Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diacylglycerol kinase 7

Gene

DGK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress xhibits high specificity for 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG).2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.74 mM for ATP1 Publication
  1. Vmax=0.34 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol1 Publication

pH dependencei

Optimum pH is 6.8.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: TAIR
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • leaf development Source: TAIR
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • root development Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processPlant defense, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT4G30340-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 7 (EC:2.7.1.107)
Short name:
AtDGK7
Short name:
DAG kinase 7
Alternative name(s):
Diglyceride kinase 7
Short name:
DGK 7
Gene namesi
Name:DGK7
Ordered Locus Names:At4g30340
ORF Names:F17I23.320
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30340
TAIRilocus:2118791 AT4G30340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221151 – 492Diacylglycerol kinase 7Add BLAST492

Proteomic databases

PaxDbiF4JQ95
PRIDEiF4JQ95

PTM databases

iPTMnetiF4JQ95

Expressioni

Tissue specificityi

Highly expressed in flowers, and at low levels in roots, stems and leaves.1 Publication

Gene expression databases

ExpressionAtlasiF4JQ95 baseline and differential
GenevisibleiF4JQ95 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi14444, 1 interactor
STRINGi3702.AT4G30340.1

Structurei

3D structure databases

ProteinModelPortaliF4JQ95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 248DAGKcPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
HOGENOMiHOG000239559
InParanoidiF4JQ95
KOiK00901
OMAiPEKASCY
OrthoDBiEOG09360AZ1

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016961 Diacylglycerol_kinase_pln
IPR016064 NAD/diacylglycerol_kinase_sf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PIRSFiPIRSF030829 Diacylglycerol_kinase_pln, 1 hit
SMARTiView protein in SMART
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequencei

Sequence statusi: Complete.

F4JQ95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETPRSVGE ASTTNFVAAR PSAKTDDAVT MRGCGFANLA LVGIDKEELR
60 70 80 90 100
GRLAMPEYLR IAMRDCIKRK DSTEISDHLL LPGGAAADMA PHAPMVVFIN
110 120 130 140 150
PKSGGRHGPV LKERLQQLMT EEQVFDLTEV KPHEFVRYGL GCLDTLAAKG
160 170 180 190 200
DECARECREK IRIMVAGGDG TVGWVLGCLG ELHKDGKSHI PPVGVIPLGT
210 220 230 240 250
GNDLSRSFSW GGSFPFAWRS AMKRTLHRAT LGSIARLDSW KIVVSMPSGE
260 270 280 290 300
VVDPPYSLKP TIEETALDQA LDADGDVPPK AKSYEGVFYN YFSIGMDAQV
310 320 330 340 350
AYGFHHLRNE KPYLAQGPVT NKIIYSSYSC TQGWFCTPCV NNPALRGLRN
360 370 380 390 400
IMKIHIKKAN CSEWEEIHVP KSVRSIVVLN LYNYGSGRHP WGNLRPKYLE
410 420 430 440 450
KRGFVEAHCD DGLIEIFGLK QGWHASFVMA EIISAKHIAQ AAAIRFELRG
460 470 480 490
GDWKNAFLQM DGEPWKQPMK SDYSTFVEIK KVPFQSLMIN GE
Length:492
Mass (Da):54,572
Last modified:June 28, 2011 - v1
Checksum:i7E5577CA2799D3A2
GO

Sequence cautioni

The sequence AAK25884 differs from that shown. Intron retention.Curated
The sequence AAM44963 differs from that shown. Intron retention.Curated
The sequence AAU04880 differs from that shown. Intron retention.Curated
The sequence CAB81027 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274D → G in ABC71078 (PubMed:16081412).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686593 mRNA Translation: AAU04880.1 Sequence problems.
DQ350135 mRNA Translation: ABC71078.1
AL161576 Genomic DNA Translation: CAB81027.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE85754.1
AF360174 mRNA Translation: AAK25884.1 Sequence problems.
AY113915 mRNA Translation: AAM44963.1 Sequence problems.
PIRiG85354
RefSeqiNP_001328552.1, NM_001342006.1
NP_567845.4, NM_119180.6
UniGeneiAt.4562

Genome annotation databases

EnsemblPlantsiAT4G30340.1; AT4G30340.1; AT4G30340
GeneIDi829157
GrameneiAT4G30340.1; AT4G30340.1; AT4G30340
KEGGiath:AT4G30340

Similar proteinsi

Entry informationi

Entry nameiDGK7_ARATH
AccessioniPrimary (citable) accession number: F4JQ95
Secondary accession number(s): B0KYU1, Q9C5L2, Q9M0C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health