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F4JQ95

- DGK7_ARATH

UniProt

F4JQ95 - DGK7_ARATH

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Protein

Diacylglycerol kinase 7

Gene
DGK7, At4g30340, F17I23.320
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress xhibits high specificity for 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG).2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.74 mM for ATP1 Publication

Vmax=0.34 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol

pH dependencei

Optimum pH is 6.8.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: TAIR
  3. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. defense response Source: UniProtKB-KW
  2. leaf development Source: TAIR
  3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  4. root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase 7 (EC:2.7.1.107)
Short name:
AtDGK7
Short name:
DAG kinase 7
Alternative name(s):
Diglyceride kinase 7
Short name:
DGK 7
Gene namesi
Name:DGK7
Ordered Locus Names:At4g30340
ORF Names:F17I23.320
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G30340.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Diacylglycerol kinase 7PRO_0000422115Add
BLAST

Proteomic databases

PRIDEiF4JQ95.

Expressioni

Tissue specificityi

Highly expressed in flowers, and at low levels in roots, stems and leaves.1 Publication

Gene expression databases

ArrayExpressiF4JQ95.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi3702.AT4G30340.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4JQ95.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 248159DAGKcAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Phylogenomic databases

KOiK00901.
OMAiDECAREC.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
[Graphical view]
PfamiPF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTiSM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4JQ95-1 [UniParc]FASTAAdd to Basket

« Hide

MEETPRSVGE ASTTNFVAAR PSAKTDDAVT MRGCGFANLA LVGIDKEELR    50
GRLAMPEYLR IAMRDCIKRK DSTEISDHLL LPGGAAADMA PHAPMVVFIN 100
PKSGGRHGPV LKERLQQLMT EEQVFDLTEV KPHEFVRYGL GCLDTLAAKG 150
DECARECREK IRIMVAGGDG TVGWVLGCLG ELHKDGKSHI PPVGVIPLGT 200
GNDLSRSFSW GGSFPFAWRS AMKRTLHRAT LGSIARLDSW KIVVSMPSGE 250
VVDPPYSLKP TIEETALDQA LDADGDVPPK AKSYEGVFYN YFSIGMDAQV 300
AYGFHHLRNE KPYLAQGPVT NKIIYSSYSC TQGWFCTPCV NNPALRGLRN 350
IMKIHIKKAN CSEWEEIHVP KSVRSIVVLN LYNYGSGRHP WGNLRPKYLE 400
KRGFVEAHCD DGLIEIFGLK QGWHASFVMA EIISAKHIAQ AAAIRFELRG 450
GDWKNAFLQM DGEPWKQPMK SDYSTFVEIK KVPFQSLMIN GE 492
Length:492
Mass (Da):54,572
Last modified:June 28, 2011 - v1
Checksum:i7E5577CA2799D3A2
GO

Sequence cautioni

The sequence AAK25884.1 differs from that shown. Reason: Intron retention.
The sequence AAM44963.1 differs from that shown. Reason: Intron retention.
The sequence AAU04880.1 differs from that shown. Reason: Intron retention.
The sequence CAB81027.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741D → G in ABC71078. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY686593 mRNA. Translation: AAU04880.1. Sequence problems.
DQ350135 mRNA. Translation: ABC71078.1.
AL161576 Genomic DNA. Translation: CAB81027.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85754.1.
AF360174 mRNA. Translation: AAK25884.1. Sequence problems.
AY113915 mRNA. Translation: AAM44963.1. Sequence problems.
PIRiG85354.
RefSeqiNP_567845.4. NM_119180.5.
UniGeneiAt.4562.

Genome annotation databases

EnsemblPlantsiAT4G30340.1; AT4G30340.1; AT4G30340.
GeneIDi829157.
KEGGiath:AT4G30340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY686593 mRNA. Translation: AAU04880.1 . Sequence problems.
DQ350135 mRNA. Translation: ABC71078.1 .
AL161576 Genomic DNA. Translation: CAB81027.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE85754.1 .
AF360174 mRNA. Translation: AAK25884.1 . Sequence problems.
AY113915 mRNA. Translation: AAM44963.1 . Sequence problems.
PIRi G85354.
RefSeqi NP_567845.4. NM_119180.5.
UniGenei At.4562.

3D structure databases

ProteinModelPortali F4JQ95.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G30340.1-P.

Proteomic databases

PRIDEi F4JQ95.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G30340.1 ; AT4G30340.1 ; AT4G30340 .
GeneIDi 829157.
KEGGi ath:AT4G30340.

Organism-specific databases

TAIRi AT4G30340.

Phylogenomic databases

KOi K00901.
OMAi DECAREC.

Gene expression databases

ArrayExpressi F4JQ95.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016961. Diacylglycerol_kinase_pln.
[Graphical view ]
Pfami PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
PIRSFi PIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
SMARTi SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
    Gomez-Merino F.C., Arana-Ceballos F.A., Trejo-Tellez L.I., Skirycz A., Brearley C.A., Doermann P., Mueller-Roeber B.
    J. Biol. Chem. 280:34888-34899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  2. "Arabidopsis AtDGK7 regulates lateral root formation."
    Arana-Ceballos F.A., Gomez-Merino F., Krebs J., Skirycz A., Mueller-Roeber B.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase."
    Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A., Munnik T.
    Front. Plant Sci. 4:1-1(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDGK7_ARATH
AccessioniPrimary (citable) accession number: F4JQ95
Secondary accession number(s): B0KYU1, Q9C5L2, Q9M0C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi