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F4JP36

- HAP2_ARATH

UniProt

F4JP36 - HAP2_ARATH

Protein

Protein HAPLESS 2

Gene

HAP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Involved in male fertility. Essential for pollen tube guidance, successful gamete attachment and fertilization.5 Publications

    GO - Biological processi

    1. pollen sperm cell differentiation Source: TAIR
    2. single fertilization Source: UniProtKB-KW

    Keywords - Biological processi

    Fertilization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein HAPLESS 2
    Alternative name(s):
    GENERATIVE CELL SPECIFIC 1
    Gene namesi
    Name:HAP2
    Synonyms:GCS1
    Ordered Locus Names:At4g11720
    ORF Names:T5C23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G11720.

    Subcellular locationi

    Endoplasmic reticulum membrane; Single-pass membrane protein. Cell membrane; Single-pass membrane protein
    Note: Predominantly localized in a perinuclear ring. Redistribution to the cell membrane is mediated by EC1 peptides after their secretion upon sperm arrival.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Defective in pollen tube guidance. Prevents gamete interaction and fertilization, resulting in male sterility.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 705681Protein HAPLESS 2PRO_0000416780Add
    BLAST

    Proteomic databases

    PRIDEiF4JP36.

    Expressioni

    Tissue specificityi

    Expressed only in mature pollen, in the two sperm cells.2 Publications

    Developmental stagei

    Expressed during pollen development and tube growth.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G11720.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliF4JP36.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 561537ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini583 – 705123CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei562 – 58221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi598 – 67174His-richAdd
    BLAST

    Domaini

    The N-terminal domain (62-541)and the C-terminal domain (583-705) are both required for functional gamete fusion. A positive charge, and not a conserved primary amino acid sequence, is required for a functional C-terminal domain. The N-terminal domain may be involved in interactions with another membrane-bound protein on female gametes, while positively charged C-terminus may function through electrostatic interactions with the sperm plasma membrane.2 Publications

    Sequence similaritiesi

    Belongs to the HAP2 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG77705.
    HOGENOMiHOG000084108.
    InParanoidiQ1AP31.
    OMAiCSAILKD.

    Family and domain databases

    InterProiIPR018928. Generative_cell_HAP2/GCS1.
    [Graphical view]
    PfamiPF10699. HAP2-GCS1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    F4JP36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNAILMACI LAGIFVGMFN EVDGIQILSK SKLEKCEKTS DSGNLNCSTK    50
    IVLNLAVPSG SSGGEASIVA EIVEVEDNSS SNMQTVRIPP VITVNKSAAY 100
    ALYDLTYIRD VPYKPQEYHV TTRKCEHDAG PDIVQICERL RDEKGNVLEQ 150
    TQPICCPCGP QRRMPSSCGD IFDKMIKGKA NTAHCLRFPG DWFHVFGIGQ 200
    RSLGFSVRVE LKTGTRVSEV IIGPENRTAT ANDNFLKVNL IGDFGGYTSI 250
    PSFEDFYLVI PREAAEAGQP GSLGANYSMW MLLERVRFTL DGLECNKIGV 300
    GYEAFNTQPN FCSSPYWSCL HNQLWNFRES DINRIDRHQL PLYGLEGRFE 350
    RINQHPNAGP HSFSIGVTET LNTNLMIELR ADDIEYVFQR SPGKIINIAI 400
    PTFEALTQFG VAAVIIKNTG EVEASYSLTF DCSKGVAFVE EQFFIIKPKA 450
    VTTRSFKLYP TKDQAAKYIC TAILKDSQFS EVDRAECQFS TTATVLDNGT 500
    QVTNPFQIPE TQPKGFFDSI RILWTKIING LVDFITGDTC RNKCSSFFDF 550
    SCHIQYVCLS WMVMFGLLLA LFPITCLLLW LLHQKGLFDP CYDWWEDHFD 600
    LDHHRRLLPS RADVVNRHHH HHKHRHHHNH HRRTHQRHKH HHGQDDDVLQ 650
    KMMLERDHSD SHYYHQLHRV HKDSKQKQRR RAKHGIVLPR DVHVERQRKQ 700
    RLRES 705
    Length:705
    Mass (Da):80,505
    Last modified:June 28, 2011 - v1
    Checksum:i0D4BEF577DDE6786
    GO

