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Protein

Diacylglycerol kinase 7

Gene

DGK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress xhibits high specificity for 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG).2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.74 mM for ATP1 Publication
  1. Vmax=0.34 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol1 Publication

pH dependencei

Optimum pH is 6.8.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: TAIR
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • leaf development Source: TAIR
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 7 (EC:2.7.1.107)
Short name:
AtDGK7
Short name:
DAG kinase 7
Alternative name(s):
Diglyceride kinase 7
Short name:
DGK 7
Gene namesi
Name:DGK7
Ordered Locus Names:At4g30340
ORF Names:F17I23.320
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30340.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Diacylglycerol kinase 7PRO_0000422115Add
BLAST

Proteomic databases

PRIDEiF4JQ95.

Expressioni

Tissue specificityi

Highly expressed in flowers, and at low levels in roots, stems and leaves.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G30340.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4JQ95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 248159DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000239559.
InParanoidiF4JQ95.
KOiK00901.
OMAiGRHPWGN.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTiSM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4JQ95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETPRSVGE ASTTNFVAAR PSAKTDDAVT MRGCGFANLA LVGIDKEELR
60 70 80 90 100
GRLAMPEYLR IAMRDCIKRK DSTEISDHLL LPGGAAADMA PHAPMVVFIN
110 120 130 140 150
PKSGGRHGPV LKERLQQLMT EEQVFDLTEV KPHEFVRYGL GCLDTLAAKG
160 170 180 190 200
DECARECREK IRIMVAGGDG TVGWVLGCLG ELHKDGKSHI PPVGVIPLGT
210 220 230 240 250
GNDLSRSFSW GGSFPFAWRS AMKRTLHRAT LGSIARLDSW KIVVSMPSGE
260 270 280 290 300
VVDPPYSLKP TIEETALDQA LDADGDVPPK AKSYEGVFYN YFSIGMDAQV
310 320 330 340 350
AYGFHHLRNE KPYLAQGPVT NKIIYSSYSC TQGWFCTPCV NNPALRGLRN
360 370 380 390 400
IMKIHIKKAN CSEWEEIHVP KSVRSIVVLN LYNYGSGRHP WGNLRPKYLE
410 420 430 440 450
KRGFVEAHCD DGLIEIFGLK QGWHASFVMA EIISAKHIAQ AAAIRFELRG
460 470 480 490
GDWKNAFLQM DGEPWKQPMK SDYSTFVEIK KVPFQSLMIN GE
Length:492
Mass (Da):54,572
Last modified:June 28, 2011 - v1
Checksum:i7E5577CA2799D3A2
GO

Sequence cautioni

The sequence AAK25884.1 differs from that shown.Intron retention.Curated
The sequence AAM44963.1 differs from that shown.Intron retention.Curated
The sequence AAU04880.1 differs from that shown.Intron retention.Curated
The sequence CAB81027.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741D → G in ABC71078 (PubMed:16081412).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686593 mRNA. Translation: AAU04880.1. Sequence problems.
DQ350135 mRNA. Translation: ABC71078.1.
AL161576 Genomic DNA. Translation: CAB81027.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85754.1.
AF360174 mRNA. Translation: AAK25884.1. Sequence problems.
AY113915 mRNA. Translation: AAM44963.1. Sequence problems.
PIRiG85354.
RefSeqiNP_567845.4. NM_119180.5.
UniGeneiAt.4562.

Genome annotation databases

EnsemblPlantsiAT4G30340.1; AT4G30340.1; AT4G30340.
GeneIDi829157.
KEGGiath:AT4G30340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY686593 mRNA. Translation: AAU04880.1. Sequence problems.
DQ350135 mRNA. Translation: ABC71078.1.
AL161576 Genomic DNA. Translation: CAB81027.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85754.1.
AF360174 mRNA. Translation: AAK25884.1. Sequence problems.
AY113915 mRNA. Translation: AAM44963.1. Sequence problems.
PIRiG85354.
RefSeqiNP_567845.4. NM_119180.5.
UniGeneiAt.4562.

3D structure databases

ProteinModelPortaliF4JQ95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G30340.1-P.

Proteomic databases

PRIDEiF4JQ95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30340.1; AT4G30340.1; AT4G30340.
GeneIDi829157.
KEGGiath:AT4G30340.

Organism-specific databases

TAIRiAT4G30340.

Phylogenomic databases

HOGENOMiHOG000239559.
InParanoidiF4JQ95.
KOiK00901.
OMAiGRHPWGN.

Miscellaneous databases

PROiF4JQ95.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTiSM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
    Gomez-Merino F.C., Arana-Ceballos F.A., Trejo-Tellez L.I., Skirycz A., Brearley C.A., Doermann P., Mueller-Roeber B.
    J. Biol. Chem. 280:34888-34899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "Arabidopsis AtDGK7 regulates lateral root formation."
    Arana-Ceballos F.A., Gomez-Merino F., Krebs J., Skirycz A., Mueller-Roeber B.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase."
    Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A., Munnik T.
    Front. Plant Sci. 4:1-1(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDGK7_ARATH
AccessioniPrimary (citable) accession number: F4JQ95
Secondary accession number(s): B0KYU1, Q9C5L2, Q9M0C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 28, 2011
Last modified: May 27, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.