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F4JQ95

- DGK7_ARATH

UniProt

F4JQ95 - DGK7_ARATH

Protein

Diacylglycerol kinase 7

Gene

DGK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress xhibits high specificity for 1,2-dioleoyl-sn-glycerol (1,2-DOG), 1-palmitoyl, 2-oleoyl-sn-glycerol (1,2 POG), 1-stearoyl, 2-linoleoyl-sn-glycerol (1,2-SLG) and 1-oleoyl, 2-palmitoyl-sn-glycerol (1,2-OPG).2 Publications

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

    Kineticsi

    1. KM=0.74 mM for ATP1 Publication

    Vmax=0.34 pmol/min/µg enzyme toward 1,2-dioleoyl-sn-glycerol1 Publication

    pH dependencei

    Optimum pH is 6.8.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: TAIR
    3. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. leaf development Source: TAIR
    3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    4. root development Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Plant defense, Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase 7 (EC:2.7.1.107)
    Short name:
    AtDGK7
    Short name:
    DAG kinase 7
    Alternative name(s):
    Diglyceride kinase 7
    Short name:
    DGK 7
    Gene namesi
    Name:DGK7
    Ordered Locus Names:At4g30340
    ORF Names:F17I23.320
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G30340.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Diacylglycerol kinase 7PRO_0000422115Add
    BLAST

    Proteomic databases

    PRIDEiF4JQ95.

    Expressioni

    Tissue specificityi

    Highly expressed in flowers, and at low levels in roots, stems and leaves.1 Publication

    Gene expression databases

    ArrayExpressiF4JQ95.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT4G30340.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliF4JQ95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini90 – 248159DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK00901.
    OMAiDECAREC.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR016961. Diacylglycerol_kinase_pln.
    [Graphical view]
    PfamiPF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    PIRSFiPIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
    SMARTiSM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F4JQ95-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEETPRSVGE ASTTNFVAAR PSAKTDDAVT MRGCGFANLA LVGIDKEELR    50
    GRLAMPEYLR IAMRDCIKRK DSTEISDHLL LPGGAAADMA PHAPMVVFIN 100
    PKSGGRHGPV LKERLQQLMT EEQVFDLTEV KPHEFVRYGL GCLDTLAAKG 150
    DECARECREK IRIMVAGGDG TVGWVLGCLG ELHKDGKSHI PPVGVIPLGT 200
    GNDLSRSFSW GGSFPFAWRS AMKRTLHRAT LGSIARLDSW KIVVSMPSGE 250
    VVDPPYSLKP TIEETALDQA LDADGDVPPK AKSYEGVFYN YFSIGMDAQV 300
    AYGFHHLRNE KPYLAQGPVT NKIIYSSYSC TQGWFCTPCV NNPALRGLRN 350
    IMKIHIKKAN CSEWEEIHVP KSVRSIVVLN LYNYGSGRHP WGNLRPKYLE 400
    KRGFVEAHCD DGLIEIFGLK QGWHASFVMA EIISAKHIAQ AAAIRFELRG 450
    GDWKNAFLQM DGEPWKQPMK SDYSTFVEIK KVPFQSLMIN GE 492
    Length:492
    Mass (Da):54,572
    Last modified:June 28, 2011 - v1
    Checksum:i7E5577CA2799D3A2
    GO

    Sequence cautioni

    The sequence AAK25884.1 differs from that shown. Reason: Intron retention.
    The sequence AAM44963.1 differs from that shown. Reason: Intron retention.
    The sequence AAU04880.1 differs from that shown. Reason: Intron retention.
    The sequence CAB81027.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti274 – 2741D → G in ABC71078. (PubMed:16081412)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY686593 mRNA. Translation: AAU04880.1. Sequence problems.
    DQ350135 mRNA. Translation: ABC71078.1.
    AL161576 Genomic DNA. Translation: CAB81027.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE85754.1.
    AF360174 mRNA. Translation: AAK25884.1. Sequence problems.
    AY113915 mRNA. Translation: AAM44963.1. Sequence problems.
    PIRiG85354.
    RefSeqiNP_567845.4. NM_119180.5.
    UniGeneiAt.4562.

    Genome annotation databases

    EnsemblPlantsiAT4G30340.1; AT4G30340.1; AT4G30340.
    GeneIDi829157.
    KEGGiath:AT4G30340.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY686593 mRNA. Translation: AAU04880.1 . Sequence problems.
    DQ350135 mRNA. Translation: ABC71078.1 .
    AL161576 Genomic DNA. Translation: CAB81027.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE85754.1 .
    AF360174 mRNA. Translation: AAK25884.1 . Sequence problems.
    AY113915 mRNA. Translation: AAM44963.1 . Sequence problems.
    PIRi G85354.
    RefSeqi NP_567845.4. NM_119180.5.
    UniGenei At.4562.

    3D structure databases

    ProteinModelPortali F4JQ95.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G30340.1-P.

    Proteomic databases

    PRIDEi F4JQ95.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G30340.1 ; AT4G30340.1 ; AT4G30340 .
    GeneIDi 829157.
    KEGGi ath:AT4G30340.

    Organism-specific databases

    TAIRi AT4G30340.

    Phylogenomic databases

    KOi K00901.
    OMAi DECAREC.

    Gene expression databases

    ArrayExpressi F4JQ95.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR016961. Diacylglycerol_kinase_pln.
    [Graphical view ]
    Pfami PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF030829. Diacylglycerol_kinase_pln. 1 hit.
    SMARTi SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
      Gomez-Merino F.C., Arana-Ceballos F.A., Trejo-Tellez L.I., Skirycz A., Brearley C.A., Doermann P., Mueller-Roeber B.
      J. Biol. Chem. 280:34888-34899(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    2. "Arabidopsis AtDGK7 regulates lateral root formation."
      Arana-Ceballos F.A., Gomez-Merino F., Krebs J., Skirycz A., Mueller-Roeber B.
      Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase."
      Arisz S.A., van Wijk R., Roels W., Zhu J.K., Haring M.A., Munnik T.
      Front. Plant Sci. 4:1-1(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDGK7_ARATH
    AccessioniPrimary (citable) accession number: F4JQ95
    Secondary accession number(s): B0KYU1, Q9C5L2, Q9M0C6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3