    Sequence cautioni

    The sequence CAB39943.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB78215.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti127 – 1271H → P in AAY51999. (PubMed:17079265)Curated
    Sequence conflicti127 – 1271H → P in AAY51998. (PubMed:17079265)Curated
    Sequence conflicti127 – 1271H → P in BAE71143. (PubMed:16378100)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ022375 mRNA. Translation: AAY51999.1.
    DQ022676 Genomic DNA. Translation: AAY51998.1.
    AB206811 mRNA. Translation: BAE71143.2.
    AL049500 Genomic DNA. Translation: CAB39943.1. Sequence problems.
    AL161532 Genomic DNA. Translation: CAB78215.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83041.1.
    PIRiT04219.
    RefSeqiNP_192909.2. NM_117241.2.
    UniGeneiAt.54282.

    Genome annotation databases

    EnsemblPlantsiAT4G11720.1; AT4G11720.1; AT4G11720.
    GeneIDi826777.
    KEGGiath:AT4G11720.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A wretched tale - Issue 146 of January 2013

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ022375 mRNA. Translation: AAY51999.1 .
    DQ022676 Genomic DNA. Translation: AAY51998.1 .
    AB206811 mRNA. Translation: BAE71143.2 .
    AL049500 Genomic DNA. Translation: CAB39943.1 . Sequence problems.
    AL161532 Genomic DNA. Translation: CAB78215.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83041.1 .
    PIRi T04219.
    RefSeqi NP_192909.2. NM_117241.2.
    UniGenei At.54282.

    3D structure databases

    ProteinModelPortali F4JP36.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G11720.1-P.

    Proteomic databases

    PRIDEi F4JP36.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G11720.1 ; AT4G11720.1 ; AT4G11720 .
    GeneIDi 826777.
    KEGGi ath:AT4G11720.

    Organism-specific databases

    TAIRi AT4G11720.

    Phylogenomic databases

    eggNOGi NOG77705.
    HOGENOMi HOG000084108.
    InParanoidi Q1AP31.
    OMAi CSAILKD.

    Family and domain databases

    InterProi IPR018928. Generative_cell_HAP2/GCS1.
    [Graphical view ]
    Pfami PF10699. HAP2-GCS1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization."
      von Besser K., Frank A.C., Johnson M.A., Preuss D.
      Development 133:4761-4769(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.
      Tissue: Pollen.
    2. "GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization."
      Mori T., Kuroiwa H., Higashiyama T., Kuroiwa T.
      Nat. Cell Biol. 8:64-71(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
      Strain: cv. Columbia.
      Tissue: Flower.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis hapless mutations define essential gametophytic functions."
      Johnson M.A., von Besser K., Zhou Q., Smith E., Aux G., Patton D., Levin J.Z., Preuss D.
      Genetics 168:971-982(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. "HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain."
      Wong J.L., Leydon A.R., Johnson M.A.
      PLoS Genet. 6:E1000882-E1000882(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN.
    7. "The functional domain of GCS1-based gamete fusion resides in the amino terminus in plant and parasite species."
      Mori T., Hirai M., Kuroiwa T., Miyagishima S.Y.
      PLoS ONE 5:E15957-E15957(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN.
    8. "Egg cell-secreted EC1 triggers sperm cell activation during double fertilization."
      Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U., Dresselhaus T.
      Science 338:1093-1097(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiHAP2_ARATH
    AccessioniPrimary (citable) accession number: F4JP36
    Secondary accession number(s): Q1AP31, Q2PGG6, Q9T0D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3