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F4JP36

- HAP2_ARATH

UniProt

F4JP36 - HAP2_ARATH

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Protein

Protein HAPLESS 2

Gene

HAP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in male fertility. Essential for pollen tube guidance, successful gamete attachment and fertilization.5 Publications

GO - Biological processi

  1. pollen sperm cell differentiation Source: TAIR
  2. single fertilization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Protein HAPLESS 2
Alternative name(s):
GENERATIVE CELL SPECIFIC 1
Gene namesi
Name:HAP2
Synonyms:GCS1
Ordered Locus Names:At4g11720
ORF Names:T5C23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G11720.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass membrane protein. Cell membrane; Single-pass membrane protein
Note: Predominantly localized in a perinuclear ring. Redistribution to the cell membrane is mediated by EC1 peptides after their secretion upon sperm arrival.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 561537ExtracellularSequence AnalysisAdd
BLAST
Transmembranei562 – 58221HelicalSequence AnalysisAdd
BLAST
Topological domaini583 – 705123CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Defective in pollen tube guidance. Prevents gamete interaction and fertilization, resulting in male sterility.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 705681Protein HAPLESS 2PRO_0000416780Add
BLAST

Proteomic databases

PRIDEiF4JP36.

Expressioni

Tissue specificityi

Expressed only in mature pollen, in the two sperm cells.2 Publications

Developmental stagei

Expressed during pollen development and tube growth.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G11720.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4JP36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi598 – 67174His-richAdd
BLAST

Domaini

The N-terminal domain (62-541)and the C-terminal domain (583-705) are both required for functional gamete fusion. A positive charge, and not a conserved primary amino acid sequence, is required for a functional C-terminal domain. The N-terminal domain may be involved in interactions with another membrane-bound protein on female gametes, while positively charged C-terminus may function through electrostatic interactions with the sperm plasma membrane.2 Publications

Sequence similaritiesi

Belongs to the HAP2 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77705.
HOGENOMiHOG000084108.
InParanoidiF4JP36.
OMAiCSAILKD.

Family and domain databases

InterProiIPR018928. Generative_cell_HAP2/GCS1.
[Graphical view]
PfamiPF10699. HAP2-GCS1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F4JP36-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNAILMACI LAGIFVGMFN EVDGIQILSK SKLEKCEKTS DSGNLNCSTK
60 70 80 90 100
IVLNLAVPSG SSGGEASIVA EIVEVEDNSS SNMQTVRIPP VITVNKSAAY
110 120 130 140 150
ALYDLTYIRD VPYKPQEYHV TTRKCEHDAG PDIVQICERL RDEKGNVLEQ
160 170 180 190 200
TQPICCPCGP QRRMPSSCGD IFDKMIKGKA NTAHCLRFPG DWFHVFGIGQ
210 220 230 240 250
RSLGFSVRVE LKTGTRVSEV IIGPENRTAT ANDNFLKVNL IGDFGGYTSI
260 270 280 290 300
PSFEDFYLVI PREAAEAGQP GSLGANYSMW MLLERVRFTL DGLECNKIGV
310 320 330 340 350
GYEAFNTQPN FCSSPYWSCL HNQLWNFRES DINRIDRHQL PLYGLEGRFE
360 370 380 390 400
RINQHPNAGP HSFSIGVTET LNTNLMIELR ADDIEYVFQR SPGKIINIAI
410 420 430 440 450
PTFEALTQFG VAAVIIKNTG EVEASYSLTF DCSKGVAFVE EQFFIIKPKA
460 470 480 490 500
VTTRSFKLYP TKDQAAKYIC TAILKDSQFS EVDRAECQFS TTATVLDNGT
510 520 530 540 550
QVTNPFQIPE TQPKGFFDSI RILWTKIING LVDFITGDTC RNKCSSFFDF
560 570 580 590 600
SCHIQYVCLS WMVMFGLLLA LFPITCLLLW LLHQKGLFDP CYDWWEDHFD
610 620 630 640 650
LDHHRRLLPS RADVVNRHHH HHKHRHHHNH HRRTHQRHKH HHGQDDDVLQ
660 670 680 690 700
KMMLERDHSD SHYYHQLHRV HKDSKQKQRR RAKHGIVLPR DVHVERQRKQ

