
TRDPSSVQRR DFGLHAHQSG QSGNHYSGGG ATTSCNVCGN SGHVSAKCPG
760 770 780
ATKPQEQGQY MGGSYRGTTG SYGGGLPRQH VGSY
Length:784
Mass (Da):85,992
Last modified:June 28, 2011 - v1
Checksum:i507E62118F205CB8
GO

Sequence cautioni

The sequence CAA16702 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78915 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti643R → C in AF439837 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021687 Genomic DNA. Translation: CAA16702.1. Sequence problems.
AL161550 Genomic DNA. Translation: CAB78915.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84147.1.
AF439837 mRNA. No translation available.
PIRiT04434.
RefSeqiNP_567576.2. NM_118032.2.
UniGeneiAt.26624.
At.73929.

Genome annotation databases

EnsemblPlantsiAT4G19130.1; AT4G19130.1; AT4G19130.
GeneIDi827651.
GrameneiAT4G19130.1; AT4G19130.1; AT4G19130.
KEGGiath:AT4G19130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021687 Genomic DNA. Translation: CAA16702.1. Sequence problems.
AL161550 Genomic DNA. Translation: CAB78915.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84147.1.
AF439837 mRNA. No translation available.
PIRiT04434.
RefSeqiNP_567576.2. NM_118032.2.
UniGeneiAt.26624.
At.73929.

3D structure databases

ProteinModelPortaliF4JSG3.
SMRiF4JSG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G19130.1.

Proteomic databases

PaxDbiF4JSG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G19130.1; AT4G19130.1; AT4G19130.
GeneIDi827651.
GrameneiAT4G19130.1; AT4G19130.1; AT4G19130.
KEGGiath:AT4G19130.

Organism-specific databases

TAIRiAT4G19130.

Phylogenomic databases

eggNOGiKOG0851. Eukaryota.
COG1599. LUCA.
InParanoidiF4JSG3.
KOiK07466.
OMAiCDVEGQQ.
OrthoDBiEOG093601YN.

Miscellaneous databases

PROiF4JSG3.

Gene expression databases

GenevisibleiF4JSG3. AT.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR013955. Rep_factor-A_C.
IPR007199. Rep_factor-A_N.
IPR031657. REPA_OB_2.
IPR004591. Rfa1.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF04057. Rep-A_N. 1 hit.
PF08646. Rep_fac-A_C. 1 hit.
PF16900. REPA_OB_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
TIGRFAMsiTIGR00617. rpa1. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFA1E_ARATH
AccessioniPrimary (citable) accession number: F4JSG3
Secondary accession number(s): O49671
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: June 28, 2011
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.