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F4JP36 (HAP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein HAPLESS 2
Alternative name(s):
GENERATIVE CELL SPECIFIC 1
Gene names
Name:HAP2
Synonyms:GCS1
Ordered Locus Names:At4g11720
ORF Names:T5C23
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in male fertility. Essential for pollen tube guidance, successful gamete attachment and fertilization. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Cell membrane; Single-pass membrane protein. Note: Predominantly localized in a perinuclear ring. Redistribution to the cell membrane is mediated by EC1 peptides after their secretion upon sperm arrival. Ref.1 Ref.2 Ref.8

Tissue specificity

Expressed only in mature pollen, in the two sperm cells. Ref.1 Ref.2

Developmental stage

Expressed during pollen development and tube growth. Ref.2

Domain

The N-terminal domain (62-541)and the C-terminal domain (583-705) are both required for functional gamete fusion. A positive charge, and not a conserved primary amino acid sequence, is required for a functional C-terminal domain. The N-terminal domain may be involved in interactions with another membrane-bound protein on female gametes, while positively charged C-terminus may function through electrostatic interactions with the sperm plasma membrane. Ref.6 Ref.7

Disruption phenotype

Defective in pollen tube guidance. Prevents gamete interaction and fertilization, resulting in male sterility. Ref.1 Ref.2

Sequence similarities

Belongs to the HAP2 family.

Sequence caution

The sequence CAB39943.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78215.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 705681Protein HAPLESS 2
PRO_0000416780

Regions

Topological domain25 – 561537Extracellular Potential
Transmembrane562 – 58221Helical; Potential
Topological domain583 – 705123Cytoplasmic Potential
Compositional bias598 – 67174His-rich

Experimental info

Sequence conflict1271H → P in AAY51999. Ref.1
Sequence conflict1271H → P in AAY51998. Ref.1
Sequence conflict1271H → P in BAE71143. Ref.2

Sequences

Sequence LengthMass (Da)Tools
F4JP36 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 0D4BEF577DDE6786

FASTA70580,505
        10         20         30         40         50         60 
MVNAILMACI LAGIFVGMFN EVDGIQILSK SKLEKCEKTS DSGNLNCSTK IVLNLAVPSG 

        70         80         90        100        110        120 
SSGGEASIVA EIVEVEDNSS SNMQTVRIPP VITVNKSAAY ALYDLTYIRD VPYKPQEYHV 

       130        140        150        160        170        180 
TTRKCEHDAG PDIVQICERL RDEKGNVLEQ TQPICCPCGP QRRMPSSCGD IFDKMIKGKA 

       190        200        210        220        230        240 
NTAHCLRFPG DWFHVFGIGQ RSLGFSVRVE LKTGTRVSEV IIGPENRTAT ANDNFLKVNL 

       250        260        270        280        290        300 
IGDFGGYTSI PSFEDFYLVI PREAAEAGQP GSLGANYSMW MLLERVRFTL DGLECNKIGV 

       310        320        330        340        350        360 
GYEAFNTQPN FCSSPYWSCL HNQLWNFRES DINRIDRHQL PLYGLEGRFE RINQHPNAGP 

       370        380        390        400        410        420 
HSFSIGVTET LNTNLMIELR ADDIEYVFQR SPGKIINIAI PTFEALTQFG VAAVIIKNTG 

       430        440        450        460        470        480 
EVEASYSLTF DCSKGVAFVE EQFFIIKPKA VTTRSFKLYP TKDQAAKYIC TAILKDSQFS 

       490        500        510        520        530        540 
EVDRAECQFS TTATVLDNGT QVTNPFQIPE TQPKGFFDSI RILWTKIING LVDFITGDTC 

       550        560        570        580        590        600 
RNKCSSFFDF SCHIQYVCLS WMVMFGLLLA LFPITCLLLW LLHQKGLFDP CYDWWEDHFD 

       610        620        630        640        650        660 
LDHHRRLLPS RADVVNRHHH HHKHRHHHNH HRRTHQRHKH HHGQDDDVLQ KMMLERDHSD 

       670        680        690        700 
SHYYHQLHRV HKDSKQKQRR RAKHGIVLPR DVHVERQRKQ RLRES 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization."
von Besser K., Frank A.C., Johnson M.A., Preuss D.
Development 133:4761-4769(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
Tissue: Pollen.
[2]"GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization."
Mori T., Kuroiwa H., Higashiyama T., Kuroiwa T.
Nat. Cell Biol. 8:64-71(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
Tissue: Flower.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis hapless mutations define essential gametophytic functions."
Johnson M.A., von Besser K., Zhou Q., Smith E., Aux G., Patton D., Levin J.Z., Preuss D.
Genetics 168:971-982(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain."
Wong J.L., Leydon A.R., Johnson M.A.
PLoS Genet. 6:E1000882-E1000882(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN.
[7]"The functional domain of GCS1-based gamete fusion resides in the amino terminus in plant and parasite species."
Mori T., Hirai M., Kuroiwa T., Miyagishima S.Y.
PLoS ONE 5:E15957-E15957(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN.
[8]"Egg cell-secreted EC1 triggers sperm cell activation during double fertilization."
Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U., Dresselhaus T.
Science 338:1093-1097(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Web resources

Protein Spotlight

A wretched tale - Issue 146 of January 2013

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ022375 mRNA. Translation: AAY51999.1.
DQ022676 Genomic DNA. Translation: AAY51998.1.
AB206811 mRNA. Translation: BAE71143.2.
AL049500 Genomic DNA. Translation: CAB39943.1. Sequence problems.
AL161532 Genomic DNA. Translation: CAB78215.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83041.1.
PIRT04219.
RefSeqNP_192909.2. NM_117241.2.
UniGeneAt.54282.

3D structure databases

ProteinModelPortalF4JP36.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G11720.1-P.

Proteomic databases

PRIDEF4JP36.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G11720.1; AT4G11720.1; AT4G11720.
GeneID826777.
KEGGath:AT4G11720.

Organism-specific databases

TAIRAT4G11720.

Phylogenomic databases

eggNOGNOG77705.
HOGENOMHOG000084108.
InParanoidQ1AP31.
OMACSAILKD.

Family and domain databases

InterProIPR018928. Generative_cell_HAP2/GCS1.
[Graphical view]
PfamPF10699. HAP2-GCS1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHAP2_ARATH
AccessionPrimary (citable) accession number: F4JP36
Secondary accession number(s): Q1AP31, Q2PGG6, Q9T0D9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names