RLRES
Length:705
Mass (Da):80,505
Last modified:June 28, 2011 - v1
Checksum:i0D4BEF577DDE6786
GO

Sequence cautioni

The sequence CAB39943.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB78215.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271H → P in AAY51999. (PubMed:17079265)Curated
Sequence conflicti127 – 1271H → P in AAY51998. (PubMed:17079265)Curated
Sequence conflicti127 – 1271H → P in BAE71143. (PubMed:16378100)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ022375 mRNA. Translation: AAY51999.1.
DQ022676 Genomic DNA. Translation: AAY51998.1.
AB206811 mRNA. Translation: BAE71143.2.
AL049500 Genomic DNA. Translation: CAB39943.1. Sequence problems.
AL161532 Genomic DNA. Translation: CAB78215.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83041.1.
PIRiT04219.
RefSeqiNP_192909.2. NM_117241.2.
UniGeneiAt.54282.

Genome annotation databases

EnsemblPlantsiAT4G11720.1; AT4G11720.1; AT4G11720.
GeneIDi826777.
KEGGiath:AT4G11720.

Cross-referencesi

Web resourcesi

Protein Spotlight

A wretched tale - Issue 146 of January 2013

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ022375 mRNA. Translation: AAY51999.1 .
DQ022676 Genomic DNA. Translation: AAY51998.1 .
AB206811 mRNA. Translation: BAE71143.2 .
AL049500 Genomic DNA. Translation: CAB39943.1 . Sequence problems.
AL161532 Genomic DNA. Translation: CAB78215.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE83041.1 .
PIRi T04219.
RefSeqi NP_192909.2. NM_117241.2.
UniGenei At.54282.

3D structure databases

ProteinModelPortali F4JP36.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G11720.1-P.

Proteomic databases

PRIDEi F4JP36.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G11720.1 ; AT4G11720.1 ; AT4G11720 .
GeneIDi 826777.
KEGGi ath:AT4G11720.

Organism-specific databases

TAIRi AT4G11720.

Phylogenomic databases

eggNOGi NOG77705.
HOGENOMi HOG000084108.
InParanoidi F4JP36.
OMAi CSAILKD.

Family and domain databases

InterProi IPR018928. Generative_cell_HAP2/GCS1.
[Graphical view ]
Pfami PF10699. HAP2-GCS1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization."
    von Besser K., Frank A.C., Johnson M.A., Preuss D.
    Development 133:4761-4769(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
    Tissue: Pollen.
  2. "GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization."
    Mori T., Kuroiwa H., Higashiyama T., Kuroiwa T.
    Nat. Cell Biol. 8:64-71(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
    Tissue: Flower.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis hapless mutations define essential gametophytic functions."
    Johnson M.A., von Besser K., Zhou Q., Smith E., Aux G., Patton D., Levin J.Z., Preuss D.
    Genetics 168:971-982(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain."
    Wong J.L., Leydon A.R., Johnson M.A.
    PLoS Genet. 6:E1000882-E1000882(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.
  7. "The functional domain of GCS1-based gamete fusion resides in the amino terminus in plant and parasite species."
    Mori T., Hirai M., Kuroiwa T., Miyagishima S.Y.
    PLoS ONE 5:E15957-E15957(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.
  8. "Egg cell-secreted EC1 triggers sperm cell activation during double fertilization."
    Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U., Dresselhaus T.
    Science 338:1093-1097(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiHAP2_ARATH
AccessioniPrimary (citable) accession number: F4JP36
Secondary accession number(s): Q1AP31, Q2PGG6, Q9T0D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3